LST-1 is a bifunctional regulator that feeds back on Notch-dependent transcription to regulate C. elegans germline stem cells
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(39)
Опубликована: Сен. 20, 2023
Notch
signaling
regulates
stem
cells
across
animal
phylogeny.
C.
elegans
activates
transcription
of
two
genes,
lst-1
and
sygl-1,
that
encode
potent
regulators
germline
cells.
The
LST-1
protein
in
distinct
ways:
It
promotes
self-renewal
posttranscriptionally
also
restricts
by
a
poorly
understood
mechanism.
Its
promoting
activity
resides
its
N-terminal
region,
while
restricting
C-terminal
region
requires
the
Zn
finger.
Here,
we
report
limits
down-regulating
Notch-dependent
transcription.
We
detect
nucleus,
addition
to
previously
known
cytoplasmic
localization.
lowers
nascent
transcript
levels
at
both
sygl-1
loci
but
not
let-858,
Notch-independent
locus.
key
components
activation
complex,
LAG-1
DNA
binding
intracellular
domain
(NICD).
Genetically,
an
finger
mutant
increases
strength
gain-
loss-of-function
GLP-1/Notch
receptor
mutants.
Biochemically,
co-immunoprecipitates
with
from
nematode
extracts,
suggesting
direct
effect.
is
thus
bifunctional
regulator
coordinates
posttranscriptional
transcriptional
mechanisms
single
protein.
This
bifunctionality
relies
on
bipartite
architecture
bolstered
generation
isoforms,
one
specialized
for
downregulation.
A
conserved
theme
worms
human
coupling
PUF-mediated
RNA
repression
together
feedback
same
Язык: Английский
A Transcription Factor Functional Atlas of Germline Development
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 26, 2024
Abstract
Fertility
requires
the
faithful
proliferation
of
germ
cells
and
their
differentiation
into
gametes.
Controlling
these
cellular
states
demands
precise
timing
expression
gene
networks.
Transcription
factors
(TFs)
play
critical
roles
in
networks
that
influence
cell
development.
There
has,
however,
been
no
functional
analysis
entire
TF
repertoire
controlling
vivo
Here,
we
analyzed
germline
architecture
to
systematically
investigate
function
364
germline-expressed
TFs
Caenorhabditis
elegans
line.
Using
germline-specific
knockdown,
automated
counting,
high-content
nuclei
plasma
membrane
organization,
identify
156
with
discrete
autonomous
functions.
By
identifying
control
cycle,
proliferation,
differentiation,
structure
fertility,
have
created
an
atlas
for
mechanistic
dissection
behavior
gamete
production.
Язык: Английский
A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans
Wei Cao,
Qi Fan,
Gemmarie Amparado
и другие.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 11, 2024
Fertility
requires
the
faithful
proliferation
of
germ
cells
and
their
differentiation
into
gametes.
Controlling
these
cellular
states
demands
precise
timing
expression
gene
networks.
Nucleic
acid
binding
proteins
(NBPs)
play
critical
roles
in
networks
that
influence
cell
development.
There
has,
however,
been
no
functional
analysis
entire
NBP
repertoire
controlling
vivo
Here,
we
analyzed
germline
architecture
to
systematically
investigate
function
364
germline-expressed
NBPs
Caenorhabditis
elegans
line.
Using
germline-specific
knockdown,
automated
counting,
high-content
nuclei
plasma
membrane
organization,
identify
156
with
discrete
autonomous
functions.
By
identifying
control
cycle,
proliferation,
differentiation,
structure
fertility,
have
created
an
atlas
for
mechanistic
dissection
behavior
gamete
production.
Язык: Английский
The PUF RNA-binding protein, FBF-2, maintains stem cells without binding to RNA
Опубликована: Окт. 25, 2024
Abstract
Like
all
canonical
PUF
proteins,
C.
elegans
FBF-2
binds
to
specific
RNAs
via
tripartite
recognition
motifs
(TRMs).
