Integration of Multiomics Data Reveals Selection Characteristics of ITGB1 That Are Associated with Size Differentiation in Pigs
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(4), С. 1569 - 1569
Опубликована: Фев. 13, 2025
Min
pigs,
a
prominent
local
breed
from
Northeast
China,
have
diverged
into
two
distinct
breeds,
Ermin
(EM)
pigs
and
Hebao
(HB)
through
prolonged
natural
artificial
selection.
Although
these
breeds
exhibit
differences
in
body
size,
the
genetic
mechanisms
underlying
this
variation
remain
poorly
understood.
In
study,
we
performed
whole-genome
resequencing
transcriptome
analysis
on
EM
HB
to
elucidate
basis
of
size
genome-wide
selection
signal
identification
differentially
expressed
genes
(DEGs).
The
diversity
population
structure
across
14
pig
revealed
that,
compared
with
other
present
relatively
high
unique
structure.
Notably,
exhibited
significant
differentiation
pigs.
Integrated
data
candidate
associated
including
ENPP1,
ENPP3,
SPP1,
CLU,
ITGA11,
ITGB1,
IQGAP2,
BMP7,
F2RL2.
These
are
enriched
primarily
pathways
related
ECM-receptor
interactions;
pantothenate
CoA
biosynthesis;
starch
sucrose
metabolism;
nicotinate
nicotinamide
pyrimidine
nucleotide
cellular
responses
lipids;
biomineral
tissue
development;
biomineralization;
cell
signaling,
metabolic
responses,
lipid
deposition,
skeletal
development.
ITGB1
chromosome
10
showed
strong
positive
an
SNP
locus
exhibiting
G/A
allele
frequency
difference
between
(G
=
52.94%,
A
47.06%)
0%,
100%).
Our
findings
suggest
that
potentially
modulate
metabolism
efficiency
adipose
variations
expression
gene,
contributing
differences.
results
provide
new
insights
domestic
serve
as
valuable
reference
for
identifying
breeding
sizes.
Язык: Английский
The hippo pathway: a molecular bridge between environmental cues and pace of life
BMC Ecology and Evolution,
Год журнала:
2025,
Номер
25(1)
Опубликована: Апрель 24, 2025
Abstract
The
pace
of
life
(POL)
is
shaped
by
a
complex
interplay
between
genetic
and
environmental
factors,
influencing
growth,
maturation,
lifespan
across
species.
Hippo
signaling
pathway,
key
regulator
organ
size
cellular
homeostasis,
has
emerged
as
central
integrator
cues
that
modulate
POL
traits.
In
this
review,
we
explore
how
the
pathway
links
factors—such
temperature
fluctuations
dietary
energy
availability—to
molecular
mechanisms
governing
metabolic
balance,
hormonal
signaling,
reproductive
timing.
Specifically,
highlight
regulatory
interactions
sensors
(AMPK,
mTOR,
SIRT1
DLK1-Notch),
well
signals
(IGF-1,
kisspeptin,
leptin,
cortisol,
thyroid
sex
steroids),
which
together
orchestrate
life-history
traits,
including
growth
rates,
sexual
with
particular
emphasis
on
their
role
in
Furthermore,
consider
its
potential
coordinator
POL-related
processes,
such
telomere
dynamics
epigenetic
mechanisms,
within
broader
network.
By
integrating
insights
from
biology
eco-evolutionary
perspectives,
propose
future
directions
to
dissect
pathway’s
regulation
taxa.
Understanding
these
will
provide
new
perspectives
organisms
adaptively
adjust
strategies
response
variability.
Язык: Английский
Accurate models and nutritional strategies for specific oxidative stress factors: Does the dose matter in swine production?
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology,
Год журнала:
2024,
Номер
15(1)
Опубликована: Янв. 26, 2024
Oxidative
stress
has
been
associated
with
a
number
of
physiological
problems
in
swine,
including
reduced
production
efficiency.
Recently,
although
there
increased
research
into
regulatory
mechanisms
and
antioxidant
strategies
relation
to
oxidative
stress-induced
pig
production,
it
remains
so
far
largely
unsuccessful
develop
accurate
models
nutritional
for
specific
factors.
Here,
we
discuss
the
dose
intensity
causes
involving
physiological,
environmental
dietary
factors,
recent
provide
theoretical
guidance
future
swine.
Язык: Английский
Differentially Expressed Genes in Cardiomyocytes of the First Camelized Mouse Model, Nrapc.255ins78 Mouse
Genes,
Год журнала:
2025,
Номер
16(2), С. 142 - 142
Опубликована: Янв. 24, 2025
Background/Objectives:
The
first
camelized
mouse
model
(Nrapc.255ins78)
was
developed
to
investigate
the
mechanisms
underlying
camels’
adaptation
extreme
environments.
Previous
studies
demonstrated
that
these
mice
exhibit
a
cold-resistant
phenotype,
characterized
by
increased
expression
of
inflammatory
cytokine-related
genes
in
heart
under
cold
stress.
Nebulin-related
anchoring
protein
(NRAP)
plays
critical
role
organizing
myofibrils
during
cardiomyocyte
development.
This
study
builds
on
prior
research
analyzing
transcriptomes
Nrapc.255ins78
non-stress
conditions
explore
origins
cytokine
responses
exposure.
Methods:
RNA
sequencing
performed
hearts
12-week-old
male
and
female
wild-type
control
mice.
Results:
Differential
analysis
identified
25
genes,
including
12
associated
with
cell
cycle
division,
all
consistently
downregulated
Nrapc.255ins78.
Notably,
calcium
integrin-binding
gene
(Cib3)
significantly
upregulated
(FDR
<
0.05;
p
0.001).
