2D and 3D cell cultures – a comparison of different types of cancer cell cultures
Archives of Medical Science,
Год журнала:
2016,
Номер
unknown
Опубликована: Янв. 1, 2016
Cell
culture
is
a
widely
used
in
vitro
tool
for
improving
our
understanding
of
cell
biology,
tissue
morphology,
and
mechanisms
diseases,
drug
action,
protein
production
the
development
engineering.
Most
research
regarding
cancer
biology
based
on
experiments
using
two-dimensional
(2D)
cultures
vitro.
However,
2D
have
many
limitations,
such
as
disturbance
interactions
between
cellular
extracellular
environments,
changes
polarity,
method
division.
These
disadvantages
led
to
creation
models
which
are
more
closely
able
mimic
conditions
vivo.
One
three-dimensional
(3D).
Optimisation
may
allow
better
facilitate
study
biomarkers
targeting
therapies.
In
this
review,
we
compare
3D
well
different
versions
cultures.
Язык: Английский
New Insights into Cell Culture Technology
Sivakumar Joghi Thatha Gowder
InTech eBooks,
Год журнала:
2017,
Номер
unknown
Опубликована: Май 10, 2017
The
book
"New
Insights
into
Cell
Culture
Technology"
focuses
on
many
advanced
methods
and
techniques
concerned
with
cell
culture.
contributing
authors
have
discussed
various
developments
in
culture
methods,
the
application
of
insect
cells
for
efficient
production
heterologous
proteins,
expansion
human
mesenchymal
stromal
different
clinical
applications,
remote
sensing
experiments
concepts
development
bioprocess,
continuous
retroviral
pseudotype
vectors,
oncolytic
measles
virus
vectors
cancer
therapy.
This
is
an
original
contribution
experts
from
parts
globe,
in-depth
information
will
be
a
significant
resource
students,
scientists,
physicians
who
are
directly
dealing
cells.
["Culture"
essential
life
also
cell.
-
Sivakumar
Gowder]
Язык: Английский
Current approaches and techniques of 3D cell culture systems: a review
Archives of Ecotoxicology,
Год журнала:
2024,
Номер
6(1), С. 22 - 27
Опубликована: Июнь 29, 2024
Presently,
the
majority
of
cells
are
cultivated
by
two-dimensional
(2D)
methods;
however,
latest
and
enhanced
procedures
employing
three-dimensional
(3D)
cell
culturing
techniques
provide
strong
indications
that
significantly
more
sophisticated
studies
can
be
carried
out,
providing
invaluable
insights.
Recent
years
have
seen
a
rapid
development
3D
cultures
since
grown
in
static
environment
on
flat
substrate
far
from
reaching
an
vivo
condition.
Currently,
scientists
gradually
realising
vitro
shape,
structure,
physiological
activities
may
achieved.
As
resolution,
matrix-like
framework
for
attachment,
proliferation,
differentiation,
communication
both
dynamic
culture
conditions
is
what
carriers
come
to
offer.
Different
mechanical
stimulations
closely
resemble
genuine
microenvironment
could
main
function
systems.
Current
developments
been
presented
this
review,
along
with
discussion
their
benefits
drawbacks
when
compared
conventional
2D
growth
under
settings.
Язык: Английский
Porcine parvovirus as a contaminant in cell cultures and laboratory supplies
Biologicals,
Год журнала:
2016,
Номер
44(2), С. 53 - 59
Опубликована: Янв. 19, 2016
Язык: Английский
Preclinical models in oncological pharmacology: limits and advantages
Опубликована: Май 1, 2021
A
wide
range
of
experimental
tumour
models,
each
with
distinct
advantages
and
disadvantages,
is
nowadays
available.Due
to
the
inherent
differences
in
their
complexity
functionality,
choice
model
usually
dependent
on
application.Thus,
advance
specific
knowledge,
one
has
choose
use
appropriate
which
largely
hypotheses
test,
that
objectives.Whatever
chosen,
cancer
such
none
them
will
be
able
fully
represent
it.In
vitro
models
have
provided
important
tools
for
research
still
serve
as
low-cost
screening
platforms
drugs.The
improved
understanding
"organ
system"
pushed
increased
accuracy
physiological
relevance
parallel
complexity,
diversifying
output
parameters
they
progressed
view
recapitulate
most
critical
aspects
dimensionality
cell
cultures
(2D
versus
3D),
mechanical
stimuli,
multicellular
interactions,
immune
interactions
soluble
signalling.Animal
vivo
counterpart
lines
are
commonly
used
studies
during
preclinical
investigation
therapy
determine
efficacy
safety
novel
drugs.They
superior
terms
offering
imitation
parental
tumours
a
heterogeneous
microenvironment
part
an
interacting
complex
biochemical
system.In
present
review
we
describe
limits
major
Oncological
Pharmacology.
Impact
statementIntegration
vitro,
silico
tumor
vital
obtain
relevant
data
predictive
toxicity
Язык: Английский
Objektive Zellkultur-Qualitätskontrolle durch infrarotspektroskopische Untersuchungen
Miriam Unger,
Helmut Köster,
Alice Popescu
и другие.
