<b><i>Xenopus</i></b>: An Undervalued Model Organism to Study and Model Human Genetic Disease
Cells Tissues Organs,
Год журнала:
2018,
Номер
205(5-6), С. 303 - 313
Опубликована: Янв. 1, 2018
The
function
of
normal
and
defective
candidate
genes
for
human
genetic
diseases,
which
are
rapidly
being
identified
in
large
numbers
by
geneticists
the
biomedical
community
at
large,
will
be
best
studied
relevant
predictive
model
organisms
that
allow
high-speed
verification,
analysis
underlying
developmental,
cellular
molecular
mechanisms,
establishment
disease
models
to
test
therapeutic
options.
We
describe
discuss
pros
cons
frog
Xenopus,
has
been
extensively
used
uncover
developmental
mechanisms
past,
but
is
underutilized
as
a
model.
argue
Xenopus
complements
more
commonly
mouse
zebrafish
time-
cost-efficient
animal
study
alleles
mechanisms.
Язык: Английский
CRISPR/Cas9 disease models in zebrafish and Xenopus: The genetic renaissance of fish and frogs
Drug Discovery Today Technologies,
Год журнала:
2018,
Номер
28, С. 41 - 52
Опубликована: Июль 31, 2018
Язык: Английский
Genome-editing approaches and applications: a brief review on CRISPR technology and its role in cancer
3 Biotech,
Год журнала:
2021,
Номер
11(3)
Опубликована: Фев. 26, 2021
Язык: Английский
Potential Roles of the Retinoblastoma Protein in Regulating Genome Editing
Frontiers in Cell and Developmental Biology,
Год журнала:
2018,
Номер
6
Опубликована: Июль 31, 2018
Genome
editing
is
an
important
tool
for
modifying
genomic
DNA
through
introducing
insertion
or
deletion
at
specific
locations
of
a
genome.
Recently
CRISPR/Cas9
has
been
widely
employed
to
improve
the
efficiency
genome
editing.
The
Cas9
nuclease
creates
site-specific
double
strand
breaks
(DSBs)
targeted
loci
in
Subsequently,
DSBs
are
repaired
by
two
pathways:
Homologous
Recombination
(HR)
and
Non-Homologous
End-Joining
(NHEJ).
HR
considered
as
"error-free"
because
it
repairs
copying
sequences
from
homologous
template,
while
NHEJ
"error-prone"
there
base
deletions
insertions
breakage
site.
Recently,
RB1,
gene
that
commonly
mutated
retinoblastoma,
reported
affect
repair
efficiencies
NHEJ.
This
review
focuses
on
roles
RB1
repairing
DSBs,
which
have
impacts
precision
consequences
editing,
both
overall
Язык: Английский
RBL1 (p107) functions as tumor suppressor in glioblastoma and small-cell pancreatic neuroendocrine carcinoma
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2019,
Номер
unknown
Опубликована: Янв. 23, 2019
Abstract
Alterations
of
the
retinoblastoma
and/or
p53
signaling
network
are
associated
with
specific
cancers
such
as
high-grade
astrocytoma/glioblastoma,
small
cell
lung
cancer
(SCLC),
choroid
plexus
tumors
and
small-cell
pancreatic
neuroendocrine
carcinoma
(SC-PaNEC).
However,
intricate
functional
compensation
between
RB1
related
pocket
proteins
RBL1/p107
RBL2/p130
in
suppressing
tumorigenesis
remains
poorly
understood.
Here
we
performed
lineage-restricted
parallel
inactivation
rb1
rbl1
by
multiplex
CRISPR/Cas9
genome
editing
true
diploid
Xenopus
tropicalis
to
gain
insight
into
these
vivo
compensatory
mechanisms.
We
show
that
while
is
sufficient
induce
papilloma,
combined
required
drive
SC-PaNEC,
astrocytoma.
Further,
using
a
novel
Li-Fraumeni
syndrome-mimicking
tp53
mutant
X.
line,
demonstrate
increased
malignancy
retinoblastoma-mutant
neural
malignancies
upon
concomitant
.
Interestingly,
although
clinical
SC-PaNEC
samples
characterized
abnormal
expression
or
localization,
current
experimental
models,
status
had
little
effect
on
establishment
growth
but
may
rather
be
essential
for
maintaining
chromosomal
stability.
SCLC
was
only
rarely
observed
our
set-up,
indicating
requirement
additional
alternative
oncogenic
insults.
In
conclusion,
used
delineate
tumor
suppressor
properties
Rbl1
generate
new
insights
within
protein
family
cancers.
Язык: Английский