Low-biomass pyruvate production with engineered Vibrio natriegens is accompanied by parapyruvate formation DOI Creative Commons
Maurice Hädrich,

Clarissa Scheuchenegger,

Sören-Tobias Vital

и другие.

Microbial Cell Factories, Год журнала: 2025, Номер 24(1)

Опубликована: Март 28, 2025

Abstract Background Pyruvate is a precursor for various compounds in the chemical, drug, and food industries therefore an attractive target molecule microbial production processes. The fast-growing bacterium Vibrio natriegens excels with its specific substrate uptake rate as unconventional chassis industrial biotechnology. Here, we aim to exploit traits of V. pyruvate fermentations low biomass concentrations. Results We inactivated dehydrogenase complex Δ vnp12 , which harbors deletions prophage regions . resulting strain aceE was unable grow minimal medium glucose unless supplemented acetate. In shaking flasks, showed growth 1.16 ± 0.03 h − 1 produced 4.0 0.3 g Pyr L within 5 h. optimized parameters aerobic fermentation process applied constant maintenance feed 0.24 Ac resulted maximal concentration only 6.6 0.4 CDW yielded highly active resting cells (q S ) 3.5 0.2 Glc −1 41.0 1.8 volumetric productivity 4.1 Carbon balancing disclosed gap 30%, identified partly parapyruvate. Deletion ligK encoding HMG/CHA aldolase did not impact formation but plasmid-based overexpression negatively affected led 3-fold higher parapyruvate culture broth. Notably, also supernatants pyruvate-producing Corynebacterium glutamicum strain. Cell-free bioreactor experiments mimicking biological formation, pointing chemical reaction contributing synthesis. Conclusions engineered metabolically producing high at concentration. However, found that accompanied by well C. Parapyruvate seems be result conversion might supported biochemically reaction.

Язык: Английский

Low-biomass pyruvate production with engineered Vibrio natriegens is accompanied by parapyruvate formation DOI Creative Commons
Maurice Hädrich,

Clarissa Scheuchenegger,

Sören-Tobias Vital

и другие.

Microbial Cell Factories, Год журнала: 2025, Номер 24(1)

Опубликована: Март 28, 2025

Abstract Background Pyruvate is a precursor for various compounds in the chemical, drug, and food industries therefore an attractive target molecule microbial production processes. The fast-growing bacterium Vibrio natriegens excels with its specific substrate uptake rate as unconventional chassis industrial biotechnology. Here, we aim to exploit traits of V. pyruvate fermentations low biomass concentrations. Results We inactivated dehydrogenase complex Δ vnp12 , which harbors deletions prophage regions . resulting strain aceE was unable grow minimal medium glucose unless supplemented acetate. In shaking flasks, showed growth 1.16 ± 0.03 h − 1 produced 4.0 0.3 g Pyr L within 5 h. optimized parameters aerobic fermentation process applied constant maintenance feed 0.24 Ac resulted maximal concentration only 6.6 0.4 CDW yielded highly active resting cells (q S ) 3.5 0.2 Glc −1 41.0 1.8 volumetric productivity 4.1 Carbon balancing disclosed gap 30%, identified partly parapyruvate. Deletion ligK encoding HMG/CHA aldolase did not impact formation but plasmid-based overexpression negatively affected led 3-fold higher parapyruvate culture broth. Notably, also supernatants pyruvate-producing Corynebacterium glutamicum strain. Cell-free bioreactor experiments mimicking biological formation, pointing chemical reaction contributing synthesis. Conclusions engineered metabolically producing high at concentration. However, found that accompanied by well C. Parapyruvate seems be result conversion might supported biochemically reaction.

Язык: Английский

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