Novel Highly Sensitive and Specific Multiplex Real-Time Pcr Assay for Detection of Leishmania Donovani and Human Immunodeficiency Virus-1 Co-Infection
Опубликована: Янв. 1, 2025
Язык: Английский
An updated review on green synthesized nanoparticles to control insect pests
Journal of Pest Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 9, 2025
Язык: Английский
Molecular evidence of Bartonella spp. in biting midges of the genus Culicoides Latreille (Diptera: Ceratopogonidae) from Brazil
Acta Tropica,
Год журнала:
2025,
Номер
unknown, С. 107553 - 107553
Опубликована: Фев. 1, 2025
Язык: Английский
Bloodthirsty Bites: A Study of Sandfly Feeding Patterns in the Aegean Region of Türkiye
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 22, 2025
Abstract
Background
This
study
determined
the
blood-feeding
patterns
of
wild-caught
sandflies
collected
from
provinces
(Aydin
and
Mugla)
in
Aegean
region
Türkiye.
Adult
were
using
three
CDC
light
traps
per
village
August
October
2024.
A
total
280
sandfly
specimens
(194
females
86
males)
during
study.
Of
194
female
specimens,
38
(23
Aydin
15
found
to
be
blood-fed,
137
non-blood-fed,
19
gravid.
In
total,
DNA
was
extracted
blood-fed
Invitrogen
PureLink
genomic
isolation
kit.
To
determine
these
insects,
mitochondrial
cytochrome
b
(
cytb)
gene
amplified
via
multiplex
PCR
specific
primers.
Results
The
results
showed
that
23
sand
fly
sampled
locality
fed
mostly
cows
(60.5%)
followed
by
dogs
(21%)
chickens
(18.4%).
All
samples
blood
whereas
for
Mugla
locality,
53%
46%
birds.
Host
preference
also
subjected
ELISA
reaction
identify
whose
host
could
not
see
if
there
multiple
preferences.
Our
bird
primers
used
methods,
meal
chickens,
probably
due
low
amounts
ingested
having
been
digested
or
denatured.
However,
studies
clearly
elucidated
samples.
Despite
limitations
disadvantages
inherent
both
which
are
most
frequently
employed
analysis,
all
successfully
analyzed
present
study,
obtained
methods
highly
consistent
with
each
other.
Conclusion
conclusion,
best
our
knowledge,
this
is
first
analyse
methodologies
a
comparative
manner
relation
feeding
flies.
detection
field-caught
flies
has
potential
facilitate
more
comprehensive
understanding
eco-epidemiology
vector-borne
diseases,
thereby
contributing
planning
strategic
control
methods.
Язык: Английский
Synthesis and Biological Evaluation of 1,2-Diborane Derivatives as Mosquito Larvicides
Опубликована: Янв. 1, 2025
Язык: Английский
Novel Highly Sensitive and Specific Multiplex Real-Time PCR Assay for Detection of Leishmania donovani and Human Immunodeficiency Virus-1 Co-Infection
Diagnostic Microbiology and Infectious Disease,
Год журнала:
2025,
Номер
112(4), С. 116860 - 116860
Опубликована: Апрель 17, 2025
Язык: Английский
A 3D printed device for vibration-assisted separation of different-stage mosquito larvae
Instrumentation Science & Technology,
Год журнала:
2024,
Номер
unknown, С. 1 - 16
Опубликована: Окт. 22, 2024
Mosquitoes,
as
major
vectors
of
dangerous
diseases,
demand
effective
control
measures
for
public
health.
Larvicides,
while
offering
a
potential
solution,
can
exhibit
varying
effects
on
different
mosquito
species
and
developmental
stages
during
efficacy
studies.
Standardizing
ensuring
optimal,
synchronized
development
rates
various
larval
are
crucial
larvicidal
experiments
maximizing
pupal
production.
Manual
separation
larvae
is
labor
intensive,
time
consuming
error
prone.
Therefore,
this
study
aimed
to
investigate
the
use
stereolithography
3D-printed
device
larvae.
This
designed
have
three
modules
collect
based
their
radial
size
differences.
Each
module
was
equipped
with
small
vibration
motors
enable
vibration-assisted
filtration
prevent
clogging.
The
accuracy
studied
by
repeated
collected
samples
were
analyzed
under
an
optical
microscope.
demonstrated
be
orders
magnitude
faster
more
accurate
compared
manual
process.
A
bioassay
also
assay
impact
survival
L3
L4
specimens.
Overall,
it
shown
that
presented
carries
important
rapid
Язык: Английский