Eco-Friendly Biobleaching of Lignocellulosic Biomass for Pulp and Paper Industry

Опубликована: Янв. 1, 2025

Язык: Английский

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Metabolic Engineering of Fungi for Enhanced Production of Lignocellulolytic Enzymes

Опубликована: Янв. 1, 2025

Язык: Английский

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A CRISPR Cas12a/Cpf1 strategy to facilitate robust multiplex gene editing in Aspergillus Niger

Fungal Biology and Biotechnology, Год журнала: 2025, Номер 12(1)

Опубликована: Апрель 25, 2025

CRISPR technologies have revolutionized strain engineering of Aspergillus species, and drastically increased the ease speed at which genomic modifications can be performed. One advantages is possibility rapid using multiplex experiments. This achieved by a set different guiding RNA molecules (gRNA) to target multiple loci in same experiment. Two major challenges such experiments are firstly, delivery guides simultaneously, secondly, ensuring that each locus cut efficiently nuclease. The nuclease Cas12a, also known as Cpf1, presents unique advantage bypass this challenge. Specifically, unlike Cas9, Cpf1 able release several gRNAs from common precursor molecule through its own RNase activity, eliminating need for elements ribozymes or tRNA machinery gRNA maturation. feature sets stage much more straightforward construction vectors many gRNAs, turn allows targeted increase odds successfully inducing break DNA. Here we present toolbox used assemble plasmids containing expression cassette, express multi precursor. processed via activity produce functional vivo. Using our setup, constructed deliver up ten gRNAs. In addition, show three simultaneous deletions introduced robustly niger targeting gene with without prior validation use genomically integrated selection markers. study established an efficient system CRISPR-Cpf1 large number genome editing species. Our strategy specific performed time frame less than two weeks, envision will cell factory efforts significantly.

Язык: Английский

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Penicillium chrysogenum as a fungal factory for feruloyl esterases

Applied Microbiology and Biotechnology, Год журнала: 2023, Номер 107(2-3), С. 691 - 717

Опубликована: Янв. 3, 2023

Язык: Английский

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Two-step biocatalytic conversion of post-consumer polyethylene terephthalate into value-added products facilitated by genetic and bioprocess engineering

Bioresource Technology, Год журнала: 2024, Номер unknown, С. 131837 - 131837

Опубликована: Ноя. 1, 2024

Язык: Английский

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