Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 289, С. 117466 - 117466
Опубликована: Дек. 7, 2024
Язык: Английский
Industrial Crops and Products, Год журнала: 2025, Номер 226, С. 120622 - 120622
Опубликована: Фев. 6, 2025
Язык: Английский
Процитировано
0
BMC Plant Biology, Год журнала: 2024, Номер 24(1)
Опубликована: Янв. 16, 2024
Abstract Background MYB transcription factors are splay a vital role in plant biology, with previous research highlighting the significant impact of R2R3-MYB-like factor MYB5 on seed mucilage biosynthesis, trichome branching, and coat development. However, there is dearth studies investigating its regulation proanthocyanidin (PA) biosynthesis. Results In this study, total 51 homologous genes were identified across 31 species belonging to Brassicaceae family, particular emphasis Brassica napus for subsequent investigation. Through phylogenetic analysis, these categorized into four distinct subclasses. Protein sequence similarity identity analysis demonstrated high degree conservation among within family. Additionally, examination selection pressure revealed that predominantly underwent purifying during evolutionary history, as indicated by Ka/Ks values all gene pairs being less than one. Notably, we observed higher rate non-synonymous mutations orthologous compared paralogous genes, value displayed stronger correlation Ka. B. , an expression patterns five tissues exhibited particularly black coat. The findings from WGCNA robust between BAN ( ANR ) associated PA biosynthesis coat, providing further evidence their close association co-expression. Furthermore, results obtained protein interaction networks offer supplementary support proposition possesses capability interact transcriptional regulatory proteins, specifically TT8 TT2 alongside catalytic enzymes implicated synthesis PAs, thereby making contribution PAs. These imply plausible nuique pattern pigmentation rapeseed coats. Nevertheless, additional endeavors imperative authenticate substantiate findings. Conclusions This study offers valuable insights genetic evolution plants, serving reference enhancement color.
Язык: Английский
Процитировано
2
Plants, Год журнала: 2024, Номер 13(6), С. 760 - 760
Опубликована: Март 7, 2024
α-Linolenic acid (ALA) is an important nutrient component in rapeseed oil, and breeders want to either restrain or enhance the function of fatty desaturases (FADs) ALA biosynthesis pathway. To determine reason for upregulation BnFAD genes two high-ALA accessions, R8Q10 YH25005, we compared their transcriptome profiles seed at 24 days after pollination (DAP) with those low-ALA lines, A28 SW. The expression levels twenty-eight samples 20, 27, 34 DAP were also investigated using RT-qPCR. involved flavonoid proanthocyanidin synthesis, including BnCHS, BnCHI, BnDFR, BnFLS1, BnLDOX, BnBAN, BnTT10, BnTT12 encoding transcription factors BnTT1, BnTT2, BnTT8, BnTT16 lower YH25005 than master embryo development, such as BnLEC1, BnABI3, BnFUS3, BnL1L, BnAREB3, BnbZIP67, elevated significantly accessions. Combined previous results Arabidopsis literature, speculated that yellow-seededness could elevate activity etc., by reducing several transparent testa homologs, resulting BnFAD3 BnFAD7 acceleration synthesis. Yellow-seededness a favorable factor promote synthesis accessions yellow-seeded trait. These findings provide initial insights into transcriptomic differences between high-/low-ALA germplasms theoretic basis quality breeding.
Язык: Английский
Процитировано
1
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(24), С. 17368 - 17368
Опубликована: Дек. 11, 2023
Tartary buckwheat (Fagopyrum tataricum) is an important plant, utilized for both medicine and food. It has become a current research hotspot due to its rich content of flavonoids, which are beneficial human health. Anthocyanins (ATs) proanthocyanidins (PAs) the two main kinds flavonoid compounds in buckwheat, participate pigmentation some tissue as well rendering resistance many biotic abiotic stresses. Additionally, anthocyanins PAs have health benefits humans plant itself. However, little known about regulation mechanism biosynthesis anthocyanin PA buckwheat. In present study, bHLH transcription factor (TF) FtTT8 was characterized be homologous with AtTT8 phylogenetically close proteins from other species. Subcellular location yeast two-hybrid assays suggested that locates nucleus plays role factor. Complementation analysis Arabidopsis tt8 mutant showed could not recover deficiency but promote accumulation. Overexpression red-flowering tobacco inhibits accelerates proanthocyanidin biosynthesis. QRT-PCR one-hybrid assay revealed might bind promoter NtUFGT suppress expression, while binding NtLAR upregulating expression K326 tobacco. This displayed bidirectional regulating function negatively regulates positively The results provide new insights on TT8 inconsistent species, high-quality gene resource breeding.
Язык: Английский
Процитировано
3
Frontiers in Plant Science, Год журнала: 2023, Номер 14
Опубликована: Ноя. 23, 2023
In recent years, numerous genes that encode proteins with specific domains participate in different biological processes or have molecular functions been identified. A class of typical whose function has rarely identified and another type no attracted increasing attentions. As many these so-called as unknown/uncharacterized (U/U) are involved important processes, such plant growth stress resistance, there is much interest deciphering their roles. Here, we summarize our current understanding genes, including structures, classifications, roles progress the methods used to decipher provide new research perspectives. Unveiling may suggest strategies fine-tune physiological plants, which will enrich functional network system plants more possibilities for adaptive improvement plants.
Язык: Английский
Процитировано
2
Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 289, С. 117466 - 117466
Опубликована: Дек. 7, 2024
Язык: Английский
Процитировано
0