Taxonomic Identification, Complete Genome Sequencing, and In Silico Genome Mining of the Actinobacterium Lentzea sp. JNUCC 0626 Isolated from Jeju Gotjawal
Deleted Journal,
Год журнала:
2025,
Номер
70(1), С. 8 - 8
Опубликована: Фев. 7, 2025
In
our
previous
study,
Lentzea
sp.
JNUCC
0626
was
isolated
from
Hwasun
Gotjawal
on
Jeju
Island,
and
its
melanogenic
effects
were
confirmed
in
B16F10
melanoma
cells
through
the
identification
of
1-acetyl-β-carboline.
this
we
conducted
a
comprehensive
taxonomic
characterization
0626,
including
enzymatic
activities,
carbohydrate
metabolism,
growth
conditions,
cellular
composition.
Major
fatty
acids
identified
iso-C16:0,
iso-C15:0,
C15:0
anteiso,
with
polar
lipids
such
as
diphosphatidylglycerol,
phosphatidylethanolamine,
several
unidentified
lipids.
Ubiquinone
Q-9
determined
predominant
respiratory
quinone.
Enzymatic
activity
analysis
(API
ZYM)
showed
alkaline
phosphatase,
esterase
(C4),
lipase
(C8),
leucine
arylamidase
while
metabolism
50CHB)
indicated
acid
production
esculin
alone.
Complete
genome
sequencing
revealed
10,602,950
bp
linear
chromosome
177,940
plasmid.
This
plasmid
encodes
essential
plasmid-related
genes,
Type
IV
secretion
system
ParA
proteins
critical
for
transfer
stability.
These
findings
suggest
that
could
be
utilized
developing
host–vector
systems
to
facilitate
combinatorial
biosynthesis
novel
bioactive
compounds.
Comparative
genomic
pudingi
CGMCC
4.7319
closest
relative,
but
significant
genetic
divergence
(dDDH
46.7%,
ANI
88.02%)
strongly
supports
classification
species.
AntiSMASH
34
biosynthetic
gene
clusters
(BGCs),
highlighting
strain’s
capacity
produce
diverse
Finally,
extract
exhibited
concentration-dependent
NO
inhibition
LPS-stimulated
RAW
264.7
cells,
demonstrating
anti-inflammatory
activity.
suggests
secondary
metabolites
inferred
may
contribute
these
observed
bioactivities.
Язык: Английский
Unique microbial communities in ancient volcanic ash layers within deep marine sediments are structured by the composition of iron phases
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Март 12, 2025
Much
of
the
marine
sedimentary
environment
is
affected
by
deposition
tephra,
explosive
products
volcanic
eruptions.
These
tephra
layers’
geochemical
and
physical
properties
often
differ
substantially
from
those
surrounding
sediment,
forming
an
extreme
carbon-lean
within
anoxic
deep
biosphere.
Despite
this,
evidence
suggests
layers
harbor
diverse
abundant
microbial
communities.
While
little
known
about
composition
these
communities
even
less
their
life
modes,
there
indicating
that
iron
(Fe)
plays
a
vital
role
for
microorganisms.
Here,
we
aim
to
link
differences
in
content
sediments
with
changes
We
combined
next-generation
sequencing
16S
rRNA
genes
analyses
Fe
phases
preserved
ancient
recovered
Norwegian
Margin
during
Expedition
396
International
Ocean
Discovery
Program
(IODP).
In
samples,
basaltic
contained
nearly
double
total
as
sediments,
majority
hosted
“reducible”
Fe(III)
oxides,
whilst
primarily
“easily
reducible”
oxides.
Basaltic
harbored
distinct
differed
sediment
predicted
metabolic
properties.
predictions
suggest
higher
potential
assimilatory
use
more
complex
sources
microbes
are
able
exploit
found
high
quantities
layers.
Our
findings
confirm
few
previous
studies
have
suggested
occur
Deciphering
indigenous
microorganisms
hints
at
how
might
flourish
this
environment.
This
has
implications
understanding
ubiquitous
component
Язык: Английский
Borrelidin M: a new borrelidin derivative obtained from Streptomyces rochei VL-16 exhibited potent antibacterial and antiproliferative properties
Vinoth Vengadesan,
Jagan Muniyandi,
Neha Yadav
и другие.
International Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 15, 2025
Язык: Английский
Decylprodigiosin: a new member of the prodigiosin family isolated from a seaweed-associated Streptomyces
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Март 21, 2024
Bioprospecting
actinobacterial
secondary
metabolism
from
untapped
marine
sources
may
lead
to
the
discovery
of
biotechnologically-relevant
compounds.
While
studying
diversity
and
bioactive
potential
Actinomycetota
associated
with
Codium
tomentosum,
a
green
seaweed
collected
in
northern
Portuguese
cost,
strain
CT-F61,
identified
as
Streptomyces
violaceoruber
,
was
isolated.
Its
extracts
displayed
strong
anticancer
activity
on
breast
carcinoma
T-47D
colorectal
HCT116
cells,
being
effective
well
against
panel
human
fish
pathogenic
bacteria.
Following
bioactivity-guided
isolation
pipeline,
new
analogue
red-pigmented
family
antibiotics
prodigiosins,
decylprodigiosin
(1),
chemically
characterized.
Despite
this
natural
products
well-known
for
long
time,
we
report
first
evidence
prodigiosins
produced
by
seaweed-associated
actinomycete.
Язык: Английский
Cellulamides: A New Family of Marine-Sourced Linear Peptides from the Underexplored Cellulosimicrobium Genus
Marine Drugs,
Год журнала:
2024,
Номер
22(6), С. 268 - 268
Опубликована: Июнь 11, 2024
Bioprospecting
the
secondary
metabolism
of
underexplored
Actinomycetota
taxa
is
a
prolific
route
to
uncover
novel
chemistry.
In
this
work,
we
report
isolation,
structure
elucidation,
and
bioactivity
screening
cellulamides
A
B
(1
2),
two
linear
peptides
obtained
from
culture
macroalga-associated
Cellulosimicrobium
funkei
CT-R177.
The
host
microorganism,
Chlorophyta
Codium
tomentosum,
was
collected
in
northern
Portuguese
coast
and,
scope
bioprospecting
study
focused
on
its
associated
actinobacterial
community,
strain
CT-R177
isolated,
taxonomically
identified,
screened
for
production
antimicrobial
anticancer
compounds.
Dereplication
crude
extract
using
LC-HRMS(/MS)
analysis
unveiled
putative
natural
product,
cellulamide
(1),
that
isolated
following
mass
spectrometry-guided
fractionation.
An
additional
analog,
(2)
during
chromatographic
process
chemically
characterized.
chemical
structures
peptides,
including
their
absolute
configurations,
were
elucidated
combination
HRMS,
1D/2D
NMR
spectroscopy,
Marfey’s
analysis.
Cellulamide
(1)
subjected
set
screenings,
but
no
significant
biological
activity
observed.
represent
first
family
products
reported
genus
Cellulosimicrobium,
showcasing
not
only
potential
less-explored
also
host-associated
marine
strains
chemistry
discovery.
Язык: Английский
Salt Tolerant Microbes (Active Metabolites) Mediated Nanoparticle: Drug Delivery and Future Prospects
Journal of Pure and Applied Microbiology,
Год журнала:
2024,
Номер
18(2), С. 853 - 866
Опубликована: Июнь 1, 2024
Salt
Tolerant
Microbes
are
a
group
of
microorganisms
that
grow,
develop,
and
survive
in
extremely
high
salt
concentrations.
Based
on
their
tolerance
level
they
generally
grow
up
optimally
at
pH
values
beyond
9.0,
but
the
growth
is
inhibited
value
most
closely
associated
with
neutral
6.5.
They
have
minimal
dietary
needs
good
quantity
enough
to
osmotic
pressure.
can
produce
biological
metabolites
certain
actions
such
as
antibacterial,
antifungal,
antioxidant,
anticancer.
We
discussed
this
article
various
pharmaceutical
formulations
salt-tolerant
microbes,
every
formulation
shows
specific
pharmacological
like
anti-cancer
activity,
anti-oxidant
anti-microbial
also
discusses
methods
for
biosynthesis
microbes’
nanoparticles.
Язык: Английский