From stem cells to pancreatic β-cells: strategies, applications, and potential treatments for diabetes
Molecular and Cellular Biochemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 20, 2024
Язык: Английский
Comprehensive Characterization of Bihormonal Cells and Endocrine Cell Lineages in Mammalian Pancreatic Islets
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 29, 2025
Abstract
Understanding
the
role
and
prevalence
of
bihormonal
cells
in
pancreatic
islets
their
potential
β‐cell
restoration
is
critical
but
remains
ambiguous.
Using
genetically
engineered
mouse
strains
with
specific
fluorescent
markers
advanced
imaging
flow
cytometry,
it
found
that
are
exceedingly
rare.
Single‐cell
RNA
sequencing
reveals
Gcg
+
Ppy
Ins
closely
resemble
α‐cells
or
PP‐cells
α‐cells,
respectively,
indicating
they
neither
unique
lineages
nor
transitional
states.
Dual‐recombinase
lineage
tracing
further
demonstrates
embryonic
resolve
into
monohormonal
α‐cells.
Applying
these
insights,
scarcity
diabetic
models
confirmed,
suggesting
a
limited
regeneration.
By
excluding
influences,
endocrine
cell
classification
redefined
human
through
gene
coexpression
network
analysis,
identifying
distinct
subtypes
regulatory
modules
while
uncovering
species‐specific
differences.
Additionally,
two
δ‐cell
subpopulations
identified
islets.
Collectively,
this
study
provides
comprehensive
characterization
cells,
refines
taxonomy,
underscores
translational
challenges
modeling
islet
biology
mice.
Язык: Английский
A systems view of cellular heterogeneity: Unlocking the “wheel of fate”
Hourieh Movasat,
Enzo Giacopino,
Ali Shahdoost
и другие.
Cell Systems,
Год журнала:
2025,
Номер
unknown, С. 101300 - 101300
Опубликована: Июнь 1, 2025
Язык: Английский
Exendin-4 affects calcium signalling predominantly during activation and activity of beta cell networks in acute mouse pancreas tissue slices
Frontiers in Endocrinology,
Год журнала:
2024,
Номер
14
Опубликована: Янв. 16, 2024
Tight
control
of
beta
cell
stimulus-secretion
coupling
is
crucial
for
maintaining
homeostasis
energy-rich
nutrients.
While
glucose
serves
as
a
primary
regulator
this
process,
incretins
augment
function,
partly
by
enhancing
cytosolic
[Ca
2+
]
dynamics.
However,
the
details
how
precisely
they
affect
recruitment
during
activation,
their
active
time,
and
functional
connectivity
plateau
activity,
influence
deactivation
remain
to
be
described.
Performing
multicellular
Ca
imaging
in
acute
mouse
pancreas
tissue
slices
enabled
us
systematically
assess
effects
GLP-1
receptor
agonist
exendin-4
(Ex-4)
simultaneously
many
coupled
cells
with
high
resolution.
In
otherwise
substimulatory
glucose,
Ex-4
was
able
recruit
approximately
quarter
into
an
state.
Costimulation
stimulatory
shortened
activation
delays
accelerated
More
specifically,
time
increased
faster,
required
reach
half-maximal
effectively
halved
presence
Ex-4.
Moreover,
regularity
IC
oscillations
increased,
especially
first
part
response.
contrast,
subsequent
addition
already
did
not
significantly
enhance
activity.
Network
analyses
further
confirmed
activity
Ex-4,
hub
roles
remaining
rather
stable
both
experiments
Interestingly,
demonstrated
biphasic
effect
on
deactivation,
slightly
prolonging
at
physiological
concentrations
shortening
supraphysiological
concentrations.
sum,
costimulation
increases
indicating
that
may,
important
extent,
explained
enhanced
signals.
During
previous
incretin
stimulation
does
critically
prolong
cellular
which
corroborates
low
risk
hypoglycemia.
Язык: Английский
Recent advancements in lipid nanoparticle technology for oral insulin delivery
University of Colombo Review,
Год журнала:
2024,
Номер
5(1), С. 130 - 144
Опубликована: Июнь 18, 2024
Nanotechnology
is
important
in
many
industries,
particularly
drug
delivery
and
administration.
This
technology
has
been
used
to
create
nanocarriers
for
enteral
delivery.
There
are
different
types
of
including
polysaccharide-based
lipid-based
nanocarriers.
Lipid
nanoparticles
have
recently
developed
loading
administering
insulin
via
the
route
treat
diabetes
mellitus.
Insulin
cannot
be
administered
orally
due
its
low
bioavailability,
extreme
gastrointestinal
conditions,
instability.
When
loaded
onto
these
nanocarriers,
it
increases
stability,
release
pattern
control,
protects
from
conditions.
review
article
aims
discuss
recent
applications
as
well
novel
methods
approaches
oral
using
liposomes,
solid
lipid
nanoparticles,
nanostructured
carriers.
Язык: Английский
An INSULIN and IAPP dual reporter enables tracking of functional maturation of stem cell-derived insulin producing cells
Carmen Bayly,
Xiao-Qing Dai,
Cuilan Nian
и другие.
Molecular Metabolism,
Год журнала:
2024,
Номер
89, С. 102017 - 102017
Опубликована: Авг. 24, 2024
Human
embryonic
stem
cell
(hESC;
SC)-derived
pancreatic
β
cells
can
be
used
to
study
diabetes
pathologies
and
develop
replacement
therapies.
Although
current
differentiation
protocols
yield
SCβ
with
varying
degrees
of
maturation,
these
still
differ
from
deceased
donor
human
in
several
respects.
We
sought
a
reporter
line
that
could
dynamically
track
functional
maturation.
Язык: Английский
Tipping-point transition from transient to persistent inflammation in pancreatic islets
npj Systems Biology and Applications,
Год журнала:
2024,
Номер
10(1)
Опубликована: Сен. 12, 2024
Язык: Английский
Lantidra: Cell Therapy for Treatment of Type 1 Diabetes Mellitus
Shital B. Bharambe,
Shailesh Jawarkar
Asian Journal of Pharmaceutical Research,
Год журнала:
2024,
Номер
unknown, С. 331 - 335
Опубликована: Сен. 20, 2024
The
more
recent
cell
treatment
for
type
1
Diabetes
is
called
lantidra.
Lantidra
has
recently
received
approval
to
treat
mellitus.
first
allogeneic
(deceased
donor)
pancreatic
islet
therapy,
lantidra
(donislecel),
was
approved
by
the
FDA
Centre
Biologics
Evaluation
and
Research
(CBER)
on
June
28,
2023.
It
intended
adults
with
diabetes
who,
despite
current
management,
do
not
achieve
target
glycosylated
hemoglobin
levels
due
recurrent
episodes
of
severe
hypoglycemia.
editorial’s
goal
draw
attention
growing
impact
world’s
health,
discuss
earlier
attempts
at
transplant
techniques,
announces
regulatory
a
revolutionary
strategy
beta
infusion.
Язык: Английский