Here
we
report
that
an
mutant
protein
cannot
bind
RNA,
is
nonetheless
biologically
active
and
maintains
stem
cells.
This
unexpected
result
challenges
the
conventional
wisdom
RBPs
must
achieve
biological
activity.
Also
unexpectedly,
interactions
with
partner
proteins
can
compensate
for
loss
of
RNA-binding.
only
loses
activity
when
its
RNA-binding
are
both
defective.
These
findings
highlight
complementary
contributions
protein.
Язык: Английский
Dynamics of Notch-dependent transcriptional bursting in its native context
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2018,
Номер
unknown
Опубликована: Дек. 15, 2018
Transcription
is
well
known
to
be
inherently
stochastic
and
episodic,
but
the
regulation
of
transcriptional
dynamics
not
understood.
Here
we
analyze
how
Notch
signaling
modulates
bursting
during
animal
development.
Our
focus
transcription
in
germline
stem
cells
nematode
C.
elegans.
Using
MS2
system
visualize
nascent
transcripts
live
imaging
record
dynamics,
as
a
function
position
within
intact
animal.
We
find
that
Notch-dependent
activation
indeed
bursty;
wild-type
burst
duration
(ON-time)
rather
than
pauses
between
bursts
(OFF-time)
or
mean
intensity;
mutant
receptor,
which
compromised
for
assembly
into
factor
complex,
primarily
modifies
size
(duration
x
intensity).
To
our
knowledge,
this
work
first
metazoan
by
canonical
pathway
its
native
context.
Язык: Английский
Faculty Opinions recommendation of The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in Caenorhabditis elegans.
Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature,
Год журнала:
2018,
Номер
unknown
Опубликована: Июнь 18, 2018
The
purpose
of
germ
cells
is
to
ensure
the
faithful
transmission
genetic
material
next
generation.To
develop
into
mature
gametes,
must
pass
through
cell
cycle
checkpoints
while
maintaining
totipotency
and
genomic
integrity.How
coordinate
developmental
events
simultaneously
protecting
their
unique
fate
not
well
understood.Here,
we
characterize
a
novel
nuclear
protein,
Oocyte-Excluded
Factor-1
(OEF-1),
with
highly
specific
germline
expression
in
Caenorhabditis
elegans.OEF-1
initially
detected
early
embryogenesis
expressed
nuclei
all
during
larval
stages.In
adults,
OEF-1
abruptly
decreases
just
prior
oocyte
differentiation.In
oef-1
mutants,
progression
accelerated,
resulting
subtle
defects
at
multiple
stages
development.Lastly,
primarily
associated
bodies
germlineexpressed
genes,
as
such
excluded
from
X
chromosome.We
hypothesize
that
may
regulate
rate
development,
providing
insight
how
these
critical
maturation
are
coordinated.
Язык: Английский
Faculty Opinions recommendation of An RNA-Binding Multimer Specifies Nematode Sperm Fate.
Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature,
Год журнала:
2018,
Номер
unknown
Опубликована: Ноя. 22, 2018
FOG-3
is
a
master
regulator
of
sperm
fate
in
Caenorhabditis
elegans
and
homologous
to
Tob/BTG
proteins,
which
mammals
are
monomeric
adaptors
that
recruit
enzymes
RNA
binding
proteins.Here,
we
determine
the
crystal
structure
vitro
demonstrate
forms
dimers
can
multi-merize.The
multimeric
has
basic
surface
potential,
suggestive
nucleic
acid.Consistent
with
prediction,
binds
directly
nearly
1,000
RNAs
nematode
spermatogenic
germ
cells.Most
3
′
UTR,
most
targets
(94%)
oogenic
mRNAs,
even
though
assayed
cells.When
tethered
reporter
mRNA,
represses
its
expression.Together
these
findings
elucidate
molecular
mechanism
specification
reveal
evolution
protein
from
form
acquisition
distinct
mode
mRNA
repression.
Язык: Английский