Piezo1-Induced Nasal Epithelial Barrier Dysfunction in Allergic Rhinitis DOI Creative Commons

Shengyang Liu,

Jianhua Wu, Meng Ling

и другие.

Inflammation, Год журнала: 2025, Номер unknown

Опубликована: Янв. 11, 2025

This study aimed to investigate the role of Piezo1 in nasal epithelial barrier dysfunction allergic rhinitis (AR) using both vitro and vivo experimental methods. A total 79 human mucosal samples were collected, including 43 from AR patients 36 healthy controls. Additionally, 12 BALB/c mice used for experiments. Human cells (HNEpCs) employed studies. In study, sensitized with ovalbumin (OVA) induce AR. experiments, expression HNEpCs was silenced shRNA, followed by stimulation IL-13. The Piezo1, ERK1/2, tight junctions (TJs) components (including ZO-1, Occludin, Claudin-1) assessed quantitative RT-PCR, immunofluorescence, Western blotting. Statistical analyses included paired Student's t-test one-way ANOVA. significantly elevated OVA-induced mice, while TJs reduced (p < 0.05). Knockdown restored levels improved integrity. negative correlation between ERK1/2 observed. plays a crucial modulating pathway. These findings suggest that may serve as potential therapeutic target

Язык: Английский

Piezo1-Induced Nasal Epithelial Barrier Dysfunction in Allergic Rhinitis DOI Creative Commons

Shengyang Liu,

Jianhua Wu, Meng Ling

и другие.

Inflammation, Год журнала: 2025, Номер unknown

Опубликована: Янв. 11, 2025

This study aimed to investigate the role of Piezo1 in nasal epithelial barrier dysfunction allergic rhinitis (AR) using both vitro and vivo experimental methods. A total 79 human mucosal samples were collected, including 43 from AR patients 36 healthy controls. Additionally, 12 BALB/c mice used for experiments. Human cells (HNEpCs) employed studies. In study, sensitized with ovalbumin (OVA) induce AR. experiments, expression HNEpCs was silenced shRNA, followed by stimulation IL-13. The Piezo1, ERK1/2, tight junctions (TJs) components (including ZO-1, Occludin, Claudin-1) assessed quantitative RT-PCR, immunofluorescence, Western blotting. Statistical analyses included paired Student's t-test one-way ANOVA. significantly elevated OVA-induced mice, while TJs reduced (p < 0.05). Knockdown restored levels improved integrity. negative correlation between ERK1/2 observed. plays a crucial modulating pathway. These findings suggest that may serve as potential therapeutic target

Язык: Английский

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