Long-Term Prognostic Factors in Patients With Antineutrophil Cytoplasmic Antibody-Associated Vasculitis: A 15-Year Multicenter Retrospective Study

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Июнь 30, 2022

Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a multisystem autoimmune disease with small-vessel involvement. In AAV, microscopic polyangiitis (MPA) and granulomatosis (GPA) are major clinicopathologic variants. addition, myeloperoxidase (MPO) proteinase 3 (PR3) target antigens. The objective of the study was to explore predictive factors for long-term survival in AAV patients. A multicenter retrospective carried out on 407 patients between 2005 2020. Clinical parameters were obtained from laboratory tests including ANCA types, antinuclear (ANA), extractable nuclear antigen (ENA), anti-streptolysin O (ASO), glomerular filtration rate (GFR), examinations blood routine, liver function, renal immunity, etc. data clinical collected electronic medical records (EMRs), patient acquired through regular follow-up. association overall (OS) along 3-year 5-year rates analyzed, nomogram as model established according analysis results. present study, 336 (82.6%) 46 (11.3%) diagnosed MPA GPA, respectively. mean median OS all 2,285 2,290 days, 1-year, 3-year, 5-year, 10-year cumulative 84.2%, 76.3%, 57.2%, 32.4%, Univariate multivariate analyses indicated that independent prognostic included age, pathological categories (MPA, other types), serum types (negative or positive MPO and/or PR3), ANA, ASO, GFR, lymphocyte, neutrophil-to-lymphocyte ratio (NLR), C-reactive protein (CRP), these except ASO used construct nomogram. had C-index 0.721 (95% CI 0.676-0.766). calibration curves showed predicted values generally consistent practical observed values, decision curve (DCA) further demonstrated practicability accuracy model. Laboratory at diagnosis have great significance prediction

Язык: Английский

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Sphingosine 1-phosphate has a negative effect on RBC storage quality

Blood Advances, Год журнала: 2022, Номер 7(8), С. 1379 - 1393

Опубликована: Дек. 5, 2022

Blood storage promotes the rapid depletion of red blood cell (RBC) high-energy adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (DPG), which are critical regulators erythrocyte physiology function, as well oxygen kinetics posttransfusion survival. Sphingosine-1-phosphate (S1P) fluxes through glycolysis. We hypothesized that S1P supplementation to stored RBC units would improve energy metabolism recovery. quantified in 1929 samples (n = 643, days 10, 23, 42) from REDS Omics study. then supplemented human murine RBCs good storer (C57BL6/J) poor strains (FVB) with (1, 5, 10 μM) before measurements Similar experiments were repeated for mice genetic ablation biosynthetic pathway (sphingosine kinase 1 [Sphk1] knockout [KO]). Sample analyses included metabolomics at steady state, tracing 1,2,3-13C3-glucose, proteomics, analysis end-of-storage recovery, under normoxic hypoxic conditions. Storage promoted decreases levels, highest donated by female or older donors. Supplementation boosted steady-state levels glycolytic metabolites fluxes, ie generation ATP DPG, expense pentose phosphate pathway. Lower recovery was observed upon supplementation. All these phenomena reversed Sphk1 KO storage. is a positive regulator negative antioxidant RBCs, resulting lower recoveries models.

Язык: Английский

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CRISPR/Cas-based gene editing in therapeutic strategies for beta-thalassemia

Human Genetics, Год журнала: 2023, Номер 142(12), С. 1677 - 1703

Опубликована: Окт. 25, 2023

Язык: Английский

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Hemoglobin-to-red blood cell distribution width ratio is negatively associated with stroke: a cross-sectional study from NHANES

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 15, 2024

Numerous studies have suggested that the hemoglobin-to-red blood cell distribution width ratio (HRR) is associated with onset, progression, and prognosis of various diseases. However, to best our knowledge, no research has conducted statistical analyses determine association between HRR stroke. This cross-sectional study was among adults complete data on hemoglobin-red stroke from 2005–2018 National Health Nutrition Examination Survey (NHANES). calculated by dividing hemoglobin (HGB) in grams per deciliter (g/dL) red (RDW). Weighted multivariable logistic regression generalized additive models were employed investigate independent nonlinear relationships Threshold effects assessed using two-piece linear models. Additionally, subgroup interaction tests performed. A total 36,215 participants included, 2.92% classified as patients. The prevalence decreased across increasing tertiles (Q1: 5.07%; Q2: 2.63%; Q3: 1.69%; P < 0.0001). negative observed both unadjusted adjusted In Model III, each one-unit increase a 58% reduction likelihood (Model III: OR = 0.42, 95% CI: 0.29–0.63). Subgroup revealed BMI-dependent (P 0.05), only BMI 25 25–30, but not those > 30. Using model, threshold effect identified at 30 (K 1.16). To left this breakpoint, there (OR 0.55, 0.27–0.97), whereas such detected right breakpoint 6.49, 0.75–56.11). negatively stroke, lower risk individuals higher levels.

Язык: Английский

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The metabolic characteristic of decidual immune cells and their unique properties in pregnancy loss*

Immunological Reviews, Год журнала: 2022, Номер 308(1), С. 168 - 186

Опубликована: Май 18, 2022

Maternal tolerance to semi- or fully allograft conceptus is a prerequisite for the maintenance of pregnancy. Once this homeostasis disrupted, it may result in pregnancy loss. As potential approach prevent loss, targeting decidual immune cells (DICs) at maternal-fetal interface has been suggested. Although phenotypic features and functions DIC have extensively profiled, regulatory pathways unique immunological adaption yet be elucidated. In recent years, pivotal mechanism highlighted area immunometabolism, by which changes intracellular metabolic interaction with adjacent metabolites microenvironment can alter their phenotypes function. More inspiringly, manipulation profiling provides novel avenue prevention treatment Herein, review highlights major programs (specifically, glycolysis, ATP-adenosine metabolism, lysophosphatidic acid amino metabolism) multiple (including NK cells, macrophages, T cells) integrations normal Importantly, perspective help provide therapeutic strategy reducing loss via interplay.

Язык: Английский

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