Characterization of fluorescence lifetime of organic fluorophores for molecular imaging in the SWIR window DOI Creative Commons
Luis Chavez, Shan Gao, Xavier Intes

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Дек. 19, 2022

Abstract Significance Fluorescence lifetime imaging in the short-wave infrared (SWIR) is expected to enable high resolution multiplexed molecular highly scattering tissue. Aim To characterize brightness and fluorescence of commercially available organic SWIR fluorophores benchmark them against tail emission conventional NIR-excited probes. Approach Characterization was performed through our established Time-domain Mesoscopic Molecular Tomography (TD-MFMT) system integrated around a TCSPC-SPAD array. Brightness measured for NIR probes above 1000 nm. Simultaneous probe then assess their potential studies. Results outperformed while mean lifetimes were extremely short. The phantom study demonstrated feasibility multiplexing window with both Conclusions Long beyond readily detectable window, where showed shorter compared We demonstrate which paves way vivo studies intact tissues at improved resolution.

Язык: Английский

Fluorescence lifetime imaging in drug delivery research DOI Creative Commons
Yiqing Lu,

Parinaz Jabbari,

Anton Mukhamedshin

и другие.

Advanced Drug Delivery Reviews, Год журнала: 2025, Номер 218, С. 115521 - 115521

Опубликована: Янв. 22, 2025

Язык: Английский

Процитировано

2
Multiplexed near infrared fluorescence lifetime imaging in turbid media DOI Creative Commons

Meital Harel,

Uri Arbiv,

Rinat Ankri

и другие.

Journal of Biomedical Optics, Год журнала: 2024, Номер 29(02)

Опубликована: Фев. 29, 2024

SignificanceFluorescence lifetime imaging (FLI) plays a pivotal role in enhancing our understanding of biological systems, providing valuable tool for non-invasive exploration biomolecular and cellular dynamics, both vitro vivo. Its ability to selectively target multiplex various entities, alongside heightened sensitivity specificity, offers rapid cost-effective insights.AimOur aim is investigate the multiplexing capabilities near-infrared (NIR) FLI within scattering medium that mimics tissues. We strive develop comprehensive FLI's potential diverse targets complex, tissue-like environment.ApproachWe introduce an innovative Monte Carlo (MC) simulation approach accurately describes behavior fluorescent photons turbid media. Applying phasor analyses, we enable distinct single image. Leveraging state-of-the-art single-photon avalanche diode (SPAD) time-gated camera, SPAD512S, conduct experimental wide-field NIR regime.ResultsOur study demonstrates successful dual image, reaching depth 1 cm phantoms. Through novel MC showcase effectiveness methodology overcoming challenges posed by media.ConclusionsThis research underscores applications complex environments. By combining advanced techniques with cutting-edge tools, significant results advance field research.

Язык: Английский

Процитировано

4
Recent advances in ratiometric fluorescence imaging of enzyme activity in vivo DOI
Emily D. Cosco, Matthew Bogyo

Current Opinion in Chemical Biology, Год журнала: 2024, Номер 80, С. 102441 - 102441

Опубликована: Март 7, 2024

Язык: Английский

Процитировано

4
Design and Characterization of an Optical Phantom for Mesoscopic Multimodal Fluorescence Lifetime Imaging and Optical Coherence Elastography DOI Creative Commons
Luis Chavez, Shan Gao, Vikas Pandey

и другие.

Biomedical Optics Express, Год журнала: 2025, Номер 16(3), С. 1006 - 1006

Опубликована: Фев. 5, 2025

We developed a novel methodology for manufacturing multimodal, tissue-mimicking phantoms that exhibit both molecular and biomechanical contrast. This leverages the immiscibility of silicone hydrogels to create solid mesoscale with localized regions precisely controlled fluorescence, including fluorescence lifetime properties, adjustable stiffness, without requiring physical barriers. Mechanical, fluorescent, optical characterization confirmed tunability across range values relevant biomedical applications. A macroscale 3D phantom was fabricated, its properties were validated through imaging (FLI) coherence elastography (OCE). Validation demonstrated successful tuning mechanical contrasts within structure, highlighting feasibility multimodal FLI-OCE. new process is expected support development validation approaches study tumor microenvironment (TME), as well their impact on therapeutic efficacy, enhance targeted therapies.

Язык: Английский

Процитировано

0
Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor DOI Creative Commons
Alena Rudkouskaya, Nattawut Sinsuebphon, Marien Ochoa

и другие.

Theranostics, Год журнала: 2020, Номер 10(22), С. 10309 - 10325

Опубликована: Янв. 1, 2020

Following an ever-increased focus on personalized medicine, there is a continuing need to develop preclinical molecular imaging modalities guide the development and optimization of targeted therapies. Near-Infrared (NIR) Macroscopic Fluorescence Lifetime Förster Resonance Energy Transfer (MFLI-FRET) offers unique method robustly quantify receptor-ligand engagement in live intact animals, which critical assess delivery efficacy therapeutics. However, date, non-invasive approaches that can simultaneously measure cellular drug metabolic response are lacking. A major challenge for implementation concurrent optical MFLI-FRET

Язык: Английский

Процитировано

23
In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET DOI Creative Commons
Jason T. Smith, Nattawut Sinsuebphon, Alena Rudkouskaya

и другие.

