bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 26, 2024
Abstract This study investigates the short- and long-term efficacy of serum-free media supplemented with protein isolates from algae, alfalfa, silkworm pupae, grasshoppers for proliferation bovine satellite cells (BSCs). Fresh spent were analyzed to monitor metabolites, while cell was assessed using CyQUANT assay. The results short-term growth indicated that a lower concentration alfalfa isolate (0.05 mg/mL) significantly enhanced proliferation, achieving 1.47-fold increase compared basal media, thereby demonstrating its potential as viable alternative fetal serum (FBS). capability developed passaging examined different coating strategies, vitronectin outperforming others; however, it unable support FBS-containing media. Given critical role glutamine, this also evaluates impact L-glutamine supplementation at concentrations on metabolism in alfalfa-based However, glutamine showed trends toward increased growth, enhancements not statistically significant. Based results, highlights promising component BSC underscores need continued research into sources formulations sustainable ethical production cultivated meat.
Язык: Английский