FEMS Microbiology Ecology,
Год журнала:
2022,
Номер
98(8)
Опубликована: Июнь 11, 2022
Abstract
Organohalide
respiration
(OHR),
catalysed
by
reductive
dehalogenases
(RDases),
plays
an
important
role
in
halogen
cycling.
Natural
organohalides
and
putative
RDase-encoding
genes
have
been
reported
Aarhus
Bay
sediments,
however,
OHR
has
not
experimentally
verified.
Here
we
show
that
sediments
of
can
dehalogenate
a
range
organohalides,
different
differentially
affected
microbial
community
compositions.
PCE-dechlorinating
cultures
were
further
examined
16S
rRNA
gene-targeted
quantitative
PCR
amplicon
sequencing.
Known
organohalide-respiring
bacteria
(OHRB)
including
Dehalococcoides,
Dehalobacter
Desulfitobacterium
decreased
abundance
during
transfers
serial
dilutions,
suggesting
the
importance
yet
uncharacterized
OHRB
these
cultures.
Switching
from
PCE
to
2,6-DBP
led
its
complete
debromination
phenol
with
without
sulfate.
debrominating
differed
composition
Desulfobacterota
genera
recently
verified
include
OHRB,
Desulfovibrio
Desulfuromusa,
enriched
all
microcosms,
whereas
Halodesulfovibrio
was
only
Hydrogen
methane
detected
likely
served
as
electron
donor
for
methanogenesis.
This
study
shows
occur
marine
environments
mediated
unknown
their
natural
Abstract
The
European
Commission
asked
EFSA
to
update
its
2012
risk
assessment
on
brominated
phenols
and
their
derivatives
in
food,
focusing
five
bromophenols
one
derivative:
2,4,6‐tribromophenol
(2,4,6‐TBP),
2,4‐dibromophenol
(2,4‐DBP),
4‐bromophenol
(4‐BP),
2,6‐dibromophenol
(2,6‐DBP),
tetrabrominated
bisphenol
S
(TBBPS),
tetrabromobisphenol
bismethyl
ether
(TBBPS‐BME).
Based
the
overall
evidence,
CONTAM
Panel
considered
vivo
genotoxicity
of
2,4,6‐TBP
be
unlikely.
Effects
liver
kidney
were
as
critical
effects
(2,4,6‐TBP)
studies
rats.
A
BMDL10
353
mg/kg
body
weight
(bw)
per
day
for
papillary
necrosis
male
rats
was
identified
selected
reference
point
characterisation.
derivation
a
health‐based
guidance
value
not
appropriate
due
major
limitations
toxicological
database.
Instead,
margin
exposure
(MOE)
approach
applied
assess
possible
health
concerns.
Around
78,200
analytical
results
food
used
estimate
dietary
population.
Considering
resulting
MOE
values,
all
far
above
an
6000
that
does
raise
concern,
accounting
uncertainties
affecting
hazard
assessments,
concluded
with
at
least
95%
probability
current
concern.
Due
lack
occurrence
data,
no
could
performed
breastfed
or
formula‐fed
infants.
No
characterisation
any
other
included
assessment,
data
both
toxicity
occurrence.
The Journal of Organic Chemistry,
Год журнала:
2012,
Номер
77(9), С. 4198 - 4208
Опубликована: Апрель 9, 2012
Five
new
vinylchlorine-containing
metabolites,
the
lipoamides
janthielamide
A
and
kimbeamides
A-C
ketide-extended
pyranone
kimbelactone
A,
have
been
isolated
from
collections
of
marine
cyanobacteria
made
in
Curaçao
Papua
New
Guinea.
Both
kimbeamide
exhibited
moderate
sodium
channel
blocking
activity
murine
Neuro-2a
cells.
Consistent
with
this
activity,
was
also
found
to
antagonize
veratridine-induced
influx
cerebrocortical
neurons.
These
represent
newest
additions
a
relatively
rare
family
cyanobacterial-derived
structural
class
compounds
exhibiting
neuromodulatory
activities
cyanobacteria.
The Journal of Organic Chemistry,
Год журнала:
2016,
Номер
81(21), С. 10411 - 10423
Опубликована: Авг. 24, 2016
An
investigation
into
the
use
of
Lewis
base
catalysis
for
enantioselective
chlorolactonization
1,2-disubstituted
alkenoic
acids
is
described.
Two
mechanistically
distinct
reaction
pathways
catalytic
have
been
identified.
