A macrophage subpopulation promotes airineme-mediated intercellular communication in a Matrix Metalloproteinase-9 dependent manner DOI Creative Commons
Raquel Lynn Bowman, Daoqin Wang, Dae Seok Eom

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Ноя. 28, 2022

Abstract Tissue-resident macrophages are highly heterogenous and perform various dedicated functions depending on their locations. In particular, skin resident have intriguing roles in long-distance intercellular signaling by mediating cellular protrusions called ‘airinemes’ zebrafish. During pigment pattern formation, relay molecules containing ‘airineme vesicles’ from one cell to another. Without macrophages, airineme-mediated is abolished, disrupting formation. It remains unknown, however, if the same macrophage population controls both these typical immune or a separate subpopulation communication. this study, with high-resolution confocal live-imaging type-specific genetic ablation approaches vivo , we identified responsible for signaling. These cells appear distinct conventional amoeboid morphology faster/expansive migratory behaviors. Instead, show that they resemble ectoderm-derived termed metaphocytes. Metaphocyte dramatically reduces airineme extension addition, amoeboid/metaphocytes require high levels of MMP9 expression migration results reveal novel specialized signaling, which may play many other aspects

Язык: Английский

Slik sculpts the plasma membrane into cytonemes to control cell-cell communication DOI Creative Commons
Basile Rambaud, Mathieu Joseph, Feng‐Ching Tsai

и другие.

The EMBO Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

Cytonemes are signaling filopodia that facilitate long-range cell-cell communication by forming synapses between cells. Initially discovered in Drosophila for transporting morphogens during embryogenesis, they have since been identified mammalian cells and implicated carcinogenesis. Despite their importance, mechanisms controlling cytoneme biogenesis remain elusive. Here, we demonstrate the Ser/Thr kinase Slik drives remote cell proliferation promoting formation. This function depends on coiled-coil domain of (SlikCCD), which directly sculpts membranes into tubules. Importantly, plays opposing roles biogenesis: its membrane-sculpting activity promotes formation, but this is counteracted activity, enhances actin association with plasma membrane via Moesin phosphorylation. In vivo, SlikCCD formation one epithelial layer wing disc to promote an adjacent layer. Finally, relies STRIPAK complex, controls governs at a distance regulating membrane. Our study unveils unexpected structural role sculpting membranes, crucial cytoneme-mediated control proliferation.

Язык: Английский

Процитировано

0
Making cells inter-connected for signaling communication: a developmental view of cytonemes DOI Creative Commons
Juntao Ma, Honglin Yu, Licheng Liu

и другие.

Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)

Опубликована: Май 25, 2025

Cellular communication is a cornerstone of metazoan development, orchestrating cell behavior, differentiation, and tissue formation. Morphogens, key signaling molecules for patterning architecture, are traditionally thought to act through diffusion or endocytosis but struggle explain precise long-range gradient formation in complex tissues. The discovery cytonemes, specialized actin-based membrane extensions, has introduced novel mechanism direct intercellular signaling. Their dynamic structure allows signaling, ensuring specificity accuracy morphogen delivery, which essential proper differentiation. In this review, we summarize the latest advances cytoneme research across different model organisms by focusing on regulatory mechanisms functional roles stem cells developmental disorders. We establish cytonemes as fundamental mediators emphasize their pivotal biology potential implications regenerative medicine cancer therapy.

Язык: Английский

Процитировано

0
The hidden hedgehog of the pituitary: hedgehog signaling in development, adulthood and disease of the hypothalamic-pituitary axis DOI Creative Commons
Yehan Bian, Heidi Hahn, Anja Uhmann

и другие.

Frontiers in Endocrinology, Год журнала: 2023, Номер 14

Опубликована: Июль 5, 2023

Hedgehog signaling plays pivotal roles in embryonic development, adult homeostasis and tumorigenesis. However, its engagement the pituitary gland has been long underestimated although embryogenic development are closely linked. Thus, deregulation of this pathway during results malformation gland. Research last years further implicates a regulatory role function pituitary, because activity is also interlinked with homeostasis, hormone production, most likely formation neoplasms The fact that can be efficiently targeted by validated therapeutic strategies makes it promising candidate for treating diseases. We here summarize current knowledge about importance review recent data highlight impact healthy diseased

Язык: Английский

Процитировано

5
A macrophage subpopulation promotes airineme-mediated intercellular communication in a matrix metalloproteinase-9 dependent manner DOI Creative Commons
Raquel Lynn Bowman, Daoqin Wang, Dae Seok Eom

и другие.

Cell Reports, Год журнала: 2023, Номер 42(7), С. 112818 - 112818

Опубликована: Июль 1, 2023

Tissue-resident macrophages are heterogeneous and perform location-dependent functions. Skin resident play intriguing roles in long-distance intercellular signaling by mediating cellular protrusions called airinemes zebrafish. These relay molecules containing airineme vesicles between pigment cells, their absence disrupts airineme-mediated pattern formation. It is unknown if the same control both these typical immune functions or a separate subpopulation communication. With high-resolution imaging genetic ablation approaches, we identify macrophage responsible for signaling. seem to be distinct from conventional skin-resident ameboid morphology faster expansive migratory behaviors. They resemble ectoderm-derived termed metaphocytes. Metaphocyte markedly decreases extension In addition, ameboid/metaphocytes require matrix metalloproteinase-9 migration results reveal with specialized signaling, which may other aspects of

Язык: Английский

Процитировано

5
Extracellular Vesicles and Membrane Protrusions in Developmental Signaling DOI Creative Commons
Callie M. Gustafson, Laura S. Gammill

