Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms

Nature Communications, Год журнала: 2016, Номер 7(1)

Опубликована: Апрель 14, 2016

Abstract Many bacteria produce extracellular and surface-associated components such as membrane vesicles (MVs), DNA moonlighting cytosolic proteins for which the biogenesis export pathways are not fully understood. Here we show that explosive cell lysis of a sub-population cells accounts liberation content in Pseudomonas aeruginosa biofilms. Super-resolution microscopy reveals also produces shattered fragments rapidly form MVs. A prophage endolysin encoded within R- F-pyocin gene cluster is essential lysis. Endolysin-deficient mutants defective MV production biofilm development, consistent with crucial role MVs other matrix components. Our findings reveal lysis, mediated through activity cryptic endolysin, acts mechanism bacterial

Язык: Английский

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The Extracellular Matrix of Staphylococcus aureus Biofilms Comprises Cytoplasmic Proteins That Associate with the Cell Surface in Response to Decreasing pH

mBio, Год журнала: 2014, Номер 5(5)

Опубликована: Сен. 3, 2014

ABSTRACT Biofilm formation by Staphylococcus aureus involves the of an extracellular matrix, but composition this matrix has been uncertain. Here we report that is largely composed cytoplasmic proteins reversibly associate with cell surface in a manner depends on pH. We propose model for biofilm which are released from cells stationary phase. These response to decreasing pH during formation. Rather than utilizing dedicated protein, S. appears recycle moonlight as components matrix. IMPORTANCE leading cause multiantibiotic-resistant nosocomial infections and often found growing catheters chronic wounds. important pathogenicity strategy enhances resistance antimicrobials, thereby limiting treatment options ultimately contributing increased morbidity mortality. Cells held together consists whole or part nature not well understood. postulate recycles cytoplasm form This strategy, moonlighting proteins, could allow enhanced flexibility adaptability forming biofilms under infection conditions promote mixed-species

Язык: Английский

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Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics

PLoS Pathogens, Год журнала: 2016, Номер 12(4), С. e1005606 - e1005606

Опубликована: Апрель 29, 2016

Malaria parasite infection is initiated by the mosquito-transmitted sporozoite stage, a highly motile invasive cell that targets hepatocytes in liver for infection. A promising approach to developing malaria vaccine use of proteins located on surface as antigens elicit humoral immune responses prevent establishment Very little P. falciparum genome has been considered potential targets, and candidate vaccines have almost exclusively based single antigens, generating need novel target identification. The most advanced date, RTS,S, subunit consisting portion major protein circumsporozoite (CSP), conferred limited protection Phase III trials, falling short community-established efficacy goals. In striking contrast seen current sterilizing immunity can be achieved immunization with radiation-attenuated sporozoites, suggesting more potent may achievable multivalent vaccine. Here, we provide comprehensive analysis date or secreted Plasmodium salivary gland sporozoites. We used chemical labeling isolate surface-exposed sporozoites identified these mass spectrometry. validated several also evidence components inner membrane complex are fact accessible antibodies live Finally, our spectrometry data first direct CSP TRAP glycosylated finding could impact selection antigens.

Язык: Английский

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The Diverse Functional Roles of Elongation Factor Tu (EF-Tu) in Microbial Pathogenesis

Frontiers in Microbiology, Год журнала: 2019, Номер 10

Опубликована: Окт. 24, 2019

Elongation factor thermal unstable Tu (EF-Tu) is a G protein that catalyzes the binding of aminoacyl-tRNA to A-site ribosome inside living cells. Structural and biochemical studies have described complex interactions needed effect canonical function. However, EF-Tu has evolved capacity execute diverse functions on extracellular surface both eukaryote prokaryote can traffic to, retained on, cell surfaces where interact with membrane receptors matrix plant animal Our structural indicate short linear motifs (SLiMs) in exposed, non-conserved regions molecule may play key role moonlighting ascribed this ancient, highly abundant protein. Here we explore relating pathogenesis bacteria examine putative SLiMs surface-exposed molecule.

Язык: Английский

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Experimental models to study intestinal microbes–mucus interactions in health and disease

FEMS Microbiology Reviews, Год журнала: 2019, Номер 43(5), С. 457 - 489

Опубликована: Июнь 1, 2019

A close symbiotic relationship exists between the intestinal microbiota and its host. critical component of gut homeostasis is presence a mucus layer covering gastrointestinal tract. Mucus viscoelastic gel at interface luminal content host tissue that provides habitat to protects epithelium. The review starts by setting up biological context underpinning need for experimental models study bacteria-mucus interactions in digestive environment. We provide an overview structure function mucins, their with bacteria (including commensal, probiotics pathogenic microorganisms) role modulating health disease states. then describe characteristics potentials currently available mechanisms interaction microbes, including vitro, ex vivo models. discuss limitations challenges facing this field research.

