Secretome of obligate intracellularRickettsia

FEMS Microbiology Reviews, Год журнала: 2014, Номер unknown, С. n/a - n/a

Опубликована: Авг. 28, 2014

The genus Rickettsia (Alphaproteobacteria, Rickettsiales, Rickettsiaceae) is comprised of obligate intracellular parasites, with virulent species interest both as causes emerging infectious diseases and for their potential deployment bioterrorism agents. Currently, there are no effective commercially available vaccines, treatment limited primarily to tetracycline antibiotics, although others (e.g. josamycin, ciprofloxacin, chloramphenicol, azithromycin) also effective. Much the recent research geared toward understanding mechanisms underlying rickettsial pathogenicity has centered on characterization secreted proteins that directly engage eukaryotic cells. Herein, we review all aspects secretome, including six secretion systems, 19 characterized secretory proteins, moonlighting identified surfaces multiple species. Employing bioinformatics phylogenomics, present novel structural functional insight each system. Unexpectedly, our investigation revealed majority have not been assigned cognate pathways. Furthermore, most pathways, requisite signal sequences mediating translocation poorly understood. As a blueprint known routes protein into host cells, this resource will assist aimed at uniting apposite work help in identification involved 'life inside'.

Язык: Английский

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Coordinating Bacterial Cell Division with Nutrient Availability: a Role for Glycolysis

mBio, Год журнала: 2014, Номер 5(3)

Опубликована: Май 14, 2014

ABSTRACT Cell division in bacteria is driven by a cytoskeletal ring structure, the Z ring, composed of polymers tubulin-like protein FtsZ. Z-ring formation must be tightly regulated to ensure faithful cell division, and several mechanisms that influence positioning timing assembly have been described. Another important but as yet poorly understood aspect regulation need coordinate with growth nutrient availability. In this study, we demonstrated for first time intimately linked central carbon metabolism model Gram-positive bacterium Bacillus subtilis . We showed deletion gene encoding pyruvate kinase ( pyk ), which produces final reaction glycolysis, rescues defect temperature-sensitive ftsZ mutant has significant effects on wild-type B. cells. Addition exogenous restores normal absence enzyme, implicating key metabolite coordination bacterial division. Our results support levels are coupled via an enzyme actually metabolizes pyruvate, E1α subunit dehydrogenase. shown localizes over nucleoid pyruvate-dependent manner may stimulate more efficient at center under nutrient-rich conditions, when cells divide frequently. IMPORTANCE How cycle processes availability fundamental unresolved question microbiology. Recent breakthroughs revealed nutritional information can transmitted directly from metabolic pathways machinery serve mechanism fine-tuning response changes environmental conditions. Here identified novel link between glycolysis product plays role maintaining Nutrient-dependent affect function FtsZ, most likely modifying activity namely, dehydrogenase E1α. Ultimately system help conditions survival newborn

Язык: Английский

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Proteomic profile of the skin mucus of farmed gilthead seabream (Sparus aurata)

Journal of Proteomics, Год журнала: 2015, Номер 120, С. 21 - 34

Опубликована: Март 6, 2015

Язык: Английский

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Moonlighting enzymes: when cellular context defines specificity

Cellular and Molecular Life Sciences, Год журнала: 2023, Номер 80(5)

Опубликована: Апрель 24, 2023

Язык: Английский

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Towards a processual microbial ontology

Biology & Philosophy, Год журнала: 2012, Номер 28(2), С. 379 - 404

Опубликована: Ноя. 5, 2012

Standard microbial evolutionary ontology is organized according to a nested hierarchy of entities at various levels biological organization. It typically detects and defines these in relation the most stable aspects processes, by identifying lineages evolving process vertical inheritance from an ancestral entity. However, recent advances microbiology indicate that such has important limitations. The dynamics detected within microbiological systems reveal focus on (or features entities) over time inevitably underestimates extent nature diversity. These are not outcome descent alone. Other often involving causal interactions between distinct organisation, or operating different scales, responsible only for destabilisation pre-existing entities, but also emergence stabilisation novel world. In this article we consider as more less stabilised functional wholes, sketch network-based can represent diverse set processes including, example, well phylogenetic relations, stabilise destabilise interacting spatial ecological connections, genetic exchanges. We use pluralistic framework evaluating (i) existing ontological assumptions evolution (e.g. whether currently recognized adequate understanding causes change world), (ii) hidden kinds, essentially invisible limited perspective. propose recognize additional classes provide new insights into structure world, namely "processually equivalent" versatile" "stabilized" entities.

Язык: Английский

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Physical Features of Intracellular Proteins that Moonlight on the Cell Surface

PLoS ONE, Год журнала: 2015, Номер 10(6), С. e0130575 - e0130575

Опубликована: Июнь 25, 2015

Moonlighting proteins comprise a subset of multifunctional that perform two or more biochemical functions are not due to gene fusions, multiple splice variants, proteolytic fragments, promiscuous enzyme activities. The project described herein focuses on sub-set moonlighting have canonical function inside the cell and second surface in at least one species. goal this is consider biophysical features these determine whether they shared characteristics defining might suggest why particular were adopted for surface, if resemble typical intracellular proteins. latter many other normally found also be fashion. We identified 30 types different surface. Some moonlight species, sometimes with extracellular so there total 98 study set. Although variety (enzymes, chaperones, etc.) observed re-used most part, physical Many been bacterial pathogens organisms proteomics experiments. It quite possible those increasing number known may than previously thought, common feature

Язык: Английский

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Role of Streptococcus pneumoniae Proteins in Evasion of Complement-Mediated Immunity

Frontiers in Microbiology, Год журнала: 2017, Номер 8

Опубликована: Фев. 20, 2017

The complement system plays a central role in immune defense against Streptococcus pneumoniae. In order to evade attack, pneumococci have evolved number of mechanisms that limit mediated opsonization and subsequent phagocytosis. This review focuses on the strategies employed by circumvent immunity, both vitro vivo. At last, since many proteins involved interactions with components are vaccine candidates different stages validation, we explore use these antigens alone or combination, as potential approaches aim at elimination drastic reduction ability this bacterium complement.

Язык: Английский

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Intracellular proteins moonlighting as bacterial adhesion factors

AIMS Microbiology, Год журнала: 2018, Номер 4(2), С. 362 - 376

Опубликована: Янв. 1, 2018

Pathogenic and commensal, or probiotic, bacteria employ adhesins on the cell surface to attach interact with host. Dozens of that play key roles in binding host cells extracellular matrix were originally identified as intracellular chaperones enzymes glycolysis other central metabolic pathways. Proteins have two very different functions, often subcellular locations, are referred moonlighting proteins. The intracellular/surface proteins do not contain signal sequences for secretion known sequence motifs surface, so most cases is how these secreted they become attached surface. A system which a large portion pool each protein remains inside while some partitioned has been identified. This may involve novel version it system. Understanding processes by intracellular/cell targeted could provide targets development small molecules block and/or association serve lead compounds antibacterial therapeutics.

Язык: Английский

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Bacterial Excretion of Cytoplasmic Proteins (ECP): Occurrence, Mechanism, and Function

Trends in Microbiology, Год журнала: 2018, Номер 27(2), С. 176 - 187

Опубликована: Ноя. 12, 2018

Язык: Английский

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Protein species and moonlighting proteins: Very small changes in a protein's covalent structure can change its biochemical function

Journal of Proteomics, Год журнала: 2015, Номер 134, С. 19 - 24

Опубликована: Окт. 15, 2015

Язык: Английский

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