Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Май 10, 2023

The similarity of the Listeria innocua genome with monocytogenes and their presence in same niche may facilitate gene transfer between them. A better understanding mechanisms responsible for bacterial virulence requires an in-depth knowledge genetic characteristics these bacteria. In this context, draft whole sequences were completed on five L. isolated from milk dairy products Egypt. assembled screened antimicrobial resistance genes, plasmid replicons multilocus sequence types (MLST); phylogenetic analysis sequenced isolates was also performed. sequencing results revealed only one gene, fosX, isolates. However, carried 13 genes involved adhesion, invasion, surface protein anchoring, peptidoglycan degradation, intracellular survival, heat stress; all lacked Pathogenicity Island 1 (LIPI-1) genes. MLST assigned into type (ST), ST-1085; however, single nucleotide polymorphism (SNP)-based 422-1,091 SNP differences our global lineages innocua. possessed ATP-dependent protease (clpL) which mediates resistance, a rep25 plasmids. Blast clpL-carrying contigs showed approximately 99% to corresponding parts plasmids strains 2015TE24968 N1-011A previously Italy United States, respectively. Although has been linked that serious outbreak, is first report containing Various among species other genera could raise possibility evolution virulent Such challenge processing preservation protocols pose health risks products. Ongoing genomic research necessary identify alarming changes develop preventive control measures.

Язык: Английский

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Listeria innocua isolated from diseased ruminants harbour minor virulence genes of L. monocytogenes

Veterinary Medicine and Science, Год журнала: 2022, Номер 8(2), С. 735 - 740

Опубликована: Янв. 18, 2022

Abstract Listeriosis is one of the most common nervous diseases in ruminants, and caused almost exclusively by Gram‐positive bacterium, Listeria monocytogenes . However, there are few reports listeriosis associated with L. innocua , which genetically closely related to but considered non‐pathogenic. In this work, we report two cases suppurative meningoencephalitis apparently previously healthy ruminants from different farms, strains were recovered. The whole genomes both isolates sequenced, allowing phylogenetic analyses be performed, indicated that very related. Virulence determinants searched, especially genes coding for main virulence factors have been described Surprisingly, do not possess such determinants. Instead, carried a set encode other genus detected using Factor Database (VFDB): iap (division invasion host cells), lpeA (entry into non‐professional phagocytes fbpA (multifunctional factor, including adherence cells) lspA (surface protein anchoring), lap (adhesion enterocytes trans epithelial translocation) pdgA (resistance lysozyme), oatA antimicrobial compounds also required growth inside macrophages) lplA1 (use host‐metabolites vivo growth), gtcA (catalyses teichoic acid bacterial wall), prsA2 (cell invasion, vacuole lysis intracellular clpC, clpE clpP (survival under several stress conditions). These among others detected, could involved ability produce damage animal human hosts. results highlight multifactorial profile pathogenesis need comprehensive scientific research address microbiological, environmental veterinary aspects listeriosis.

Язык: Английский

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Phage-Based Control of Listeria innocua in the Food Industry: A Strategy for Preventing Listeria monocytogenes Persistence in Biofilms

Viruses, Год журнала: 2025, Номер 17(4), С. 482 - 482

Опубликована: Март 27, 2025

Listeria innocua, though considered non-pathogenic, frequently coexists with monocytogenes in industrial environments, aiding its survival biofilms. These biofilms pose a significant challenge food processing facilities, as they protect bacteria from disinfectants and facilitate their spread. The aim of this review was to identify bacteriophages promising method for eliminating the industry. Lytic show great potential combating Commercially available products, such PhageGuard Listex™ (P100) (Micreos Food Safety, Wageningen, Netherlands), effectively reduce both L. innocua products on production surfaces. Additionally, phage-derived enzymes, endolysins, can degrade biofilms, without compromising quality. following article highlights that although present biocontrol method, further research is necessary assess long-term effectiveness, particularly regarding bacterial resistance. To maximize efficacy, combination strategies phage cocktails recommended enhance biofilm eradication minimize contamination risks.

Язык: Английский

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Identification and characterization of Listeria monocytogenes and Listeria innocua in bovine listeriosis cases in Uruguay

Tropical Animal Health and Production, Год журнала: 2025, Номер 57(3)

Опубликована: Апрель 1, 2025

Язык: Английский

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Chemical and Biological Studies of Achillea setacea Herba Essential Oil—First Report on Some Antimicrobial and Antipathogenic Features

Antibiotics, Год журнала: 2023, Номер 12(2), С. 371 - 371

Опубликована: Фев. 10, 2023

The essential oil of Achillea setacea was isolated by hydrodistillation and characterized GC-MS. antioxidant antimicrobial activity evaluated, as well its biocompatibility (LDH MTT methods). DPPH, FRAP, CUPRAC methods were applied for evaluation, while qualitative quantitative assays (inhibition zone diameter, minimum inhibitory concentration, fungicidal concentration), NO release (by nitrite concentration determination), microbial adhesion capacity to the inert substrate (the biofilm microtiter method) used investigate potential. A total 52 compounds identified GC-MS in A. oil, representing 97.43% area. major constituents borneol (32.97%), 1,8-cineole (14.94%), camphor (10.13%), artemisia ketone (4.70%), α-terpineol (3.23%), γ-eudesmol (3.23%). With MICs ranging from 0.78 30 μg/mL, proved inhibit induce release. To best our knowledge, present study reports first time EO against clinically biotechnologically important strains, such Shigella flexneri, Listeria ivanovii, L. innocua, Saccharomyces cerevisiae, Candida glabrata, Aspergillus niger, Rhizopus nigricans, Cladosporium cladosporioides, Alternaria alternata, demonstrating applications beyond clinical field.

