Challenging the Astral mass analyzer to quantify up to 5,300 proteins per single cell at unseen accuracy to uncover cellular heterogeneity

Nature Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Язык: Английский

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An Overview of the Role of MicroRNAs on Carcinogenesis: A Focus on Cell Cycle, Angiogenesis and Metastasis

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(8), С. 7268 - 7268

Опубликована: Апрель 14, 2023

In recent years, the importance of epigenetic markers in carcinogenesis different malignant neoplasms has been demonstrated, also demonstrating their utility for understanding metastatic spread and tumor progression cancer patients. Among biomarkers, microRNAs represent a set non-coding RNAs that regulate gene expression, having involved wide variety neoplasia acting oncogenic pathways. Both overexpression downregulation complex interaction with various genes whose ultimate consequence is increased cell proliferation, invasion driver markers. It should be noted current clinical practice, even though combination shown to useful by authors at diagnostic prognostic levels, there are no kits can used initial approach or assess recurrences oncological diseases. Previous works have cited as critical role several carcinogenic mechanisms, ranging from cycle alterations angiogenesis mechanisms distant dissemination. Indeed, specific seem tightly modulation components related these processes. For instance, cyclins cyclin-dependent kinases, transcription factors, signaling molecules angiogenic/antiangiogenic products, among others, recognized targets types cancer. Therefore, purpose this article describe main implications alterations, metastasis angiogenesis, trying summarize involvement carcinogenesis.

Язык: Английский

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The pathophysiology of the cell cycle in cancer and treatment strategies using various cell cycle checkpoint inhibitors

Pathology - Research and Practice, Год журнала: 2023, Номер 251, С. 154854 - 154854

Опубликована: Окт. 4, 2023

Язык: Английский

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R-loop and diseases: the cell cycle matters

Molecular Cancer, Год журнала: 2024, Номер 23(1)

Опубликована: Апрель 27, 2024

Abstract The cell cycle is a crucial biological process that involved in growth, development, and reproduction. It can be divided into G1, S, G2, M phases, each period closely regulated to ensure the production of two similar daughter cells with same genetic material. However, many obstacles influence cycle, including R-loop formed throughout this process. triple-stranded structure, composed an RNA: DNA hybrid single strand, which ubiquitous organisms from bacteria mammals. existence has important significance for regulation various physiological processes. aberrant accumulation due its limited resolving ability will detrimental cells. For example, damage genomic instability, caused by R-loop, activate checkpoints turn induce arrest death. At present, growing number factors have been proven prevent or eliminate thereby avoiding mutations. Therefore, we need gain detailed insight resolution at different stages cycle. In review, review current knowledge play role as well how mutations these lead onset progression diseases.

Язык: Английский

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Exosomal delivery of rapamycin modulates blood-brain barrier penetration and VEGF axis in glioblastoma

Journal of Controlled Release, Год журнала: 2025, Номер unknown, С. 113605 - 113605

Опубликована: Март 1, 2025

Язык: Английский

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Challenging the Astral^™mass analyzer - up to 5300 proteins per single-cell at unseen quantitative accuracy to study cellular heterogeneity

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 1, 2024

ABSTRACT A detailed proteome map is crucial for understanding molecular pathways and protein functions. Despite significant advancements in sample preparation, instrumentation, data analysis, single-cell proteomics currently limited by proteomic depth quantitative performance. We combine a zero dead-end volume chromatographic column running at high throughput with the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer DIA mode. demonstrate unprecedented of coverage as well accuracy precision quantification ultra-low input amounts. Using tailored library, we identify up to 7400 groups from little 250 pg HeLa 50 samples per day (SPD). benchmark multiple analysis strategies, estimate their influence on FDR show that level can easily be maintained 1 %. two-proteome mix, check optimal parameters fold change differences 2 still successfully determined inputs. Eventually, apply our workflow A549 cells yielding 5300 single cell, which allows observation heterogeneity cellular population studying dependencies between cell size cell-cycle phase. Additionally, work-flow enables us distinguish vitro analogs two human blastocyst lineages: naïve pluripotent stem (epiblast) trophectoderm (TE)-like cells. Gene Ontology enriched proteins TE-like harmoniously aligns transcriptomic data, indicating possesses capability biologically relevant these lineages within blastocyst.

