Rapid Determination of Cellulose and Starch in Tobacco by Colorimetric Method
ACS Omega,
Год журнала:
2025,
Номер
10(13), С. 13548 - 13554
Опубликована: Март 27, 2025
Starch
and
cellulose
are
pivotal
determinants
of
tobacco
quality.
In
this
work,
starch
in
flue-cured
leaf
were
extracted
through
a
sequential
extraction
process.
First,
an
aqueous
solution
leaves
was
prepared,
by
boiling
the
water.
The
precipitate
separated,
within
it
hydrolyzed
using
sulfuric
acid
solution.
Then,
content
quantified
colorimetric
reaction
with
iodine
solution,
while
determined
anthrone
as
chromogenic
agent.
conditions
optimized
to
ensure
accuracy,
recovery
rate
relative
standard
deviation
(RSD)
calculated
under
these
optimum
conditions.
rates
for
samples
ranged
from
90
105%,
RSD
interday
intraday
determination
being
less
than
5%.
These
results
demonstrate
that
proposed
method
is
both
sensitive
reproducible,
making
suitable
rapid
precise
quantification
samples.
Язык: Английский
Influence of specific tobacco endophytic Bacillus on tobacco leaf quality enhancement during fermentation
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 25, 2024
Introduction
This
study
aimed
to
investigate
the
potential
role
of
endophytic
bacteria
in
tobacco
leaves
during
fermentation
process
enhance
quality
tobacco.
Methods
We
isolated
11
from
fresh
and
selected
Bacillus
halotolerans
NS36
mycoides
NS75
based
on
sensory
evaluation,
both
which
significantly
improved
leaves.
Results
Specifically,
decreased
offensive
taste
leaves,
while
by
increasing
aroma.
Chemical
analysis
revealed
that
with
B.
content
irritant
compounds
such
as
lignin,
cellulose,
starch,
pectin.
In
contrast,
reduced
protein,
Amadori
glycosides.
Through
whole-genome
sequencing,
we
predicted
enzyme
systems
related
these
chemical
changes.
mainly
secreted
associated
degradation
pectin,
thereby
reducing
irritants
diminishing
unpleasant
tastes,
achieving
a
more
balanced
quality.
NS75,
other
hand,
protein
glycoside
hydrolysis,
Maillard
reaction
products
glycosylated
thus
enhancing
aroma
quantity.
Discussion
The
findings
this
offer
new
perspective
for
industry,
namely,
use
bacilli
improve
off-flavors
could
not
only
industrial
applicability
but
also
potentially
strengthen
market
competitiveness
products.
These
discoveries
lay
foundation
further
research
application,
especially
development
biotechnologies
Язык: Английский
Co-production of sugars and aroma compounds from tobacco waste using biomass-degrading enzymes produced by Aspergillus brunneoviolaceus Ab-10
Archives of Microbiology,
Год журнала:
2024,
Номер
206(7)
Опубликована: Июнь 7, 2024
Язык: Английский
Analysis of the effects of Bacillus velezensis HJ-16 inoculation on tobacco leaves based on multi-omics methods
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Дек. 6, 2024
In
this
study,
a
strain
isolated
from
the
surface
of
flue-cured
tobacco
leaves,
identified
as
Язык: Английский