Journal of Developmental Biology, Год журнала: 2022, Номер 10(4), С. 39 - 39

Опубликована: Сен. 21, 2022

During embryonic development, cells communicate with each other to determine cell fate, guide migration, and shape morphogenesis. While the relevant secreted factors their downstream target genes have been characterized extensively, how these signals travel between is still emerging. Evidence accumulating that extracellular vesicles (EVs), which are well defined in culture cancer, offer a crucial means of communication embryos. Moreover, release and/or reception EVs often facilitated by fine cellular protrusions, history study development. However, due part complexities identifying fragile nanometer-scale structures within three-dimensional environment, nomenclature developmental protrusions can be ambiguous, confounding progress. In this review, we provide robust categorizing order enable comparisons systems stages. Then, discuss existing evidence supporting role for throughout

Язык: Английский

Процитировано

8
Cytoneme-mediated intercellular signaling in keratinocytes essential for epidermal remodeling DOI Creative Commons
Yi Wang, Thomas Nguyen,

Qingan He

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 12, 2023

The skin, the largest organ, functions as a primary defense mechanism. Epidermal stem cells supply undifferentiated keratinocytes that differentiate they migrate toward outermost skin layer. Although such replenishment process is disrupted in various human diseases, its underlying mechanisms remain elusive. With high-resolution live imaging and vivo manipulations, we revealed Notch signaling between mediated by filopodia called cytonemes essential for proper keratinocyte differentiation proliferation. Inhibiting reduced expression within keratinocytes, leading to abnormal hyperproliferation, resembling disease phenotypes. Overproduction of Interleukin (IL)-17 signal, associated with diseases like psoriasis, induces psoriatic phenotypes via zebrafish. Our study suggests intercellular through critical epidermal maintenance, misregulation could be an origin diseases.

Язык: Английский

Процитировано

1
CD44 facilitates adhesive interactions in airineme-mediated intercellular signaling DOI Creative Commons
Raquel Lynn Bowman, Jiyea Kim, Dae Seok Eom

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 28, 2024

Specialized cellular protrusions facilitate local intercellular communications in various species, including mammals. Among these, airinemes play a crucial role pigment pattern formation zebrafish by mediating long-distance Notch signaling between cells. Remarkably, exhibit large vesicle-like structure at their tips, which are pulled macrophages and delivered to target The interaction Delta-ligand carrying airineme vesicles is essential for initiating airineme-mediated signaling, yet the molecular detail of this remains elusive. Through high-resolution live imaging, genetic

Язык: Английский

Процитировано

0
STRIPAK controls cell-cell communication by promoting cytoneme biogenesis through the membrane-sculpting function of Slik DOI Open Access
Basile Rambaud, Mathieu Joseph, Feng‐Ching Tsai

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 6, 2024

ABSTRACT Cytonemes are signaling filopodia that facilitate long-range cell-cell communication by forming synapses between cells. Initially discovered in Drosophila for transporting morphogens during embryogenesis, they have since been identified mammalian cells and recently implicated carcinogenesis. Yet, despite their importance, the mechanisms controlling cytoneme biogenesis remain elusive. Here, we demonstrate Ser/Thr kinase Slik drives remote cell proliferation promoting formation. We this function depends on coiled-coil domain of (Slik CCD ), which directly sculpts membranes into tubules. Importantly, plays paradoxical roles biogenesis. While its membrane-sculpting activity promotes formation, it is counteracted activity, enhances actin association with plasma membrane via Moesin phosphorylation. In vivo , formation cytonemes one epithelial layer wing disc to promote an adjacent layer. Finally, found relies STRIPAK complex, controls governs at a distance regulating membrane. Our study unveils first family kinases membranes, crucial cytoneme-mediated control proliferation.

Язык: Английский

Процитировано

0
Cytoneme-mediated intercellular signaling in keratinocytes essential for epidermal remodeling DOI Open Access
Yi Wang,

Qingan He,

Oliver Has

и другие.

Опубликована: Май 16, 2024

The skin, the largest organ, functions as a primary defense mechanism. Epidermal stem cells supply undifferentiated keratinocytes that differentiate they migrate toward outermost skin layer. Although such replenishment process is disrupted in various human diseases, its underlying mechanisms remain elusive. With high-resolution live imaging and vivo manipulations, we revealed Notch signaling between mediated by filopodia called cytonemes essential for proper keratinocyte differentiation proliferation. Inhibiting reduced expression within keratinocytes, leading to abnormal hyperproliferation, resembling disease phenotypes. Overproduction of Interleukin (IL)-17 signal, associated with diseases like psoriasis , induces psoriatic phenotypes via zebrafish. Our study suggests intercellular through critical epidermal maintenance, misregulation could be an origin diseases.

Язык: Английский

Процитировано

0
Cytoneme-mediated intercellular signaling in keratinocytes essential for epidermal remodeling DOI Open Access
Yi Wang,

Qingan He,

Oliver Has

и другие.

Опубликована: Май 16, 2024

The skin, the largest organ, functions as a primary defense mechanism. Epidermal stem cells supply undifferentiated keratinocytes that differentiate they migrate toward outermost skin layer. Although such replenishment process is disrupted in various human diseases, its underlying mechanisms remain elusive. With high-resolution live imaging and vivo manipulations, we revealed Notch signaling between mediated by filopodia called cytonemes essential for proper keratinocyte differentiation proliferation. Inhibiting reduced expression within keratinocytes, leading to abnormal hyperproliferation, resembling disease phenotypes. Overproduction of Interleukin (IL)-17 signal, associated with diseases like psoriasis , induces psoriatic phenotypes via zebrafish. Our study suggests intercellular through critical epidermal maintenance, misregulation could be an origin diseases.

Язык: Английский

Процитировано

0