Abstract
Cuproptosis
is
a
novel
type
to
regulate
cell
death
with
copper-dependent
manner,
and
has
been
reported
involve
in
the
occurrence
development
of
various
malignant
tumors.
However,
association
between
cuproptosis
tumor
microenvironment
(TME)
clear
renal
carcinoma
(ccRCC)
remained
unclear.
To
address
this
question,
we
integrated
single
RNA
sequencing
(scRNA-seq)
datasets
ccRCC
across
different
stages,
systematically
examined
distinctive
expression
patterns
cuproptosis-related
genes
(CRGs)
within
TME
ccRCC,
explored
crucial
signatures
using
spatial
transcriptome
(ST-seq)
dataset.
The
activities
reduced
cancer
tissues
along
development,
recovered
after
therapy.
We
identified
HILPDA
+
ccRCC1
subtype,
characterized
hypoxia,
as
susceptible
cells
associated
better
prognosis.
main
co-expression
modules
subtype
highlighted
role
anion
transport,
response
oxygen
species
PD-L1-PD-1
pathway.
Furthermore,
immunosuppressive
might
interact
via
HAVCR2-LGALS9,
C3-C3AR1,
HLA-A-CD8B
HLA-C-CD8A
axises
shape
landscape.
In
summary,
anticipate
that
study
will
offer
valuable
insights
potential
strategies
for
therapy
ccRCC.
Graphical
Journal of Experimental & Clinical Cancer Research,
Год журнала:
2024,
Номер
43(1)
Опубликована: Март 5, 2024
Abstract
Background
Proteasome
inhibitors
(PIs)
are
one
of
the
most
important
classes
drugs
for
treatment
multiple
myeloma
(MM).
However,
almost
all
patients
with
MM
develop
PI
resistance,
resulting
in
therapeutic
failure.
Therefore,
mechanisms
underlying
resistance
require
further
investigation.
Methods
We
used
several
cell
lines
to
establish
PI-resistant
lines.
performed
RNA
microarray
and
EccDNA-seq
collected
human
primary
samples
explore
gene
profiles.
evaluated
effect
MUC20
on
cuproptosis
cells
using
Co-immunoprecipitation
(Co-IP),
Seahorse
bioenergetic
profiling
vivo
assay.
Results
This
study
revealed
that
downregulation
Mucin
20
(MUC20)
could
predict
sensitivity
outcomes
patients.
Besides,
attenuated
by
inducing
via
inhibition
cyclin-dependent
kinase
inhibitor
2
A
expression
(CDKN2A),
which
was
achieved
hindering
MET
proto-oncogene,
receptor
tyrosine
(MET)
activation.
Moreover,
suppressed
activation
repressing
insulin-like
growth
factor
receptor-1
(IGF-1R)
lactylation
cells.
is
first
perform
extrachromosomal
circular
DNA
(eccDNA)
sequencing
MM,
it
eccDNA
induced
amplifying
kinesin
family
member
3
C
(KIF3C)
reduce
MM.
Conclusion
Our
findings
indicated
regulated
alleviates
modulating
cuproptosis,
would
provide
novel
strategies
Pharmacological Research,
Год журнала:
2024,
Номер
202, С. 107144 - 107144
Опубликована: Март 13, 2024
Fibrosis
is
a
pathological
process
that
affects
multiple
organs
and
considered
one
of
the
major
causes
morbidity
mortality
in
diseases,
resulting
an
enormous
disease
burden.
Current
studies
have
focused
on
fibroblasts
myofibroblasts,
which
directly
lead
to
imbalance
generation
degradation
extracellular
matrix
(ECM).
In
recent
years,
increasing
number
role
epithelial
cells
fibrosis.
some
cases,
are
first
exposed
external
physicochemical
stimuli
may
drive
collagen
accumulation
mesenchyme.
other
source
stimulation
mainly
immune
cytokines,
similarly
altered
process.
this
review,
we
will
focus
dynamic
alterations
involved
after
injury
during
fibrogenesis,
discuss
association
among
them,
summarize
therapies
targeting
changed
cells.
Especially,
mesenchymal
transition
(EMT)
key
central
step,
closely
linked
biological
behaviors.
Meanwhile,
think
disruption
barrier,
cell
death
basal
stem
populations
stemness
fibrosis
not
appreciated.
We
believe
targeted
can
prevent
progress
fibrosis,
but
reverse
it.
The
provide
wonderful
preventive
delaying
action.
Journal of Materials Chemistry B,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
This
review
discusses
the
recent
developments
in
copper-based
nanomaterials
that
utilize
copper-induced
cell
death,
categorized
by
materials
systems,
while
highlighting
limitations
of
current
cuproptosis
related
nanomaterials.
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Июль 27, 2024
Abstract
Copper
is
an
important
metal
micronutrient,
required
for
the
balanced
growth
and
normal
physiological
functions
of
human
organism.
