Evaluating the Efficacy of Serum-Free Media Supplemented with Protein Isolates for Bovine Satellite Cell Proliferation: A Sustainable Approach for Cultivated Meat Production DOI Creative Commons

Arian Amirvaresi,

Reza Ovissipour

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 26, 2024

Abstract This study investigates the short- and long-term efficacy of serum-free media supplemented with protein isolates from algae, alfalfa, silkworm pupae, grasshoppers for proliferation bovine satellite cells (BSCs). Fresh spent were analyzed to monitor metabolites, while cell was assessed using CyQUANT assay. The results short-term growth indicated that a lower concentration alfalfa isolate (0.05 mg/mL) significantly enhanced proliferation, achieving 1.47-fold increase compared basal media, thereby demonstrating its potential as viable alternative fetal serum (FBS). capability developed passaging examined different coating strategies, vitronectin outperforming others; however, it unable support FBS-containing media. Given critical role glutamine, this also evaluates impact L-glutamine supplementation at concentrations on metabolism in alfalfa-based However, glutamine showed trends toward increased growth, enhancements not statistically significant. Based results, highlights promising component BSC underscores need continued research into sources formulations sustainable ethical production cultivated meat.

Язык: Английский

Sustainable Alternatives to Fetal Bovine Serum: Evaluating the Role of Plant and Insect Protein Isolates in Serum-Free Media for Bovine Satellite Cell Proliferation in Cultivated Meat Production DOI

Arian Amirvaresi,

Reyhaneh Sarkarat,

C. R. Jones

и другие.

ACS Food Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

Язык: Английский

Процитировано

0

Evaluating the Efficacy of Serum-Free Media Supplemented with Protein Isolates for Bovine Satellite Cell Proliferation: A Sustainable Approach for Cultivated Meat Production DOI Creative Commons

Arian Amirvaresi,

Reza Ovissipour

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 26, 2024

Abstract This study investigates the short- and long-term efficacy of serum-free media supplemented with protein isolates from algae, alfalfa, silkworm pupae, grasshoppers for proliferation bovine satellite cells (BSCs). Fresh spent were analyzed to monitor metabolites, while cell was assessed using CyQUANT assay. The results short-term growth indicated that a lower concentration alfalfa isolate (0.05 mg/mL) significantly enhanced proliferation, achieving 1.47-fold increase compared basal media, thereby demonstrating its potential as viable alternative fetal serum (FBS). capability developed passaging examined different coating strategies, vitronectin outperforming others; however, it unable support FBS-containing media. Given critical role glutamine, this also evaluates impact L-glutamine supplementation at concentrations on metabolism in alfalfa-based However, glutamine showed trends toward increased growth, enhancements not statistically significant. Based results, highlights promising component BSC underscores need continued research into sources formulations sustainable ethical production cultivated meat.

Язык: Английский

Процитировано

2