L-asparaginase from the mangrove endophyte Penicillium citrinum MEF 455: a focus on cancer surveillance gene expression in tumor cell lines HL-60 and NCI-H 460 DOI

Neema Job,

K. S. Sruthy,

Divya Jose

и другие.

Toxicology Research, Год журнала: 2025, Номер 14(3)

Опубликована: Май 2, 2025

Abstract Marine endophytic fungi serve as a valuable source of bioactive molecules, with growing applications in enzyme production. This study investigates the therapeutic potential glutaminase- and urease-free Type II L-asparaginase derived from mangrove endophyte Penicillium citrinum MEF 455 against neoplastic cells. Extracellular production was done using Czapek Dox broth amended L-asparagine 66 kDa molecular mass asparaginase could be observed. The specific activity 41.6 U/mg 5.8-fold purification attained DEAE cellulose Sephacryl S-200 column. kinetic showed that Km, Vmax, Kcat were 1.370 mM, 161.29 U/mL/min, 1240.69/min, respectively. Purified displayed optimal at 40 °C pH 8, substrate concentration 2.5 mM L-asparagine. Metallic ions like Na+, K+, Mg2+, Co2+, Li+, improved activity. strong anticancer considerable reduction growth HL-60, NCI-H 460 cells IC50 values 0.37 ± 0.225 U/mL 0.39 0.176 U/mL, Major cancer-controlling genes i.e. p53, caspase-3, caspase-9, NF-kB, Bax, Rb1 up-regulated. In contrast, anti-apoptotic Cox-2 Bcl-2 down-regulated on treatment Human cancer cell lines HL-60 460. experimental demonstrates produced an fungal source, P. 455, free glutaminase urease activity, thereby minimizing associated immunogenic complications. general, understanding physicochemical properties functionality highlights its promising antitumor candidate for development clinical applications.

Язык: Английский

L-asparaginase from the mangrove endophyte Penicillium citrinum MEF 455: a focus on cancer surveillance gene expression in tumor cell lines HL-60 and NCI-H 460 DOI

Neema Job,

K. S. Sruthy,

Divya Jose

и другие.

Toxicology Research, Год журнала: 2025, Номер 14(3)

Опубликована: Май 2, 2025

Abstract Marine endophytic fungi serve as a valuable source of bioactive molecules, with growing applications in enzyme production. This study investigates the therapeutic potential glutaminase- and urease-free Type II L-asparaginase derived from mangrove endophyte Penicillium citrinum MEF 455 against neoplastic cells. Extracellular production was done using Czapek Dox broth amended L-asparagine 66 kDa molecular mass asparaginase could be observed. The specific activity 41.6 U/mg 5.8-fold purification attained DEAE cellulose Sephacryl S-200 column. kinetic showed that Km, Vmax, Kcat were 1.370 mM, 161.29 U/mL/min, 1240.69/min, respectively. Purified displayed optimal at 40 °C pH 8, substrate concentration 2.5 mM L-asparagine. Metallic ions like Na+, K+, Mg2+, Co2+, Li+, improved activity. strong anticancer considerable reduction growth HL-60, NCI-H 460 cells IC50 values 0.37 ± 0.225 U/mL 0.39 0.176 U/mL, Major cancer-controlling genes i.e. p53, caspase-3, caspase-9, NF-kB, Bax, Rb1 up-regulated. In contrast, anti-apoptotic Cox-2 Bcl-2 down-regulated on treatment Human cancer cell lines HL-60 460. experimental demonstrates produced an fungal source, P. 455, free glutaminase urease activity, thereby minimizing associated immunogenic complications. general, understanding physicochemical properties functionality highlights its promising antitumor candidate for development clinical applications.

Язык: Английский

Процитировано

0