Understanding GEMIN5 Interactions: From Structural and Functional Insights to Selective Translation
Wiley Interdisciplinary Reviews - RNA,
Год журнала:
2025,
Номер
16(2)
Опубликована: Март 1, 2025
ABSTRACT
GEMIN5
is
a
predominantly
cytoplasmic
protein,
initially
identified
as
member
of
the
survival
motor
neurons
(SMN)
complex.
In
addition,
this
abundant
protein
modulates
diverse
aspects
RNA‐dependent
processes,
executing
its
functions
through
formation
multi‐component
complexes.
The
modular
organization
structural
domains
present
in
enables
to
perform
various
interaction
with
distinct
partners.
responsible
for
recognition
small
nuclear
(sn)RNAs
N‐terminal
region,
and
therefore
snRNP
assembly.
Beyond
role
spliceosome
assembly,
regulates
translation
either
RNAs
or
proteins.
central
robust
dimerization
domain
acts
hub
protein–protein
interaction,
while
non‐canonical
RNA‐binding
site
located
towards
C‐terminus.
Interestingly,
partitioning
mRNAs
into
polysomes,
likely
due
capacity
ability
bind
native
ribosomes.
Understanding
functional
has
brought
an
increasing
interest
last
years
important
implications
human
disease.
Patients
carrying
biallelic
variants
suffer
from
neurodevelopmental
delay,
hypotonia,
cerebellar
ataxia.
This
review
discusses
recent
relevant
works
aimed
at
understanding
molecular
mechanisms
activity
gene
expression,
also
challenges
discover
new
functions.
Язык: Английский
Ribosome profiling reveals that post-transcriptional control of Nalf1 by heterogeneous nuclear ribonucleoprotein L is required for paclitaxel-induced neuropathic pain
Pain,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 2, 2025
Abstract
Sensory
neurons
are
integral
to
the
genesis
and
maintenance
of
neuropathic
pain.
The
molecular
mechanisms
that
mediate
long-lived
changes
in
their
excitability
unclear.
Here,
we
leverage
functional
genomics
approaches
survey
RNA
abundance
translation
dorsal
root
ganglion
from
a
mouse
model
paclitaxel-induced
We
focus
specifically
on
females
as
paclitaxel
is
first-line
therapy
for
breast
cancer.
sequencing
data
indicate
substantially
more
occur
at
level
(n
=
404)
than
transcription
decay
109).
discovered
core
subunit
sodium
leak
channel
(NALCN)
channel,
auxiliary
factor
1
(NALF1),
preferentially
translated
response
paclitaxel.
This
effect
mediated
by
RNA-binding
protein
heterogeneous
nuclear
ribonucleoprotein
L
(HNRNP
L).
Heterogeneous
binds
14
base
CA-rich
element
(CARE)
Nalf1
3′
untranslated
region
(3′UTR).
Genetic
elimination
either
HNRNP
L,
CARE
motif,
or
pore-forming
nonselective
NALCN
diminishes
pain
amplification
vivo.
Collectively,
these
results
illustrate
an
situated
3′UTR
required
female
mice.
Язык: Английский
The RNA-binding protein CELF4 is a negative regulator of sensory neuron excitability and mechanical and heat behavioral sensitivity
Neurobiology of Pain,
Год журнала:
2025,
Номер
unknown, С. 100184 - 100184
Опубликована: Май 1, 2025
Язык: Английский
The Functional Diversity of Chromatin‐Associated RNA Binding Proteins in Transcriptional and Post‐Transcriptional Regulation
Wiley Interdisciplinary Reviews - RNA,
Год журнала:
2025,
Номер
16(3)
Опубликована: Май 1, 2025
ABSTRACT
RNA‐binding
proteins
(RBPs)
are
a
diverse
class
of
that
interact
with
their
target
RNA
molecules
to
regulate
gene
expression
at
the
transcriptional
and
post‐transcriptional
levels.
RBPs
contribute
almost
all
aspects
processing
sequence‐specific,
structure‐specific,
nonspecific
binding
modes.
Advances
in
our
understanding
mechanisms
RBP‐mediated
regulatory
networks
consisting
DNAs,
RNAs,
protein
complexes
association
between
these
human
diseases
have
been
made
very
recently.
Here,
we
discuss
“unconventional”
functions
regulation
by
focusing
on
cutting‐edge
investigations
chromatin‐associated
(ChRBPs).
We
briefly
introduce
examples
how
ChRBPs
influence
genomic
features
molecular
structures
level
transcription.
In
addition,
focus
various
biogenesis,
transportation,
stability
control,
translation
ability
circular
(circRNAs).
Lastly,
raise
several
questions
about
clinical
significance
potential
therapeutic
utility
disease‐relevant
RBPs.
Язык: Английский
Epigenetic modifications associated to diabetic peripheral neuropathic pain (Review)
Molecular Medicine Reports,
Год журнала:
2024,
Номер
31(1)
Опубликована: Ноя. 13, 2024
The
present
review
aimed
to
provide
an
update
on
the
scientific
progress
of
role
epigenetic
modifications
diabetic
peripheral
neuropathic
pain
(DPNP).
DPNP
is
a
devastating
and
troublesome
complication
diabetes
mellitus
(DM),
which
affects
one
third
patients
with
DM
causes
severe
hyperalgesia
allodynia,
leading
challenges
in
treatment
these
patients.
pathophysiology
multifactorial
not
yet
fully
understood
options
for
this
disease
are
currently
unsatisfactory.
underlying
mechanisms
have
largely
been
explored
animal
models
mechanism‑derived
approach
might
offer
potential
therapeutic‑target
attenuating
certain
phenotypes
DPNP.
Altered
gene
expression
levels
within
or
central
nervous
systems
(CNS)
crucial
mechanism
DPNP,
however,
transcriptional
genes
elucidated.
Epigenetic
modifications,
such
as
DNA
methylation
histone
(methylation,
acetylation,
phosphorylation),
can
alter
via
chromatin
remodeling.
Moreover,
it
has
reported
that
altering
CNS,
contributes
changes
both
sensitivity
pharmacological
efficacy
Therefore,
summarized
findings
relevant
literature
alterations
therapeutic
targeting
future
disease.
Язык: Английский