Mechanistic
revealed
that
tertiary
amines
predominately
operate
as
Brønsted
rather
than
bases.
potential
modes
activation
identified
involve
donation
electron
density
carboxylate
to
C═C
bond
well
hydrogen
bonding
chlorinating
agent.
Sulfur-
and
selenium-based
additives
under
catalysis;
however,
due
instability
intermediate
benzylic
chloriranium
ion,
suffers
from
low
chemo-,
diastereo-,
enantioselectivities.
Independent
generation
ion
shows
it
in
equilibrium
with
an
open
cation,
which
leads
specificities
nucleophilic
capture
intermediate.
Biomedicines,
Год журнала:
2023,
Номер
11(10), С. 2698 - 2698
Опубликована: Окт. 3, 2023
Heteroatom
steroids,
a
diverse
class
of
organic
compounds,
have
attracted
significant
attention
in
the
field
medicinal
chemistry
and
drug
discovery.
The
biological
profiles
heteroatom
steroids
are
considerable
interest
to
chemists,
biologists,
pharmacologists,
pharmaceutical
industry.
These
compounds
shown
promise
as
potential
therapeutic
agents
treatment
various
diseases,
such
cancer,
infectious
cardiovascular
disorders,
neurodegenerative
conditions.
Moreover,
incorporation
heteroatoms
has
led
development
targeted
delivery
systems,
prodrugs,
other
innovative
approaches.
represent
fascinating
area
research,
bridging
fields
chemistry,
pharmacology.
exploration
their
chemical
diversity
activities
holds
for
discovery
novel
candidates
more
effective
treatments.
Journal of Natural Products,
Год журнала:
2024,
Номер
87(2), С. 217 - 227
Опубликована: Янв. 19, 2024
The
urgent
need
for
new
classes
of
orally
available,
safe,
and
effective
antivirals─covering
a
breadth
emerging
viruses─is
evidenced
by
the
loss
life
economic
challenges
created
HIV-1
SARS-CoV-2
pandemics.
As
frontline
interventions,
small-molecule
antivirals
can
be
deployed
prophylactically
or
postinfection
to
control
initial
spread
outbreaks
reducing
transmissibility
symptom
severity.
Natural
products
have
an
impressive
track
record
success
as
prototypic
continue
provide
drugs
through
synthesis,
medicinal
chemistry,
optimization
decades
after
discovery.
Here,
we
demonstrate
approach
using
computational
analysis
typically
used
rational
drug
design
identify
develop
natural
product-inspired
antivirals.
This
was
done
with
goal
identifying
product
prototypes
aid
effort
progressing
toward
effective,
affordable
broad-spectrum
inhibitors
Betacoronavirus
replication
targeting
highly
conserved
RNA
2′-O-methyltransferase
(2′-O-MTase).
Machaeriols
RS-1
(7)
RS-2
(8)
were
identified
previously
outlined
informatics
first
screen
prototypes,
followed
in
silico-guided
synthesis.
Both
molecules
are
based
on
rare
group.
machaeriols
(3–6),
isolated
from
genus
Machaerium,
endemic
Amazonia,
inhibited
2′-O-MTase
more
potently
than
positive
control,
Sinefungin
(2),
silico
modeling
suggests
distinct
molecular
interactions.
report
highlights
potential
computationally
driven
screening
leverage
libraries
improve
efficiency
isolation
synthetic
analog
development.
The Journal of Adhesion,
Год журнала:
2009,
Номер
85(2-3), С. 126 - 138
Опубликована: Апрель 28, 2009
The
sandcastle
worm
Phragmatopoma
californica,
a
marine
polychaete,
constructs
tube-like
shelter
by
cementing
together
sand
grains
using
glue
secreted
from
the
building
organ
in
its
thorax.
is
mixture
of
post-translationally
modified
proteins,
notably
cement
proteins
Pc-1
and
Pc-2
with
amino
acid,
3,4-dihydroxyphenyl-L-alanine
(DOPA).
Significant
amounts
halogenated
derivative
DOPA
were
isolated
following
partial
acid
hydrolysis
capture
catecholic
acids
phenylboronate
affinity
chromatography.
Analysis
tandem
mass
spectrometry
(1)H
NMR
indicates
to
be
2-chloro-4,
5-dihydroxyphenyl-L-alanine.
potential
roles
2-chloro-DOPA
chemical
defense
underwater
adhesion
are
considered.
Microbial Biotechnology,
Год журнала:
2024,
Номер
17(8)
Опубликована: Июль 29, 2024
Abstract
Organohalides
are
widespread
pollutants
that
pose
significant
environmental
hazards
due
to
their
high
degree
of
halogenation
and
elevated
redox
potentials,
making
them
resistant
natural
attenuation.