Copper-related
toxicity
dysbalanced
metabolism
were
associated
with
disruption
intracellular
respiration
development
various
diseases,
including
cancer.
Notably,
copper-induced
cell
death
was
defined
as
cuproptosis
which
also
observed
in
malignant
cells,
representing
attractive
anti-cancer
instrument.
Excess
copper
leads
to
aggregation
lipoylation
proteins
toxic
stress,
ultimately
resulting
activation
death.
Differential
expression
cuproptosis-related
genes
detected
tissues.
Cuproptosis-related
linked
regulation
oxidative
immune
responses,
composition
tumor
microenvironment.
Activation
increased
redox-metabolism-regulating
genes,
such
ferredoxin
1
(FDX1),
lipoic
acid
synthetase
(LIAS),
lipoyltransferase
(LIPT1),
dihydrolipoamide
dehydrogenase
(DLD),
drolipoamide
S-acetyltransferase
(DLAT),
pyruvate
E1
subunit
alpha
(PDHA1),
beta
(PDHB)).
Accordingly,
copper-activated
network
suggested
target
cancer
therapy.
Mechanisms
different
cancers
microenvironment
are
discussed
this
study.
The
analysis
current
findings
indicates
that
therapeutic
signaling,
targets
may
provide
effective
tool
improvement
immunotherapy
regimens.
Graphical
Journal of Inflammation Research,
Год журнала:
2025,
Номер
Volume 18, С. 4651 - 4664
Опубликована: Апрель 1, 2025
Oxygen
supplementation
is
essential
for
patients
with
a
multitude
of
diseases
but
can
cause
severe
hyperoxia-induced
lung
injury
(HLI),
necessitating
the
identification
therapeutic
targets
to
improve
clinical
outcomes.
Cuproptosis,
novel
copper-dependent
form
cell
death
characterized
by
proteotoxic
stress
resulting
from
lipoylated
protein
aggregation
and
loss
iron-sulfur
cluster
proteins,
distinct
other
forms
death.
However,
role
cuproptosis
in
HLI
remains
unclear.
We
established
an
model
MLE-12
cells
C57BL/6
mice
investigate
involvement
toxicity.
observed
time-dependent
increase
cuproptosis-related
gene
Fdx1
under
hyperoxia.
Moreover,
hyperoxia
activated
membrane-associated
copper
transporter
SLC31A1
significantly
elevated
levels
cells,
as
well
serum
tissue
mice.
Further
analysis
revealed
that
altered
expression
genes
without
affecting
DLAT
levels,
increased
lipoylated-DLAT
levels.
ELISA,
CCK-8
assays,
HE
staining,
wet-to-dry
weight
ratio,
bronchoalveolar
lavage
fluid
demonstrated
treatment
inhibitor
TTM
reduced
pro-inflammatory
cytokines
(TNF-α
IL-1β)
alleviated
both
Our
study
identifies
HLI,
providing
new
insights
into
pathogenesis
hyperoxic
potential
strategies.
International Journal of COPD,
Год журнала:
2025,
Номер
Volume 20, С. 1083 - 1096
Опубликована: Апрель 1, 2025
Chronic
obstructive
pulmonary
disease
(COPD)
is
a
leading
cause
of
death
worldwide,
and
its
pathogenesis
potentially
relevant
biomarkers
require
further
study.
Imbalance
in
copper
(Cu2+)
metabolism
related
to
series
diseases,
but
role
COPD
has
not
been
specified.
A
dataset
was
downloaded
from
Gene
Expression
Omnibus
database,
among
which
total
18
cuproptosis-related
genes
(CRGs)
were
screened.
The
SimDesign
package
used
perform
single-factor
Rogers
regression
screen
associated
with
phenotypes,
risk
score
prediction
models
constructed,
Receiver
Operating
Characteristic
(ROC)
curves
evaluate
the
efficacy
models.
In
addition,
we
verified
expression
CRGs
subtypes
correlation
between
clinical
characteristics
using
database.
Finally,
immune
analysis
explore
cell
infiltration.
Five
(DLST,
GLS,
LIPT1,
MTF1,
PDHB)
identified.
ROC
illustrated
that
these
five
performed
well
(area
under
curve
(AUCs)>0.7),
enrichment
scores
diagnostic
significantly
different
subtypes,
chi-square
test
P-values
age
groups
different.
infiltration
evaluation
cuproptosis
revealed
results
22
types
cells
showed
significant
cells,
correlated
content
most
cells.
four
pathways
differences
GSEA
Oxidative
Phosphorylation,
Parkinson's
Disease,
Purine
Metabolism,
Drug
Metabolism
Cytochrome
P450.
This
study
identified
candidate
for
investigation
constructed
pathways.
can
provide
basis
research
on
COPD.
