Theoretical and Applied Genetics, Год журнала: 2024, Номер 137(12)
Опубликована: Ноя. 21, 2024
Язык: Английский
The Plant Genome, Год журнала: 2025, Номер 18(1)
Опубликована: Янв. 5, 2025
Abstract Leaf rust, caused by Puccinia triticina ( Pt ), is a serious constraint to wheat production. Developing resistant varieties the best approach managing this disease. Wheat leaf rust resistance Lr ) genes have been classified into either all‐stage (ASR) or adult‐plant (APR). The objectives of study were identify sources in contemporary US hard winter (HWW) and dissect genetic basis underlying HWW. A panel 732 elite HWW genotypes was evaluated for response races at seedling stage adult plant nurseries Oklahoma, Texas, Kansas. Further, genotyped using multiplex restriction amplicon sequencing (MRA‐Seq) DNA markers linked known ASR Lr18 , Lr19 Lr21 Lr24 Lr37 Lr42 APR Lr34 Lr46 Lr67 Lr68 Lr77 Lr78 . Single nucleotide polymorphism (SNP) derived from MRA‐Seq, genes, phenotypic data used genome‐wide association (GWAS) associated with response. Gene postulation based on reactions, markers, GWAS suggested presence Lr1 Lr2a Lr10 Lr14a Lr16 Lr26 Lr39 panel. identified 59 SNPs significantly response, which 20 likely novel loci can be enhance resistance.
Язык: Английский
Процитировано
1
Plants, Год журнала: 2025, Номер 14(7), С. 1146 - 1146
Опубликована: Апрель 7, 2025
In an effort to enhance wheat's resilience against rust diseases, our research explores the genetic underpinnings of resistance in a diverse collection winter bread wheat accessions. Leaf (Puccinia triticina), yellow striiformis f. sp. tritici), and stem graminis tritici) are significant threats global production. By leveraging host resistance, we can improve disease management strategies. Our study evaluated 55 accessions, including germplasm from Kazakhstan, Uzbekistan, Russia, Kyrgyzstan, France, CIMMYT under field conditions southern Kazakhstan 2022 2024. The results showed robust profile: 49.1% accessions exhibited high moderate leaf rust, 12.7% 30.9% rust. Notably, ten demonstrated multiple species, while seven two rusts. Twenty were selected for further seedling molecular analysis. Three proved resistant six isolates P. triticina, four striiformis, five graminis. Although no genotypes found be universally all species at stage, accessions-Bezostaya 100 (Russia) KIZ 90 (Kazakhstan)-displayed consistent both evaluations. Molecular analysis revealed presence key genes, Lr1, Lr3, Lr26, Lr34, Yr9, Yr18, Sr31, Sr57, 1AL.1RS translocation. This work provides valuable insights into landscape contributes development new cultivars that withstand these enhancing food security.
Язык: Английский
Процитировано
0
Frontiers in Genome Editing, Год журнала: 2025, Номер 7
Опубликована: Фев. 25, 2025
Wheat is cultivated across diverse global environments, and its productivity significantly impacted by various biotic stresses, most importantly but not limited to rust diseases, Fusarium head blight, wheat blast, powdery mildew. The genetic diversity of modern cultivars has been eroded domestication selection, increasing their vulnerability stress due uniformity. rapid spread new highly virulent aggressive pathogen strains exacerbated this situation. Three strategies can be used for enhancing disease resistance through genome editing: introducing (R) gene-mediated resistance, engineering nucleotide-binding leucine-rich repeat receptors (NLRs), manipulating susceptibility (S) genes stop pathogens from exploiting these factors support infection. Utilizing R the common strategy traditional breeding approaches, continuous evolution effectors eventually overcome resistance. Moreover, modifying S confer pleiotropic effects that hinder use in agriculture. Enhancing paramount sustainable production food security, tools are great importance research community. application CRISPR-based editing provides promise improve allowing access a broader range solutions beyond random mutagenesis or intraspecific variation, unlocking ways crops, speeding up breeding. Here, we first summarize major context important diseases limitations. Next, turn our attention powerful applications technology creating varieties against diseases.
Язык: Английский
Процитировано
0
Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)
Опубликована: Март 10, 2025
Abstract Key message The GWAS and testing with Yr gene linked markers identified 109 loci including 40 novel for all-stage adult plant stage resistance to stripe rust in 459 US contemporary hard winter wheat genotypes. Stripe is a destructive disease, caused by Puccinia striiformis f. sp. tritici ( Pst ). To identify sources of wheat, panel Great Plains genotypes was evaluated at the seedling against five races field environments Oklahoma, Kansas, Washington. results showed that 7–14% were resistant stage, whereas 32–78% across environments, indicating presence resistance. Sixteen displayed broad spectrum all environments. further genotyped using 9858 single-nucleotide polymorphisms (SNPs) generated from multiplex restriction amplicon sequencing (MRASeq) functional DNA known ) genes Yr5 , Yr15 Yr17 Yr18 Yr29 Yr36 Yr40 Yr46 QYr.tamu-2B . A genome-wide association study (GWAS) performed genotypic phenotypic data, which 110 SNPs be significantly associated response. In addition, detected their panel. This genomic regions not previously characterized genes. These findings offer significant opportunities diversify enhance wheat.