Biophysical Reports, Год журнала: 2023, Номер 3(2), С. 100110 - 100110

Опубликована: Май 9, 2023

Förster resonance energy transfer (FRET) microscopy is used in numerous biophysical and biomedical applications to monitor inter- intramolecular interactions conformational changes the 2-10 nm range. FRET currently being extended vivo optical imaging, its main application quantifying drug-target engagement or drug release animal models of cancer using organic dye nanoparticle-labeled probes. Herein, we compared quantification intensity-based (sensitized emission analysis with three-cube approach an IVIS imager) macroscopic fluorescence lifetime (MFLI) a custom system time-gated-intensified charge-coupled device, for small imaging. The analytical expressions experimental protocols required quantify product

Язык: Английский

Процитировано

8
Applications of Machine Learning in time-domain Fluorescence Lifetime Imaging: a Review DOI Creative Commons
Dorian Gouzou, Ali Taimori, Tarek Haloubi

и другие.

Methods and Applications in Fluorescence, Год журнала: 2023, Номер 12(2), С. 022001 - 022001

Опубликована: Дек. 6, 2023

Many medical imaging modalities have benefited from recent advances in Machine Learning (ML), specifically deep learning, such as neural networks. Computers can be trained to investigate and enhance methods without using valuable human resources. In years, Fluorescence Lifetime Imaging (FLIm) has received increasing attention the ML community. FLIm goes beyond conventional spectral imaging, providing additional lifetime information, could lead optical histopathology supporting real-time diagnostics. However, most current studies do not use full potential of machine/deep learning models. As a developing image modality, data are easily obtainable, which, coupled with an absence standardisation, is pushing back research develop models which advance automated diagnosis help promote FLIm. this paper, we describe developments that improve quality, time-domain systems, summarise sensing, signal-to-noise analysis registration low-level tracking. We review two main applications for FLIm: estimation through classification segmentation. suggest course action quality applied Our final goal attract more practitioners explore imaging.

Язык: Английский

Процитировано

6
Fluorescence Lifetime Imaging for Quantification of Targeted Drug Delivery in Varying Tumor Microenvironments DOI Creative Commons
Amit Verma, Vikas Pandey,

Catherine Sherry

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 15, 2024

Trastuzumab (TZM) is a monoclonal antibody that targets the human epidermal growth factor receptor (HER2) and clinically used for treatment of HER2-positive breast tumors. However, tumor microenvironment can limit access TZM to HER2 across whole thereby compromise TZM's therapeutic efficacy. An imaging methodology non-invasively quantify binding TZM-HER2, which required action, distribution within tumors with varying microenvironments much needed. We performed near-infrared (NIR) fluorescence lifetime (FLI) Forster Resonance Energy Transfer (FRET) measure TZM-HER2 binding, using in vitro microscopy vivo widefield macroscopy, overexpressing ovarian cancer cells xenografts, respectively. Immunohistochemistry was validate results. NIR FLI FRET data show variations intracellular bound AU565 tumor-passaged XTM cell lines comparison SKOV-3 cells. Macroscopy (MFLI) display reduced compared tumors, as validated by ex immunohistochemistry. Moreover, AU565/XTM xenografts different amounts distributions TME components, such collagen vascularity. Therefore, these results suggest are refractory delivery due their disrupted vasculature increased content. Our study demonstrates powerful analytical tool monitor drug both cultures live systems. Especially, MFLI unique modality directly target engagement potential elucidate role efficacy intact xenografts.

Язык: Английский

Процитировано

2
3D k-space reflectance fluorescence tomography via deep learning DOI
Navid Ibtehaj Nizam, Marien Ochoa, Jason T. Smith

и другие.

Optics Letters, Год журнала: 2022, Номер 47(6), С. 1533 - 1533

Опубликована: Фев. 17, 2022

We report on the potential to perform image reconstruction in 3D k -space reflectance fluorescence tomography (FT) using deep learning (DL). Herein, we adopt a modified AUTOMAP architecture and develop training methodology that leverages an open-source Monte-Carlo-based simulator generate large dataset. Using enhanced EMNIST (EEMNIST) dataset as embedded contrast function allows us train network efficiently. The optical strategy utilizes illumination configuration probe tissue mesoscopic regime with high sensitivity resolution. proposed DL model validation is performed both silico data phantom experiment. Overall, our results indicate approach can correctly reconstruct single multiple fluorescent embedding(s) volume. Furthermore, presented technique shown outperform traditional approaches [least-squares (LSQ) total-variation minimization (TVAL)], especially at higher depths. We, therefore, expect computational have future implications preclinical studies.

Язык: Английский

Процитировано

10
Macroscopic temporally and spectrally resolved fluorescence imaging enhanced by laser-wavelength multiplexing DOI
Vladislav I. Shcheslavskiy, Diana V. Yuzhakova, Daria A. Sachkova

и другие.

Optics Letters, Год журнала: 2023, Номер 48(20), С. 5309 - 5309

Опубликована: Сен. 18, 2023

We present a laser scanning system for macroscopic samples that records fully resolved decay curves in individual pixels, resolves the images 16 wavelength channels, and simultaneously at several wavelengths. By using confocal detection, delivers are virtually free of lateral scattering out-of-focus haze. Image formats can be up to 256 × pixels 1024 time channels. demonstrate performance both on model experiments with fluorescent micro-beads tumor living mice.

Язык: Английский

Процитировано

5