Conclusions:
These
differentially
expressed
suggest
altered
dynamics
cardiomyocytes
for
maintaining
homeostasis,
supporting
hypothesis
cytokines
exposure
may
represent
an
adaptive
response.
findings
provide
valuable
insights
into
genetic
temperature
camels
highlight
potential
pathways
enhancing
stress
resistance
other
mammals.
Язык: Английский
Adaptive Thermogenesis and Lipid Metabolism Modulation in Inguinal and Perirenal Adipose Tissues of Hezuo Pigs in Response to Low-Temperature Exposure
Cells,
Год журнала:
2025,
Номер
14(6), С. 392 - 392
Опубликована: Март 7, 2025
In
mammals,
exposure
to
low
temperatures
induces
white
adipose
tissue
(WAT)
browning
and
alters
lipid
metabolism
promote
thermogenesis,
thereby
maintaining
body
temperature.
However,
this
response
varies
across
different
depots.
study,
Hezuo
pigs
were
exposed
either
room
temperature
(23
±
2
°C)
or
(−15
for
periods
of
12
h,
24
48
5
d,
10
15
d.
Inguinal
fat
(IF)
perirenal
(PF)
collected
analyzed
using
hematoxylin
eosin
(HE)
staining,
transmission
electron
microscopy,
RT-qPCR,
RNA-seq.
Following
cryoexposure,
our
results
demonstrated
a
significant
increase
in
adipocyte
number
corresponding
decrease
cross-sectional
area
both
IF
PF
groups
from
h
While
numbers
elevated
at
these
changes
not
statistically
significant.
Moreover,
droplets
mitochondria
more
abundant,
the
mRNA
expression
levels
thermogenic
genes
UCP3
PGC-1α
significantly
higher
compared
control
group
during
h-10
d
cold
period.
No
observed
other
groups.
RNA-seq
data
indicated
that
peaked
on
day
low-temperature
treatment.
tissue,
is
mainly
regulated
by
such
as
FABP4,
WNT10B,
PCK1,
PLIN1,
LEPR,
ADIPOQ.
These
are
involved
classical
pathway
provide
energy
adaptation.
contrast,
like
ATP5F1A,
ATP5PO,
SDHB,
NDUFS8,
SDHA,
COX5A
play
roles
within
neurodegenerative
disease
pathway,
has
positive
impact
process
related
degenerative
diseases.
Further
investigation
needed
clarify
functions
candidate
explore
genetic
mechanisms
underlying
cold-resistance
traits
local
pig
populations.
Язык: Английский
Characterization of pig skeletal muscle transcriptomes in response to low temperature
Veterinary Medicine and Science,
Год журнала:
2022,
Номер
9(1), С. 181 - 190
Опубликована: Дек. 8, 2022
Abstract
Objectives
The
response
of
mammals
to
cold
environment
is
a
complex
physiological
activity,
and
its
underlying
mechanism
must
be
analyzed
from
multiple
perspectives.
Skeletal
muscle
an
important
thermogenic
tissue
that
maintains
body
temperature
in
mammals.
We
dissected
the
molecular
pig
skeletal
by
performing
comparative
transcriptome
analysis
Enshi
black
pig.
Methods
Three
pigs
were
subjected
acute
stress
(3
days),
three
acclimation
(58
used
as
controls.
RNA‐seq
was
screen
differentially
expressed
genes
(DEGs)
muscle.
Results
Using
methods,
we
identified
1241
DEGs
within
group
1886
group.
Prolonged
exposure
induced
more
gene
expression
changes.
A
total
540
key
cold‐responsive
found,
their
trends
consistent
Gene
pattern
showed
there
significant
differences
between
low‐temperature
treatment
groups
control
group,
also
individuals
after
long‐term
treatment.
Analysis
revealed
134
pathways
significantly
enriched
adaptation
98
71
shared
two
groups.
mainly
related
lipid,
amino
acid,
carbohydrate
metabolism;
signal
transduction;
endocrine,
immune,
nervous
system;
cardiovascular
disease;
infectious
diseases
caused
bacteria
or
viruses;
neurodegenerative
disease.
Conclusions
In
conclusion,
this
study
provides
insights
into
porcine
under
environment.
data
may
assist
further
research
on
exposure.
Язык: Английский
Applications of Next-Generation Sequencing Technologies and Statistical Tools in Identifying Pathways and Biomarkers for Heat Tolerance in Livestock
Veterinary Sciences,
Год журнала:
2024,
Номер
11(12), С. 616 - 616
Опубликована: Дек. 2, 2024
The
climate
change-associated
abnormal
weather
patterns
negatively
influences
the
productivity
and
performance
of
farm
animals.
Heat
stress
is
major
detrimental
factor
hampering
production,
causing
substantial
economic
loss
to
livestock
industry.
Therefore,
it
important
identify
heat-tolerant
breeds
that
can
survive
produce
optimally
in
any
given
environment.
To
achieve
this
goal,
a
clearer
understanding
genetic
differences
underlying
molecular
mechanisms
associated
with
change
impacts
heat
tolerance
are
prerequisite.
Adopting
next-generation
biotechnological
statistical
tools
like
whole
transcriptome
analysis,
metagenome
sequencing,
bisulphite
genome-wide
association
studies
(GWAS),
selection
signatures
provides
an
opportunity
goal.
Through
these
techniques,
possible
permanent
markers
for
tolerance,
by
incorporating
those
marker-assisted
breeding
selection,
target
livestock.
This
review
gives
overview
recent
advancements
assessing
using
such
‘omics’
approaches
models.
salient
findings
from
research
highlighted
several
candidate
biomarkers
have
potential
be
incorporated
into
future
heat-tolerance
studies.
Such
could
revolutionise
production
changing
scenario
support
food
demands
growing
human
population.
Язык: Английский