ACS Applied Materials & Interfaces,
Год журнала:
2023,
Номер
15(30), С. 36061 - 36075
Опубликована: Июль 18, 2023
Ulcerative
colitis
(UC)
is
a
chronic
inflammatory
bowel
disease
of
unknown
etiology
affecting
the
colon
and
rectum.
Current
therapeutics
are
focused
on
suppressing
inflammation
but
ineffective.
Combining
anti-inflammatory
therapeutic
approaches
with
pro-resolution
might
be
superior
strategy
for
UC
treatment.
Andrographolide
(AG),
an
active
compound
from
plant
Andrographis
paniculata,
presented
effects
in
various
diseases.
Gaseous
mediators,
such
as
carbon
monoxide
(CO),
have
role
resolution.
Herein,
we
developed
dextran-functionalized
PLGA
nanocarrier
efficient
delivery
AG
donor
(CORM-2)
synergistically
anti-inflammatory/pro-resolving
treatment
(AG/CORM-2@NP-Dex)
based
good
biocompatibility,
slow
drug
release,
targeting,
biodegradability.
The
resulting
had
nano-scaled
diameter
∼200
nm
spherical
shape.
After
being
coated
dextran
(Dex),
AG/CORM-2@NP-Dex
could
efficiently
internalized
by
Colon-26
Raw
264.7
cells
vitro
preferentially
localized
to
inflamed
chitosan/alginate
hydrogel
protection
gavage.
performed
eliminating
over-production
pro-inflammatory
mediator,
nitric
oxide
(NO),
down-regulating
expression
cytokines
(TNF-α,
IL-1β
IL-6),
while
it
showed
pro-resolving
function
accelerating
M1
M2
macrophage
conversion
up-regulating
resolution-related
genes
(IL-10,
TGF-β,
HO-1).
In
model,
oral
administration
also
effects,
therefore
relieving
effectively.
Without
appreciable
systemic
toxicity,
this
bifunctional
represents
novel
approach
expected
achieve
long-term
remission.
EBioMedicine,
Год журнала:
2019,
Номер
44, С. 322 - 333
Опубликована: Май 28, 2019
Gastric
cancer
(GC)
is
the
third
leading
cause
of
death.
Early
detection
a
key
factor
to
reduce
its
mortality.We
retrospectively
collected
pre-
and
postoperative
serum
samples
as
well
tumour
tissues
adjacent
normal
from
100
GC
patients.
Serum
non-cancerous
patients
were
served
controls
(n
=
50).
A
high-throughput
protein
technology,
multiplex
proximity
extension
assays
(PEA),
was
applied
measure
levels
over
300
proteins.
Alteration
each
analysed
by
univariate
analysis.
Elastic-net
logistic
regression
performed
select
proteins
into
diagnostic
model.We
identified
19
(CEACAM5,
CA9,
MSLN,
CCL20,
SCF,
TGF-alpha,
MMP-1,
MMP-10,
IGF-1,
CDCP1,
PPIA,
DDAH-1,
HMOX-1,
FLI1,
IL-7,
ZBTB-17,
APBB1IP,
KAZALD-1,
ADAMTS-15)
that
together
distinguish
cases
with
sensitivity
93%,
specificity
100%,
area
under
receiver
operating
characteristic
curve
(AUC)
0·99
(95%
CI:
0·98-1).
Moreover,
19-serum
signature
provided
an
increased
capacity
in
at
TNM
I-II
stage
(sensitivity
89%,
AUC
0·99)
high
microsatellite
instability
(MSI)
(91%,
98%,
compared
individual
These
promising
results
will
inspire
large-scale
independent
cohort
study
be
pursued
for
validating
proposed
signature.Based
on
targeted
proteomics
elastic-net
regression,
we
which
could
contribute
clinical
diagnosis,
especially
early
those
MSI.
FUND:
This
supported
European
H2020-Marie
Skłodowska-Curie
Innovative
Training
Networks
grant
(316,929,
GastricGlycoExplorer).
Funder
had
no
influence
trial
design,
data
evaluation,
interpretation.
Biomedicine & Pharmacotherapy,
Год журнала:
2018,
Номер
109, С. 112 - 119
Опубликована: Ноя. 2, 2018
Farrerol,
an
important
bioactive
constituent
of
rhododendron,
exhibits
broad
activities
such
as
anti-oxidative
and
anti-inflammatory
effects.
Recent
studies
showed
that
farrerol
possesses
neuroprotective
activity,
however,
the
mechanism
has
not
been
reported.
The
aim
present
study
was
to
investigate
protective
effect
on
β-amyloid
(Aβ)-induced
mouse
microglial
BV-2
cells
underlying
mechanism.
were
pretreated
with
for
1
h
then
subjected
Aβ.