Язык: Английский

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Proteinaceous determinants of surface colonization in bacteria: bacterial adhesion and biofilm formation from a protein secretion perspective

Frontiers in Microbiology, Год журнала: 2013, Номер 4

Опубликована: Янв. 1, 2013

Bacterial colonization of biotic or abiotic surfaces results from two quite distinct physiological processes, namely bacterial adhesion and biofilm formation. Broadly speaking, a is defined as the sessile development microbial cells. Biofilm formation arises following but not all single cells adhering reversibly irreversibly engage inexorably into mode growth. Among molecular determinants promoting colonization, surface proteins are most functionally diverse active components. To be present on cell surface, though, protein must secreted in first place. Considering close association with their cognate secretion systems, secretome (which refers both to systems substrates) key concept apprehend related functions. The here considered light differences cell-envelope architecture between diderm-LPS (archetypal Gram-negative), monoderm Gram-positive) diderm-mycolate acid-fast) bacteria. Besides, engaged process regarded supramolecular structure well non-classical secretion. This state-of-the-art complement (the effectors) involved bacteria paves way for future research directions field.

Язык: Английский

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MoonProt: a database for proteins that are known to moonlight

Nucleic Acids Research, Год журнала: 2014, Номер 43(D1), С. D277 - D282

Опубликована: Окт. 16, 2014

Moonlighting proteins comprise a class of multifunctional in which single polypeptide chain performs multiple biochemical functions that are not due to gene fusions, RNA splice variants or pleiotropic effects. The known moonlighting perform variety diverse many different cell types and species, information about their structures is scattered publications. We have constructed the manually curated, searchable, internet-based MoonProt Database (http://www.moonlightingproteins.org) with over 200 been experimentally verified be proteins. availability this organized provides more complete picture what currently database will also aid researchers other fields, including determining genes identified genome sequencing projects, interpreting data from proteomics projects annotating protein sequence structural databases. In addition, can helpful understanding how novel functional sites evolved on an ancient scaffold, help design functions.

Язык: Английский

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An introduction to protein moonlighting

Biochemical Society Transactions, Год журнала: 2014, Номер 42(6), С. 1679 - 1683

Опубликована: Ноя. 17, 2014

Moonlighting proteins comprise a class of multifunctional in which single polypeptide chain performs multiple physiologically relevant biochemical or biophysical functions. Almost 300 have been found to moonlight. The known examples moonlighting include diverse types proteins, including receptors, enzymes, transcription factors, adhesins and scaffolds, different combinations functions are observed. expressed throughout the evolutionary tree function many pathways. Some can perform both simultaneously, but for others, protein's changes response environment. already identified, potential benefits might provide organism, such as through coordinating cellular activities, suggest that more likely be found. Continuing studies structures will aid predicting identified genome sequencing projects, interpreting results from proteomics experiments, understanding how pathways interact systems biology, annotating protein sequence structure databases, evolution design with novel

Язык: Английский

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Chaperonin 60: a paradoxical, evolutionarily conserved protein family with multiple moonlighting functions

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Год журнала: 2013, Номер 88(4), С. 955 - 987

Опубликована: Март 29, 2013

ABSTRACT Chaperonin 60 is the prototypic molecular chaperone, an essential protein in eukaryotes and prokaryotes, whose sequence conservation provides excellent basis for phylogenetic analysis. Escherichia coli chaperonin ( GroEL ), prototype of this family proteins, has established oligomeric‐structure‐based folding mechanism a defined population partners. However, there growing number examples proteins crystal structures oligomeric composition are at variance with , suggesting that additional complexities protein‐folding function should be expected. In addition, many organisms have multiple some which lost their ability. It emerging highly conserved evolved bewildering variety biological functions – known as moonlighting both within cell extracellular milieu. Indeed, organisms, it these been left after loss activity. This highlights major paradox biology 60. article reviews relationship between non‐folding (moonlighting) activities discusses current knowledge on evolution focusing domains involved attempt to understand evolutionarily ancient family.

Язык: Английский

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Why study moonlighting proteins?

Frontiers in Genetics, Год журнала: 2015, Номер 6

Опубликована: Июнь 19, 2015

OPINION article Front. Genet., 19 June 2015Sec. Computational Genomics https://doi.org/10.3389/fgene.2015.00211

Язык: Английский

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