Язык: Английский

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Preharvest Environmental and Management Drivers of Multidrug Resistance in Major Bacterial Zoonotic Pathogens in Pastured Poultry Flocks

Microorganisms, Год журнала: 2022, Номер 10(9), С. 1703 - 1703

Опубликована: Авг. 24, 2022

Due to nutritional benefits and perceived humane ways of treating the animals, demand for antibiotic-free pastured poultry chicken has continued be steadily rise. Despite non-usage antibiotics in broiler production, antibiotic resistance (AR) is reported zoonotic pathogens. However, factors that drive multidrug (MDR) are not well understood. In this study, we used machine learning deep approaches predict farm management practices physicochemical properties feces soil MDR Antibiotic use agroecosystems known contribute resistance. Evaluation development environments free such as all-natural, antibiotic-free, production systems described here critical understand background AR absence any selection pressure, i.e., basal levels We analyzed 1635 preharvest (feces soil) samples collected from forty-two flocks eleven farms Southeastern United States. CDC National Antimicrobial Resistance Monitoring System guidelines were determine antimicrobial/multidrug profiles Salmonella, Listeria, Campylobacter. A combination two traditional (RandomForest XGBoost) three (Multi-layer Perceptron, Generative Adversarial Network, Auto-Encoder) identified environmental variables pathogens represents This study enumerates makes recommendations potentially mitigate prevalence Salmonella Listeria poultry.

Язык: Английский

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Effects and Mechanisms of Resveratrol on the Adhesion of Lactobacillus acidophilus NCFM

Probiotics and Antimicrobial Proteins, Год журнала: 2022, Номер 15(6), С. 1529 - 1538

Опубликована: Ноя. 14, 2022

Язык: Английский

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Genetic diversity and known virulence genes in Listeria innocua strains isolated from cattle abortions and farm environment

Veterinary and Animal Science, Год журнала: 2022, Номер 19, С. 100276 - 100276

Опубликована: Дек. 1, 2022

Listeria innocua is considered as non-pathogenic bacteria living in an environment although several cases of immunocompromised humans and ruminant listeriosis infections have been reported. Previously, L. was identified a potential pathogen virulence association with monocytogenes PrfA dependent (LIPI-1) gene cluster demonstrated hemolytic innocua. usually versus pathogenic ivanovii because the main loss. There are limited studies reports available about innocua-caused illness cattle. A total 18 STs were cattle abortions while 17 farm majority present both environmental samples. Genome sequencing showed that one identical clones represented water, feed, soil, faeces sample groups, suggesting animals most likely through faecal shedding may remain source environment. Out all isolates PrfA-dependent genes not found aborted foetuses isolate groups; however, 20% complete LIPI-3 pathogenicity island encoding listeriolysin S identified. In this study, we genetically diverse widely distributed certain had significant for cattle, thus causing adverse health effects, including abortions.

Язык: Английский

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Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18

Deleted Journal, Год журнала: 2023, Номер 13(2), С. 611 - 620

Опубликована: Апрель 14, 2023

The Mediterranean diet has long been recognized as one of the most effective ways to prevent and improve cardiovascular disease. Extra virgin olive oil (EVOO) is typical sources fat in which have shown noteworthy nutritional value positive impact on human health. It worth noting that EVOO owes its superior bioactive composition. main component monounsaturated fatty acids (MUFAs) form oleic acid. Oleic acid accounts for up 70%-80% EVOO. Secondly, contains approximately more than 30 phenolic compounds, HT essential protection against CVDs. In this review, we focused potential mechanisms polyphenols combat diseases risk terms oxidative stress, inflammation, blood pressure, endothelial function cholesterol. This review might provide a reference studies protective effects

Язык: Английский

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Listeria innocua and serotypes of Listeria monocytogenes isolated from clinical cases in small ruminants in the northwest of Uruguay

Pesquisa Veterinária Brasileira, Год журнала: 2023, Номер 43

Опубликована: Янв. 1, 2023

ABSTRACT: Listeriosis is an infectious disease caused by bacteria of the genus Listeria, neurological form being more common in ruminants. There are many reports listeriosis small ruminants region that includes Brazil, Argentina and Uruguay. However, these diagnoses were mainly based on histological lesions central nervous system (CNS) without isolation characterization involved Listeria strains. The aim this study was to report sheep goats cases from 2016 2021 northwestern diagnosis made according observed at histopathology, plus CNS, identifying it specie serotype level. Nine animals (n=9) three outbreaks five sporadic studied. Sheep species with relation goats, adults category most affected. Cases occurred spring less frequently winter. All presented clinical signs CNS consistent suppurative meningoencephalitis micro-abscesses brainstem. In eight nine samples, strains isolated (seven L. monocytogenes one innocua). isolates carried inlA gene; serotyping showed four belonged 1/2b, two 4b, 1/2a. Considering a fact scarcely recovered ruminants, would be important emphasize need for better characterize affect animals. Not only improve knowledge about epidemiology but also objective developing specific vaccines animal use.

Язык: Английский

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