MTT
assay
performed
measure
mitochondrial
metabolic
activity
in
cells.
production
reactive
oxygen
species
(ROS)
malondialdehyde
(MDA)
superoxide
dismutase
(SOD)
detected
reflect
oxidative
stress
status.
secretion
mRNA
levels
interleukin
(IL)-1β
(IL-1β),
IL-6
tumor
necrosis
factor-α
(TNF-α)
measured
by
ELISA
qRT-PCR.
expressions
NF-E2-related
factor
(Nrf2),
heme
oxygenase-1
(HO-1),
NAD
(P)
H:
quinone
oxidoreductase
(NQO1),
Kelch-like
ECH-associated
protein
(Keap1)
western
blot.
Our
results
improved
Aβ-induced
Aβ
induced
ROS
MDA,
inhibited
SOD
expression
SOD1
SOD2
mRNA,
while
effects
attenuated
farrerol.
Farrerol
also
induction
IL-6,
IL-1β,
TNF-α.
In
addition,
enhanced
activation
Nrf2/Keap1
pathway
Knockdown
Nrf2
small
interfere
RNA
(siRNA)
targeting
(si-Nrf2)
abolished
conclusion,
inflammation
through
enhancing
pathway.
findings
indicated
could
be
considered
a
therapeutic
approach
treatment
Alzheimer's
disease
(AD).
Heme
oxygenase-1
(HO-1),
a
rate-limiting
enzyme
involved
in
the
degradation
of
heme,
is
induced
response
to
wide
range
stress
conditions.
HO-1
exerts
antiviral
activity
against
broad
viruses,
including
Hepatitis
C
virus,
human
immunodeficiency
and
dengue
virus
by
inhibiting
viral
growth.
It
has
been
reported
that
displays
Zika
(ZIKV)
but
mechanisms
inhibition
remain
largely
unknown.
Using
ZIKV
RNA
replicon
with
Green
Fluorescent
Protein
(GFP)
as
reporter
protein,
we
were
able
show
expression
resulted
replication.
Conversely,
observed
decrease
cells
replicating
replicon.
The
study
infected
showed
level
was
significantly
lower
once
replication
established,
thereby
limiting
effect
HO-1.
Our
work
highlights
capacity
thwart
anti-replicative
cells.
Therefore,
modulation
novel
therapeutic
strategy
infection
may
display
limited
effect.
Chemical Reviews,
Год журнала:
2020,
Номер
120(24), С. 13273 - 13311
Опубликована: Окт. 22, 2020
Nature
is
full
of
examples
symbiotic
relationships.
The
critical
relation
between
host
and
mutualistic
bacteria
attracting
increasing
attention
to
the
degree
that
gut
microbiome
proposed
by
some
as
a
new
organ
system.
exerts
its
systemic
effect
through
diverse
range
metabolites,
which
include
gaseous
molecules
such
H2,
CO2,
NH3,
CH4,
NO,
H2S,
CO.
In
turn,
human
can
influence
these
well
in
reciprocal
manner.
Among
molecules,
CO
occupy
special
place
because
their
widely
known
physiological
functions
overlap
similarity
both
targets
functions.
roles
NO
H2S
play
have
been
extensively
examined
others.
Herein,
host-gut
communication
are
discussion
(1)
production
function
CO,
(2)
available
donors
research
tools,
(3)
from
diet
bacterial
sources,
(4)
on
including
sensing,
(5)
There
large
amount
literature
suggesting
"messenger"
role
communication.
However,
much
more
work
needed
begin
achieving
systematic
understanding
this
issue.
Antioxidants,
Год журнала:
2020,
Номер
9(11), С. 1153 - 1153
Опубликована: Ноя. 19, 2020
Heme
oxygenase-1
(HO-1)
is
an
inducible
stress
protein
that
catalyzes
the
oxidative
conversion
of
heme
to
carbon
monoxide
(CO),
iron,
and
biliverdin
(BV),
latter
which
converted
bilirubin
(BR)
by
reductase.
HO-1
has
been
implicated
as
a
cytoprotectant
in
various
models
acute
organ
injury
disease
(i.e.,
lung,
kidney,
heart,
liver).
Thus,
may
serve
general
therapeutic
target
inflammatory
diseases.
function
pleiotropic
modulator
signaling,
via
removal
heme,
generation
its
enzymatic
degradation-products.
Iron
release
from
HO
activity
exert
pro-inflammatory
effects
unless
sequestered,
whereas
BV/BR
have
well-established
antioxidant
properties.
CO,
derived
activity,
identified
endogenous
mediator
can
influence
mitochondrial
and/or
cellular
signal
transduction
programs
culminate
regulation
apoptosis,
proliferation,
inflammation.
Much
research
focused
on
application
low
concentration
whether
administered
gaseous
form
inhalation,
or
use
CO-releasing
molecules
(CORMs),
for
benefit
disease.
The
development
novel
CORMs
their
translational
potential
remains
active
area
investigation.
Evidence
accumulated
both
CO
diseases
associated
with
critical
care,
including
lung
injury/acute
respiratory
distress
syndrome
(ALI/ARDS),
mechanical
ventilation-induced
injury,
pneumonias,
sepsis.
benefits
extend
other
involving
aberrant
processes
such
transplant-associated
ischemia/reperfusion
chronic
graft
rejection,
metabolic
Current
planned
clinical
trials
explore
ARDS
