CRISPR/Cas9 and Anther Culture for Precision Double Haploid Line Production in Controlled Environments DOI Open Access
Avinash Sharma, Himanshu Pandey, Varucha Misra

и другие.

Plant Breeding, Год журнала: 2024, Номер unknown

Опубликована: Окт. 9, 2024

ABSTRACT The development of mapping populations and quantitative trait loci (QTL) analysis face constraints, in crops exhibiting male sterility self‐incompatibility under field conditions. Addressing these challenges requires the integration advanced techniques, including temporal alteration or excision centromere histone H3 (CENH3) protein use gene editing tools such as MATRILINEAL (MTL) knockout. Specifically, this can be achieved through Cas9/gRNA‐mediated mutagenesis Cas9/gRNA‐driven promoter expression systems. These technologies offer efficient means to advance QTL sterile self‐incompatible within controlled ecosystems. doubled haploid (DH) population, traditionally requiring 3 years generation time via anther culture method, now expedited 2–3 using techniques environmental Notably, DH efficiently generated various crops, rice, wheat, maize, barley oats by leveraging tools. Among tools, novel approach CENH3 alteration/excision emerges highly compared MTL knockout mutation Cas9/gRNA expression. However, further investigation is warranted optimise regeneration double enhance

Язык: Английский

Controlled Environment Ecosystem: A Cutting-Edge Technology in Speed Breeding DOI Creative Commons
Avinash Sharma,

Mainu Hazarika,

Punabati Heisnam

и другие.

ACS Omega, Год журнала: 2024, Номер 9(27), С. 29114 - 29138

Опубликована: Июнь 26, 2024

The controlled environment ecosystem is a meticulously designed plant growing chamber utilized for cultivating biofortified crops and microgreens, addressing hidden hunger malnutrition prevalent in the population. integration of speed breeding within such environments effectively eradicates morphological disruptions encountered traditional methods as inbreeding depression, male sterility, self-incompatibility, embryo abortion, other unsuccessful attempts. In contrast to unpredictable climate conditions that often prolong cycles 10-15 years 4-5 transgenic open ecosystems, techniques expedite achievement objectives F1-F6 generations 2-3 under conditions. comparison, may take 5-10 population line creation, 3-5 field trials, 1-2 variety release. effectiveness trait improvement development varies across different crops, requiring approximately 4 rice groundnut, 5 soybean, pea, oat, 6 sorghum,

Язык: Английский

Процитировано

3

Advances in Seed Production Technology in Field Crops DOI

S. K. Chakrabarty,

Debashis Paul, Sampa Saha

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Photoperiod-mediated rapid generation advancement in soybean (Glycine max (L.) Merr.) DOI

Ambika Rajendran,

Ayyagari Ramlal, Dhandapani Raju

и другие.

Photosynthesis Research, Год журнала: 2025, Номер 163(2)

Опубликована: Март 19, 2025

Язык: Английский

Процитировано

0

CRISPR/Cas9 and Anther Culture for Precision Double Haploid Line Production in Controlled Environments DOI Open Access
Avinash Sharma, Himanshu Pandey, Varucha Misra

и другие.

Plant Breeding, Год журнала: 2024, Номер unknown

Опубликована: Окт. 9, 2024

ABSTRACT The development of mapping populations and quantitative trait loci (QTL) analysis face constraints, in crops exhibiting male sterility self‐incompatibility under field conditions. Addressing these challenges requires the integration advanced techniques, including temporal alteration or excision centromere histone H3 (CENH3) protein use gene editing tools such as MATRILINEAL (MTL) knockout. Specifically, this can be achieved through Cas9/gRNA‐mediated mutagenesis Cas9/gRNA‐driven promoter expression systems. These technologies offer efficient means to advance QTL sterile self‐incompatible within controlled ecosystems. doubled haploid (DH) population, traditionally requiring 3 years generation time via anther culture method, now expedited 2–3 using techniques environmental Notably, DH efficiently generated various crops, rice, wheat, maize, barley oats by leveraging tools. Among tools, novel approach CENH3 alteration/excision emerges highly compared MTL knockout mutation Cas9/gRNA expression. However, further investigation is warranted optimise regeneration double enhance

Язык: Английский

Процитировано

0