Estudo da fotocitotoxicidade dos corantes ciânicos com dois cromóforos em culturas de células neoplásicas DOI Creative Commons

Luciana Sayuri Murakami

Опубликована: Окт. 29, 2009

Os corantes ciânicos com dois cromóforos possuem características espectrais e energéticas vantajosas para aplicação em Terapia Fotodinâmica (TFD) do câncer.Entretanto, sua fotoatividade contra neoplasias não foi ainda estudada nem in vivo vitro.Nesta tese apresentamos os resultados dos estudos vitro mecanismos da fotocitotoxicidade (BCD α ) ângulos entre = 180 0 , 150 90 células neoplásicas, a finalidade de avaliar potencialidade BCD como fotossensibilizadores (FS) TFD.Os foram realizados comparação o fotossensibilizador Photogem®,que já está sendo aplicado TFD.Foram estudados efeito fototóxico, distribuição intracelular contribuição apoptose necrose na morte celular induzida por ele.Além disso, realizada busca formulação farmacêutica adequada tópica .Nos utilizadas as neoplásicas melanoma murino B16F10, humano C8161, adenocarcinoma colo retal HT29, leucemia T (

2D and 3D cell cultures – a comparison of different types of cancer cell cultures DOI Creative Commons

Marta Kapałczyńska,

Tomasz Kolenda,

Weronika Przybyła

и другие.

Archives of Medical Science, Год журнала: 2016, Номер unknown

Опубликована: Янв. 1, 2016

Cell culture is a widely used in vitro tool for improving our understanding of cell biology, tissue morphology, and mechanisms diseases, drug action, protein production the development engineering. Most research regarding cancer biology based on experiments using two-dimensional (2D) cultures vitro. However, 2D have many limitations, such as disturbance interactions between cellular extracellular environments, changes polarity, method division. These disadvantages led to creation models which are more closely able mimic conditions vivo. One three-dimensional (3D). Optimisation may allow better facilitate study biomarkers targeting therapies. In this review, we compare 3D well different versions cultures.

Язык: Английский

Процитировано

1229

New Insights into Cell Culture Technology DOI

Sivakumar Joghi Thatha Gowder

InTech eBooks, Год журнала: 2017, Номер unknown

Опубликована: Май 10, 2017

The book "New Insights into Cell Culture Technology" focuses on many advanced methods and techniques concerned with cell culture. contributing authors have discussed various developments in culture methods, the application of insect cells for efficient production heterologous proteins, expansion human mesenchymal stromal different clinical applications, remote sensing experiments concepts development bioprocess, continuous retroviral pseudotype vectors, oncolytic measles virus vectors cancer therapy. This is an original contribution experts from parts globe, in-depth information will be a significant resource students, scientists, physicians who are directly dealing cells.

["Culture" essential life also cell. - Sivakumar Gowder]

Язык: Английский

Процитировано

60

Current approaches and techniques of 3D cell culture systems: a review DOI Open Access
Lucia Zuščíková,

Hana Greifová,

Denis Bažány

и другие.

Archives of Ecotoxicology, Год журнала: 2024, Номер 6(1), С. 22 - 27

Опубликована: Июнь 29, 2024

Presently, the majority of cells are cultivated by two-dimensional (2D) methods; however, latest and enhanced procedures employing three-dimensional (3D) cell culturing techniques provide strong indications that significantly more sophisticated studies can be carried out, providing invaluable insights. Recent years have seen a rapid development 3D cultures since grown in static environment on flat substrate far from reaching an vivo condition. Currently, scientists gradually realising vitro shape, structure, physiological activities may achieved. As resolution, matrix-like framework for attachment, proliferation, differentiation, communication both dynamic culture conditions is what carriers come to offer. Different mechanical stimulations closely resemble genuine microenvironment could main function systems. Current developments been presented this review, along with discussion their benefits drawbacks when compared conventional 2D growth under settings.

Язык: Английский

Процитировано

1

Porcine parvovirus as a contaminant in cell cultures and laboratory supplies DOI
Tatiana Flávia Pinheiro de Oliveira, Antônio Augusto Fonseca Júnior, Marcelo Fernandes Camargos

и другие.

Biologicals, Год журнала: 2016, Номер 44(2), С. 53 - 59

Опубликована: Янв. 19, 2016

Язык: Английский

Процитировано

7

Preclinical models in oncological pharmacology: limits and advantages DOI Open Access
Ester Pagano, Alberta Bergamo, Sara Carpi

и другие.

Опубликована: Май 1, 2021

A wide range of experimental tumour models, each with distinct advantages and disadvantages, is nowadays available.Due to the inherent differences in their complexity functionality, choice model usually dependent on application.Thus, advance specific knowledge, one has choose use appropriate which largely hypotheses test, that objectives.Whatever chosen, cancer such none them will be able fully represent it.In vitro models have provided important tools for research still serve as low-cost screening platforms drugs.The improved understanding "organ system" pushed increased accuracy physiological relevance parallel complexity, diversifying output parameters they progressed view recapitulate most critical aspects dimensionality cell cultures (2D versus 3D), mechanical stimuli, multicellular interactions, immune interactions soluble signalling.Animal vivo counterpart lines are commonly used studies during preclinical investigation therapy determine efficacy safety novel drugs.They superior terms offering imitation parental tumours a heterogeneous microenvironment part an interacting complex biochemical system.In present review we describe limits major Oncological Pharmacology. Impact statementIntegration vitro, silico tumor vital obtain relevant data predictive toxicity

Язык: Английский

Процитировано

5

Objektive Zellkultur-Qualitätskontrolle durch infrarotspektroskopische Untersuchungen DOI

Miriam Unger,

Helmut Köster,

Alice Popescu

и другие.

