Comparison of Two Synthesis Methods for 3D PLA-Ibuprofen Nanofibrillar Scaffolds

Pharmaceutics, Год журнала: 2025, Номер 17(1), С. 106 - 106

Опубликована: Янв. 14, 2025

Objectives: This study aimed to synthesize polylactic acid (PLA) nanofibrillar scaffolds loaded with ibuprofen (IBU) using electrospinning (ES) and air-jet spinning (AJS). The were evaluated for their physicochemical properties, drug release profiles, biocompatibility assess potential local analgesic applications. Methods: Solutions of 10% (w/v) PLA combined IBU at concentrations 10%, 20%, 30% processed into membranes ES AJS. characterized scanning electron microscopy (SEM), differential calorimetry (DSC), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) spectroscopy. profile was assessed by ultraviolet-visible spectrophotometry (UV-Vis), cell adhesion viability fibroblast culture assays. Statistical analyses included qualitative analyses, t-tests, Likelihood ratio tests. Results: SEM revealed randomly arranged nanofibers forming reticulated meshes, more uniform dimensions observed in the AJS group. TGA DSC confirmed thermodynamic stability enthalpy changes consistent incorporation, which FT-IR UV-Vis validated. Drug sustained over 384 h, showing no significant differences between (p > 0.05). Cytotoxicity assays scaffold biocompatibility, cellular responses proportional concentration but within safe limits. Conclusions: PLA-IBU successfully synthesized Both methods yielded biocompatible systems stable properties controlled release. Further, vivo studies are necessary confirm clinical potential.

Язык: Английский

---

Modulating Cell Adhesion and Infiltration in Advanced Scaffold Designs based on PLLA Fibers with rGO and MXene (Ti3C2T )

Materials Today Bio, Год журнала: 2025, Номер unknown, С. 101785 - 101785

Опубликована: Апрель 1, 2025

The development of electrospun scaffolds that support cell adhesion and infiltration remains a critical challenge in tissue engineering. In this study, we investigate the influence two-dimensional (2D) fillers-reduced graphene oxide (rGO) MXene (Ti3C2T x )-incorporated into poly(L-lactic acid) (PLLA) fibers on their properties osteoblast responses. presence fillers modified fiber arrangement created varying inter-fiber spacing due to surface charge repulsion agglomeration. Importantly, potential measurements via Kelvin probe force microscopy (KPFM) PLLA show significant shift caused by incorporation Ti3C2T ∼400 mV compared ∼50 for rGO. vitro tests indicate rGO-modified up ∼100 μm, unlike fibers, which limit maximum ∼70 μm. However, promotes even deeper (∼120 μm) more uniform cell's changes scaffold architecture. High-resolution confocal imaging confirmed PLLA-Ti3C2T fosters larger, elongated site clusters cells, whereas rGO increases density relation without any filler. Our findings highlight distinct roles modulating geometry, mechanical behavior, cellular interactions. Tailoring composition distribution conductive offers promising strategy optimizing performance engineering applications.

Язык: Английский

---

Spatiotemporally-Patterned Biomaterials for Organoid Culture

Current Opinion in Biomedical Engineering, Год журнала: 2025, Номер unknown, С. 100594 - 100594

Опубликована: Май 1, 2025

Язык: Английский

---