Язык: Английский

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Review of electrophysiological models to study membrane potential changes in breast cancer cell transformation and tumor progression

Frontiers in Physiology, Год журнала: 2025, Номер 16

Опубликована: Март 5, 2025

The transformation of normal breast cells into cancerous is a complex process influenced by both genetic and microenvironmental factors. Recent studies highlight the significant role membrane potential (Vm) alterations in this transformation. Cancer typically exhibit depolarized resting (RMP) compared to cells, which correlates with increased cellular activity more aggressive cancer behavior. These RMP Vm changes are associated altered ion channel activity, calcium dynamics, mitochondrial dysfunction, modified gap junction communication, disrupted signaling pathways. Such fluctuations influence key processes progression, including cell proliferation, migration, invasion. Notably, subtypes display frequent pronounced fluctuations. Understanding electrical properties provides new insights their behavior offers therapeutic targets, such as channels regulation. This review synthesizes current research on how various factors modulate proposes an electrophysiological model based experimental clinical data from literature. findings may pave way for novel pharmacological targets clinicians, researchers, pharmacologists treating cancer.

Язык: Английский

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Snail Slime Extracted by a Cruelty Free Method Preserves Viability and Controls Inflammation Occurrence: A Focus on Fibroblasts

Molecules, Год журнала: 2023, Номер 28(3), С. 1222 - 1222

Опубликована: Янв. 26, 2023

Snail slime (SS) is a viscous secretion obtained from different snail species. SS composition variable according to factors such as the extraction method. Even if several papers have been published regarding this topic, molecular mechanisms at base of biological effects remain unexplored. Thus, aim study evaluate capability SS, extracted with cruelty-free Muller method, promote viability and angiogenesis processes and, in parallel, counteract inflammation occurrence on skin cell populations. was administered keratinocytes, macrophages fibroblasts, then viability, through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, cytotoxicity by lactate dehydrogenase (LDH) assay, morphology haematoxylin-eosin staining, gene protein expression real-time polymerase chain reaction (PCR) Western blot, cycle phases flow cytometry, collagen using an enzyme-linked immunosorbent assay (ELISA) were measured. Our results evidence fibroblast trigger recovery activating Erk protein. Moreover, appreciable anti-inflammatory effect due significant reduction cyclooxygenase-2 expression, positive modulation new blood vessel formation demonstrated increased Angiopoietin 1 higher matrix deposition (evidenced augmented amount released I) can be identified. This led us assume that method extracted-SS represents valuable promising natural product suitable for cosmetic care formulations.

Язык: Английский

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Scutellaria baicalensis Georgi and Their Natural Flavonoid Compounds in the Treatment of Ovarian Cancer: A Review

Molecules, Год журнала: 2023, Номер 28(13), С. 5082 - 5082

Опубликована: Июнь 29, 2023

Ovarian cancer (OC) is one of the most common types in women with a high mortality rate, and treatment OC prone to recurrence rates side effects. Scutellaria baicalensis (SB) herbal medicine good anti-cancer activity, several studies have shown that SB its flavonoids some anti-OC properties. This paper elucidated pathogenesis OC, including cell proliferation cycle regulation, invasion metastasis, apoptosis autophagy, drug resistance angiogenesis. The mechanisms flavonoids, wogonin, baicalein, baicalin, Oroxylin A, scutellarein, are revealed, such as wogonin inhibits proliferation, induces apoptosis, increases cytotoxicity drug. Baicalein also vascular endothelial growth factor (VEGF) expression etc. Analyzing their advantages disadvantages treating provides new perspective on role treatment. It serves resource for future research development.

Язык: Английский

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Targeting cardiomyocyte cell cycle regulation in heart failure

Basic Research in Cardiology, Год журнала: 2024, Номер 119(3), С. 349 - 369

Опубликована: Апрель 29, 2024

Heart failure continues to be a significant global health concern, causing substantial morbidity and mortality. The limited ability of the adult heart regenerate has posed challenges in finding effective treatments for cardiac pathologies. While various medications surgical interventions have been used improve function, they are not able address extensive loss functioning cardiomyocytes that occurs during injury. As result, there is growing interest understanding how cell cycle regulated exploring potential stimulating cardiomyocyte proliferation as means promoting regeneration. This review aims provide an overview current knowledge on regulation mechanisms underlying cases failure, while also highlighting established novel therapeutic strategies targeting this area treatment purposes.

Язык: Английский

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