BIOspektrum,
Год журнала:
2014,
Номер
20(7), С. 770 - 772
Опубликована: Ноя. 1, 2014
Язык: Английский
Detection of Exogenous and Endogenous (Cell Culture Based Analysis) Production of Cellulase in Grass Carp (Ctenopharyngodon idella)
Deleted Journal,
Год журнала:
2016,
Номер
2(1), С. 23 - 31
Опубликована: Янв. 1, 2016
Cellulase
activity
was
found
in
forty
eight
bacteria
isoalted
from
the
grass
carp
intestine
and
intestinal
content
samples.
Twenty
six
were
positive
towards
congo
red
reaction.
β-glucosidase
exoglucanase
measured
bacterial
cultures
but
endoglucanase
could
not
be
detected.
This
paper
reports
first
attempt
of
using
hepatopancreatic
cell
culture
for
establishment
endogenous
production
cellulase
Ctenopharyngodon
idella
(grass
carp).
EMEM
media
used
culture.
Initial
experiment
primary
by
trypsinization
showed
no
enzyme
(β-glucosidase)
detected
explant
An
increase
(80
%
66
samples)
seen
when
CMC
as
inducer
Язык: Английский
Dispositivos poliméricos para el confinamiento neuronal y registro electrofisiológico "in vitro". Métodos de fabricación y prueba de concepto
Опубликована: Окт. 9, 2013
La
presente
tesis
trata
del
diseño
y
fabricación
de
un
nuevo
dispositivo
para
registrar
la
actividad
eléctrica
neuronal
espontánea
estimulada
farmacológicamente,
en
redes
neuronales
in
vitro.
forma
operar
esta
herramienta
es
simple,
su
producción
bajo
coste
además,
ofrece
una
interfaz
neurona-electrodo
estable
a
largo
plazo
más
allá
lo
posible
con
tecnologías
electrofisiológicas
basadas
micropipeta.
El
pieza
polimérica
(polidimetilsiloxano)
dos
compartimientos
(zona
sembrado
los
cultivos
neuronales)
comunicados
entre
sí
través
microcanal
dimensiones
comparables
las
axones
dendritas.
registrable
tras
el
crecimiento
espontáneo
neuritas
dentro
(10
14
días
sembradas
neuronas).
Un
par
electrodos,
ubicados
extremo
microcanal,
aprovechan
propiedades
conductoras
medio
extracelular
potenciales
eléctricos.
tecnología
microfabricar
combinación
técnica
fotolitografía
(para
crear
másteres)
elastomer
casting
fabricar
piezas
poliméricas).
procesamiento
datos
señales
algoritmo
matemático
diseñado
analizar
información
registrada
por
dispositivo;
este
ejecutado
software
“Matlab”.
asigna
contabiliza
como
acción
que
sobrepasen
umbral
predeterminado
(170
μV)
registros
presenta
resultados
gráficas
barras
número
unidad
tiempo
(minuto)
The
thesis
describes
the
design
and
manufacturing
of
new
tool
to
support
electrophysiological
investigation
both
individual
electrical
activity
networks
operation
mode
is
easy
learn
device
can
be
produced
at
low
cost.
Neuron-electrode
interface
stability
better
than
electrophysiology
micropipette
interface.
two
compartments
polymeric
(polydimetilsiloxane)
structure
(neurons
are
placed)
interconnected
by
microchannel.
microchannel
aperture
comparable
axon
dendrites
diameter.
recorded
after
spontaneous
neurites
grew
inside
days
neurons
culturated).
Each
compartment
has
an
electrode
conductivity
propriety
extracellular
medium
supports
record
potentials
end-to-end
microfabrication
process
combination
photolithography
technique
(to
create
masters)
manufacture
parts).
Spike
frequency
analysis
mathematical
algorithm
designed.
runs
Matlab
software.
counts
higher
170
μV
as
action
potential.
Results
presented
graphic
bars
spike
amount
per
time
(minute).
Estudo da fotocitotoxicidade dos corantes ciânicos com dois cromóforos em culturas de células neoplásicas
Опубликована: Окт. 29, 2009
Os
corantes
ciânicos
com
dois
cromóforos
possuem
características
espectrais
e
energéticas
vantajosas
para
aplicação
em
Terapia
Fotodinâmica
(TFD)
do
câncer.Entretanto,
sua
fotoatividade
contra
neoplasias
não
foi
ainda
estudada
nem
in
vivo
vitro.Nesta
tese
apresentamos
os
resultados
dos
estudos
vitro
mecanismos
da
fotocitotoxicidade
(BCD
α
)
ângulos
entre
=
180
0
,
150
90
células
neoplásicas,
a
finalidade
de
avaliar
potencialidade
BCD
como
fotossensibilizadores
(FS)
TFD.Os
foram
realizados
comparação
o
fotossensibilizador
Photogem®,que
já
está
sendo
aplicado
TFD.Foram
estudados
efeito
fototóxico,
distribuição
intracelular
contribuição
apoptose
necrose
na
morte
celular
induzida
por
ele.Além
disso,
realizada
busca
formulação
farmacêutica
adequada
tópica
.Nos
utilizadas
as
neoplásicas
melanoma
murino
B16F10,
humano
C8161,
adenocarcinoma
colo
retal
HT29,
leucemia
T
(