BIOspektrum, Год журнала: 2014, Номер 20(7), С. 770 - 772

Опубликована: Ноя. 1, 2014

Язык: Английский

Процитировано

0

Detection of Exogenous and Endogenous (Cell Culture Based Analysis) Production of Cellulase in Grass Carp (Ctenopharyngodon idella) DOI Creative Commons

Pushpita Mishra,

Sriprakash Mohanty,

Mrinal Samanta

и другие.

Deleted Journal, Год журнала: 2016, Номер 2(1), С. 23 - 31

Опубликована: Янв. 1, 2016

Cellulase activity was found in forty eight bacteria isoalted from the grass carp intestine and intestinal content samples. Twenty six were positive towards congo red reaction. β-glucosidase exoglucanase measured bacterial cultures but endoglucanase could not be detected. This paper reports first attempt of using hepatopancreatic cell culture for establishment endogenous production cellulase Ctenopharyngodon idella (grass carp). EMEM media used culture. Initial experiment primary by trypsinization showed no enzyme (β-glucosidase) detected explant An increase (80 % 66 samples) seen when CMC as inducer

Язык: Английский

Процитировано

0

Dispositivos poliméricos para el confinamiento neuronal y registro electrofisiológico "in vitro". Métodos de fabricación y prueba de concepto DOI Open Access
Ricardo Morales

Опубликована: Окт. 9, 2013

La presente tesis trata del diseño y fabricación de un nuevo dispositivo para registrar la actividad eléctrica neuronal espontánea estimulada farmacológicamente, en redes neuronales in vitro. forma operar esta herramienta es simple, su producción bajo coste además, ofrece una interfaz neurona-electrodo estable a largo plazo más allá lo posible con tecnologías electrofisiológicas basadas micropipeta. El pieza polimérica (polidimetilsiloxano) dos compartimientos (zona sembrado los cultivos neuronales) comunicados entre sí través microcanal dimensiones comparables las axones dendritas. registrable tras el crecimiento espontáneo neuritas dentro (10 14 días sembradas neuronas). Un par electrodos, ubicados extremo microcanal, aprovechan propiedades conductoras medio extracelular potenciales eléctricos. tecnología microfabricar combinación técnica fotolitografía (para crear másteres) elastomer casting fabricar piezas poliméricas). procesamiento datos señales algoritmo matemático diseñado analizar información registrada por dispositivo; este ejecutado software “Matlab”. asigna contabiliza como acción que sobrepasen umbral predeterminado (170 μV) registros presenta resultados gráficas barras número unidad tiempo (minuto) The thesis describes the design and manufacturing of new tool to support electrophysiological investigation both individual electrical activity networks operation mode is easy learn device can be produced at low cost. Neuron-electrode interface stability better than electrophysiology micropipette interface. two compartments polymeric (polydimetilsiloxane) structure (neurons are placed) interconnected by microchannel. microchannel aperture comparable axon dendrites diameter. recorded after spontaneous neurites grew inside days neurons culturated). Each compartment has an electrode conductivity propriety extracellular medium supports record potentials end-to-end microfabrication process combination photolithography technique (to create masters) manufacture parts). Spike frequency analysis mathematical algorithm designed. runs Matlab software. counts higher 170 μV as action potential. Results presented graphic bars spike amount per time (minute).

Процитировано

0

Estudo da fotocitotoxicidade dos corantes ciânicos com dois cromóforos em culturas de células neoplásicas DOI Creative Commons

Luciana Sayuri Murakami

Опубликована: Окт. 29, 2009

Os corantes ciânicos com dois cromóforos possuem características espectrais e energéticas vantajosas para aplicação em Terapia Fotodinâmica (TFD) do câncer.Entretanto, sua fotoatividade contra neoplasias não foi ainda estudada nem in vivo vitro.Nesta tese apresentamos os resultados dos estudos vitro mecanismos da fotocitotoxicidade (BCD α ) ângulos entre = 180 0 , 150 90 células neoplásicas, a finalidade de avaliar potencialidade BCD como fotossensibilizadores (FS) TFD.Os foram realizados comparação o fotossensibilizador Photogem®,que já está sendo aplicado TFD.Foram estudados efeito fototóxico, distribuição intracelular contribuição apoptose necrose na morte celular induzida por ele.Além disso, realizada busca formulação farmacêutica adequada tópica .Nos utilizadas as neoplásicas melanoma murino B16F10, humano C8161, adenocarcinoma colo retal HT29, leucemia T (

Процитировано

0