Traditional
bioremediation
approaches,
primarily
relying
on
bioaugmentation
biostimulation,
often
fall
short
achieving
complete
detoxification.
Furthermore,
the
emergence
complex
halogenated
pollutants,
such
as
per‐
polyfluoroalkyl
substances
(PFASs),
further
complicates
remediation
efforts.
Therefore,
there
is
a
pressing
need
reconsider
novel
approaches
for
more
efficient
these
recalcitrant
pollutants.
This
review
proposes
redox‐potential‐mediated
hybrid
bioprocesses,
tailored
physicochemical
properties
contexts,
achieve
detoxification
organohalides.
The
possible
scenarios
proposed
discussed.
In
anaerobic
environments,
sediment
groundwater,
microbial
reductive
dehalogenation
coupled
with
fermentation
methanogenesis
can
convert
organohalides
into
carbon
dioxide
methane.
environments
anaerobic‐aerobic
alternation,
paddy
soil
wetlands,
synergistic
process
involving
reduction
oxidation
facilitate
mineralization
highly
organic
compounds.
Future
research
should
focus
in‐depth
exploration
consortia,
application
ecological
principles‐guided
strategies,
development
bioinspired‐designed
techniques.
paper
contributes
academic
discourse
by
proposing
innovative
strategies
complexities
organohalide
pollution.
The Journal of Organic Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 20, 2025
Steroids
have
consistently
drawn
scientific
attention
due
to
their
diverse
structures
and
exceptional
pharmacological
activities.
However,
halogenated
natural
steroids
remain
relatively
rare,
especially
for
those
derived
from
plants.
In
this
study,
two
chlorinated
C19
hocarnoids
A
(1)
B
(2)
were
identified
the
folk
medicinal
plant
Hoya
carnosa,
representing
first
examples
of
C-16
steroids.
Semisynthesis
compounds
1
2
was
completed
in
4
5
linear
steps
with
28.2%
25.3%
overall
yields
through
a
modified
approach,
corroborating
further
structures.
total
collection
11
steroids,
including
intermediates
obtained
during
synthesis,
evaluated
anti-inflammatory
activity
against
lipopolysaccharide
(LPS)-induced
inflammation-related
RAW
264.7
macrophages.
Among
them,
product
hocarnoid
displayed
strongest
an
IC50
value
3.62
±
0.08
μM,
which
also
attenuated
mRNA
levels
proinflammatory
cytokines
IL-1β,
IL-6,
IL-10,
TNF-α.
Abstract
Background
Industrial
waste,
agricultural
runoff
and
untreated
sewage
contaminate
the
Nile,
leaving
a
toxic
legacy
in
its
sediments.
Organohalides-polluted
sediment
particular
poses
serious
public
health
risks
detrimental
effects
on
aquatic
life.
Sediment
microbiomes
may
harbor
bacterial
strains
that
could
be
utilized
bioremediation
of
such
pollutants.
Material
methods
Two
from
polluted
River
Nile
sediments
were
analyzed
by
using
16S
rRNA
gene
amplicon
sequencing.
In
addition,
PICRUSt
analysis
based
data
was
used
to
explore
organohalide
respiring
bacteria
(OHRB)
genera
their
corresponding
respiration
(OHR)
activity.
Microcosm
studies
performed
validate
potential
for
dechlorination
activity
sediment.
Dechlorination
parent
chloroethenes
into
daughter
end
product
detected
gas
chromatography
coupled
with
flame
ionization
detection
analysis.
Results
Analysis
sequences
EZ-biocloud
server
identified
Proteobacteria
as
dominant
phylum
both
microbiomes,
Bacteroidetes
Chloroflexi
prevalent
RNS1
Chlorobi
RNS2
PCR
analyses
several
OHRB
genera,
including
Dehalococcoides
,
Dehalogenimonas
Desulfomonile
Desulfovibrio
Geobacter
suggesting
OHR
Further
evidence
provided
functional
prediction
analysis,
which
suggested
presence
reductive
dehalogenases
biomarkers
associated
samples.
Specifically,
predicted
genes
tetrachloroethene
dehalogenase
3-chloro-4-hydroxyphenylacetate
dehalogenase,
previously
linked
OHR.
confirmed
dichloroethene.
Conclusion
This
study
demonstrates
industrialized
area
harbors
distinct
enclosing
various
providing
substantial
It
also
provides