Protective effect of ginseng seed oil nanoemulsion against human coronavirus OC43 through modulation of ABCG transporters

Journal of Functional Foods, Год журнала: 2024, Номер 117, С. 106240 - 106240

Опубликована: Май 20, 2024

Recent studies have highlighted the potential of unsaturated fatty acids in natural oils to influence virus replication. This study extracted ginseng seed oil (GSO) and evaluated its physiochemical parameters, lipid class, oxidation stability. GSO was then synthesized into an oil/water nanoemulsion (NE) through optimized formulation, time, temperature, storage conditions. GSO-NE showed no cytotoxicity up 5 µg/mL but effectively inhibited HCoV-OC43 replication suppressed proinflammatory biomarkers infected cells. RNA sequencing revealed significant alterations gene expression profile by treatment, particularly implicating ABC transporters signaling pathway. Notably, downregulated ABCG subfamily membrane proteins, crucial for regulation virus-infected The downregulation these genes (ABCG1, ABCG2, ABCG4, ABCG5/8) reversed GSO-NE, as demonstrated qRT-PCR. Furthermore, ABCG1 ABCG5/8 confirmed using immunoblotting techniques. These findings suggest a promising avenue future research or therapeutic applications.

Язык: Английский

---

The Comparison of Classification Model with Partial Least Square Based Dimension Reduction

Lecture notes in electrical engineering, Год журнала: 2011, Номер unknown, С. 173 - 180

Опубликована: Янв. 1, 2011

Язык: Английский

---