Язык: Английский
Процитировано
0
Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)
Опубликована: Март 13, 2025
Abstract Key message Genetic variation in stripe rust resistance exists Australian wheat breeding populations and is environmentally influenced. Stacking multiple haplotypes or using whole-genome approaches will improve stability environmental specificity. Wheat ( Puccinia striiformis ) a fungal disease responsible for substantial yield losses globally. To maintain crop productivity future climates, the identification of genetics offering durable across diverse growing conditions crucial. stay one-step ahead pathogen, breeders are actively selecting adult-plant (APR), which considered more than seedling resistance. However, deploying that stable effective environments years challenging as expression underling APR loci often interacts with conditions. explore underlying interactions environment resistance, we employ haplotype-based mapping local GEBV approach elite populations. Our multi-environment trial analyses comprising 35,986 inbred lines evaluated 10 revealed significant genotype-by-environment rust. A total 32 haploblocks associated were identified, where 23 unique to specific nine environments. Population structure analysis commercial advanced carried desirable haplotypes, highlighting opportunity continue harness optimise already present within backgrounds. Further, demonstrate silico stacking through has potential substantially levels. This represents largest study date exploring highlights opportunities target
Язык: Английский
Процитировано
0
BMC Plant Biology, Год журнала: 2025, Номер 25(1)
Опубликована: Март 17, 2025
Язык: Английский
Процитировано
0
PLoS ONE, Год журнала: 2024, Номер 19(9), С. e0309944 - e0309944
Опубликована: Сен. 6, 2024
Leaf rust caused by Puccinia triticina (Pt) is one of the most impactful diseases causing substantial losses in common wheat (Triticum aestivum L.) crops. In adult plants resistant to Pt, a horizontal plant resistance (APR) observed: APR protects against multiple pathogen races and distinguished durable persistence under production conditions. The Lr46/Yr29 locus was mapped chromosome 1B genome, but identity underlying gene has not been demonstrated although several candidate genes have proposed. This study aimed analyze expression nine located at their four complementary miRNAs (tae-miR5384-3p, tae-miR9780, tae-miR9775, tae-miR164), response Pt infection. materials tested included five reference cultivars which molecular marker csLV46G22 associated with Lr46/Yr29-based identified, as well susceptible control cultivar. Biotic stress induced inoculation fungal spores controlled Plant material sampled before 6, 12, 24, 48 hours post (hpi). Differences were analyzed qRT-PCR showed that varied time points. highest (Lr46-Glu1, Lr46-Glu2, Lr46-Glu3, Lr46-RLK1, Lr46-RLK2, Lr46-RLK3, Lr46-RLK4, Lr46-Snex, Lr46-WRKY) occurred 12 24 hpi such profiles obtained only for among (Lr46-Glu2), indicating it may be contributing factor
Язык: Английский
Процитировано
2
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Окт. 6, 2024
Abstract Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is a significant threat to global wheat production. Genetic resistance plays crucial role in controlling this disease. Among breeding innovations, the wheat-rye 1BL.1RS translocations are notable for introducing alien genetic diversity, partly due presence of rust gene Yr9 on 1RS. To clone , we first identified four -susceptible mutants from Lumai 15, which carries translocation and . Using these mutants, performed Sequencing Trait-Associated Mutations (STAM). A single candidate gene, YrChr1B was within locus later confirmed as through complementation editing. The encodes coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR or NLR) protein part 14-member NLR cluster. This cluster conserved among Triticeae species an ortholog barley Mla locus. Cloning expands resources available molecular aimed at durable broad-spectrum disease resistance.
Язык: Английский
Процитировано
2
Theoretical and Applied Genetics, Год журнала: 2024, Номер 137(9)
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
1
Plants, Год журнала: 2024, Номер 13(17), С. 2502 - 2502
Опубликована: Сен. 6, 2024
Wheat rusts, including leaf, stripe, and stem rust, have been a threat to global food security due their devastating impact on wheat yields. In recent years, significant strides made in understanding focusing disease spread mechanisms, the discovery of new host resistance genes, molecular basis rust pathogenesis. This review summarizes latest approaches studies research that provide comprehensive mechanisms insights into control strategies. Recent advances genetic using modern genomics techniques, as well pathogenesis resistance, are discussed. addition, innovative management strategies, use fungicides biological agents, reviewed, highlighting role combating rust. also emphasizes climate change epidemiology underscores importance developing resistant varieties along with adaptive practices. Finally, gaps knowledge identified suggestions for future made. aims inform researchers, agronomists, policy makers, contribute development more effective sustainable
Язык: Английский
Процитировано
1