FEMS Microbiology Ecology,
Год журнала:
2022,
Номер
98(8)
Опубликована: Июнь 11, 2022
Abstract
Organohalide
respiration
(OHR),
catalysed
by
reductive
dehalogenases
(RDases),
plays
an
important
role
in
halogen
cycling.
Natural
organohalides
and
putative
RDase-encoding
genes
have
been
reported
Aarhus
Bay
sediments,
however,
OHR
has
not
experimentally
verified.
Here
we
show
that
sediments
of
can
dehalogenate
a
range
organohalides,
different
differentially
affected
microbial
community
compositions.
PCE-dechlorinating
cultures
were
further
examined
16S
rRNA
gene-targeted
quantitative
PCR
amplicon
sequencing.
Known
organohalide-respiring
bacteria
(OHRB)
including
Dehalococcoides,
Dehalobacter
Desulfitobacterium
decreased
abundance
during
transfers
serial
dilutions,
suggesting
the
importance
yet
uncharacterized
OHRB
these
cultures.
Switching
from
PCE
to
2,6-DBP
led
its
complete
debromination
phenol
with
without
sulfate.
debrominating
differed
composition
Desulfobacterota
genera
recently
verified
include
OHRB,
Desulfovibrio
Desulfuromusa,
enriched
all
microcosms,
whereas
Halodesulfovibrio
was
only
Hydrogen
methane
detected
likely
served
as
electron
donor
for
methanogenesis.
This
study
shows
occur
marine
environments
mediated
unknown
their
natural
Genome Announcements,
Год журнала:
2017,
Номер
5(37)
Опубликована: Сен. 14, 2017
ABSTRACT
Dehalobacterium
formicoaceticum
utilizes
dichloromethane
as
the
sole
energy
source
in
defined
anoxic
bicarbonate-buffered
mineral
salt
medium.
The
products
are
formate,
acetate,
inorganic
chloride,
and
biomass.
bacterium’s
genome
was
sequenced
using
PacBio,
assembled,
annotated.
complete
consists
of
one
3.77-Mb
circular
chromosome
harboring
3,935
predicted
protein-encoding
genes.
Beilstein Journal of Organic Chemistry,
Год журнала:
2017,
Номер
13, С. 1168 - 1173
Опубликована: Июнь 16, 2017
The
in
vitro
biochemical
characterization
revealed
that
iron/2-oxoglutarate
(Fe/2OG)-dependent
aliphatic
halogenase
WelO5*
Hapalosiphon
welwitschii
IC-52-3
has
an
enhanced
substrate
specificity
towards
12-epi-hapalindole
C
(1)
comparison
to
WelO5
H.
UTEX
B1830.
This
allowed
us
define
the
origin
of
varied
chlorinated
versus
dechlorinated
alkaloid
structural
diversity
between
two
welwitindolinone
producers.
Furthermore,
this
study,
along
with
recent
AmbO5
protein,
collectively
confirmed
presence
a
signature
sequence
motif
C-terminus
newly
discovered
enzyme
family
confers
promiscuity
and
specificity.
These
observations
may
guide
rational
engineering
evolution
these
proteins
for
biocatalyst
application.
Environment & Health,
Год журнала:
2024,
Номер
2(4), С. 233 - 242
Опубликована: Фев. 19, 2024
Halogenated
natural
products
(HNPs)
are
considered
to
be
emerging
contaminants
whose
environmental
distribution
and
fate
only
incompletely
known.
Therefore,
several
persistent
bioaccumulative
HNP
groups,
together
with
man-made
polychlorinated
biphenyls
(PCBs)
polybrominated
diphenyl
ethers
(PBDEs),
were
quantified
in
the
blubber
of
nine
sperm
whales
(Physeter
macrocephalus)
stranded
on
coast
Mediterranean
Sea
Italy.
The
naturally
occurring
hexahydroxanthene
derivatives
(PBHDs;
sum
TetraBHD
TriBHD)
most
prominent
substance
class
up
77,000
ng/g
blubber.
mean
PBHD
content
(35,800
blubber)
even
exceeded
one
PCBs
(28,400
blubber),
although
region
is
known
highly
contaminated
contaminants.
Based
values,
Q1
∼
PBDEs
>
MeO-BDEs
2,2′-diMeO-BB
80
other
HNPs
followed
decreasing
amounts.
All
samples
contained
an
abundant
compound
molecular
formula
(C16H19Br3O2)
was
verified
using
high-resolution
mass
spectrometry.
plausible
matching
isomer
(2S,4′S,9R,9′S)-2,7-dibromo-4′-bromomethyl-1,1-dimethyl-2,3,4,4′,9,9′-9,9′-hexahydro-1H-xanthen-9-ol
(OH-TriBHD),
a
hydroxylated
secondary
metabolite
previously
detected
TriBHD
sponge
producer
PBHDs.
estimated
amount
presumed
OH-TriBHD
3000
blubber,
which
unexpectedly
high
for
compounds
lipids
marine
mammals.
Frontiers in Chemistry,
Год журнала:
2014,
Номер
2
Опубликована: Ноя. 11, 2014
The
halogenases
are
a
group
of
enzymes
that
have
only
come
to
the
fore
over
last
ten
years
thanks
discovery
and
characterization
several
novel
representatives.
They
re-vealed
fascinating
variety
distinct
chemical
mechanisms
nature
utilizes
activate
introduce
halogens
into
organic
substrates.
Computational
studies
using
range
approaches
already
elucidated
many
details
these
enzymes,
often
in
synergistic
combination
with
experiment.
This
Review
summarizes
main
insights
gained
from
stud-ies.
It
also
seeks
identify
open
questions
amenable
computational
investigations.
discussed
herein
serve
illustrate
some
limitations
current
computa-tional
challenges
encountered
mechanistic
enzymology.
FEMS Microbiology Ecology,
Год журнала:
2022,
Номер
98(8)
Опубликована: Июнь 11, 2022
Abstract
Organohalide
respiration
(OHR),
catalysed
by
reductive
dehalogenases
(RDases),
plays
an
important
role
in
halogen
cycling.
Natural
organohalides
and
putative
RDase-encoding
genes
have
been
reported
Aarhus
Bay
sediments,
however,
OHR
has
not
experimentally
verified.
Here
we
show
that
sediments
of
can
dehalogenate
a
range
organohalides,
different
differentially
affected
microbial
community
compositions.
PCE-dechlorinating
cultures
were
further
examined
16S
rRNA
gene-targeted
quantitative
PCR
amplicon
sequencing.
Known
organohalide-respiring
bacteria
(OHRB)
including
Dehalococcoides,
Dehalobacter
Desulfitobacterium
decreased
abundance
during
transfers
serial
dilutions,
suggesting
the
importance
yet
uncharacterized
OHRB
these
cultures.
Switching
from
PCE
to
2,6-DBP
led
its
complete
debromination
phenol
with
without
sulfate.
debrominating
differed
composition
Desulfobacterota
genera
recently
verified
include
OHRB,
Desulfovibrio
Desulfuromusa,
enriched
all
microcosms,
whereas
Halodesulfovibrio
was
only
Hydrogen
methane
detected
likely
served
as
electron
donor
for
methanogenesis.
This
study
shows
occur
marine
environments
mediated
unknown
their
natural
