Lactic acid bacteria: Nature, Characterization, Mode of action, Products and Applications

Process Biochemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

---

Bacteriocins in Cancer Treatment: Mechanisms and Clinical Potentials

Biomolecules, Год журнала: 2024, Номер 14(7), С. 831 - 831

Опубликована: Июль 10, 2024

Cancer poses a severe threat to human health. Although conventional chemotherapy remains cornerstone of cancer treatment, its significant side effects and the growing issue drug resistance necessitate urgent search for more efficient less toxic anticancer drugs. In recent years, bacteriocins, antimicrobial peptides microbial origin, have garnered attention due their targeted antitumor activity. This unique activity is mainly attributed cationic amphiphilic nature, which enables bacteriocins specifically kill tumor cells without harming normal cells. When involving non-membrane-disrupting mechanisms, such as apoptosis induction, cell cycle blockade, metastasis inhibition, core mechanism action achieved by disrupting membranes, endows with low high selectivity. However, susceptibility hydrolysis hemolysis in vivo limits clinical application. To overcome these challenges, structural optimization or combination nanotechnology proposed future development. review aims study current research status treatments, thus providing new insights development

Язык: Английский

---

Bacteriocins from lactic acid bacteria: strategies for the bioprotection of dairy foods

Frontiers in Food Science and Technology, Год журнала: 2024, Номер 4

Опубликована: Авг. 20, 2024

Lactic acid bacteria (LAB), essential in fermenting milk, produce an array of antimicrobial compounds, notably bacteriocins, which contribute to the extended shelf life dairy goods. Bacteriocins, ribosomally synthesized peptides, display broad or narrow-spectrum activity, thus holding promise food preservation. The classification LAB bacteriocins is intricate, reflecting evolving genomic insights and biosynthesis mechanisms. Strategies for integrating into products include purified forms, bacteriocin-producing LAB, bacteriocin-containing fermentates, each with distinct advantages considerations. Optimization fermentation conditions, encompassing time, temperature, pH, culture medium, maximizing bacteriocin production. This optimization facilitates enhanced quality safety fermented items, aligning growing consumer preference natural, minimally processed foods. Furthermore, incorporation a hurdle approach alongside thermal non-thermal treatments holds augmenting bioprotection while reducing reliance on chemical preservatives. comprehensive overview underscores potential as effective alternative conventional preservatives, offering their application product

Язык: Английский

---

A gut-derived Streptococcus salivarius produces the novel nisin variant designated nisin G and inhibits Fusobacterium nucleatum in a model of the human distal colon microbiome

mBio, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

is a human pathogen associated with intestinal conditions including colorectal cancer. Screening for gut-derived strains that exhibit anti-

Язык: Английский

---

In Vitro Insights into Bacteriocin-Mediated Modulation of Chicken Cecal Microbiota

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 755 - 755

Опубликована: Янв. 17, 2025

Reducing the use of antibiotics in animal husbandry is essential to limit spread resistance. A promising alternative resides bacteriocins, which are antimicrobial peptides produced by bacteria showing a great diversity terms spectrum activity, structure, and mechanism action. In this study, effects diverse bacteriocins on composition metabolic activity chicken cecal microbiota were examined vitro, comparison with antibiotics. Different impacts revealed 16S metabarcoding, colistin having most dramatic impact diversity. Bacteriocins Gram-negative bacteria, microcins J25 E492, did not significantly influence composition. contrast, from Gram-positive impacted abundance lactic acid nisin Z while pediocin PA-1 (M31L) exhibited moderate effect at highest concentration tested. This study emphasizes potential as alternatives poultry protect pathogens such Salmonella, Clostridium, Enterococcus.

Язык: Английский

---

The potential of Streptococcus pyogenes and Escherichia coli bacteriocins in synergistic control of Staphylococcus aureus

Preparative Biochemistry & Biotechnology, Год журнала: 2025, Номер unknown, С. 1 - 9

Опубликована: Янв. 28, 2025

Staphylococcus aureus has developed resistance to most conventional antibiotics and is a causative agent of serious infections. Alternative therapies are urgently needed. Bacteriocins ribosomally synthesized antimicrobial peptides produced by bacteria, including Escherichia coli (E. coli) Streptococcus pyogenes (S. pyogenes), represent potential solution. While several bacteriocins have shown promise, their synergy with from other bacterial species remains largely unexplored. This work used agar diffusion on Muller-Hinton Agar (MHA) S. as test bacterium evaluate E. coli, combined bacteriocins. The showed the maximum activity zone inhibition (ZOI), 24.93 mm, compared that bacteriocin, which was 19.28 ones at 100% concentration, 22.6 mm. 50% concentration reduced 18.35 These observations suggest higher specificity against aureus, making them effective therapeutic agents in fight multidrug-resistant

Язык: Английский

---

The nisin O cluster: species dissemination, candidate leader peptide proteases and the role of regulatory systems

Microbiology, Год журнала: 2025, Номер 171(2)

Опубликована: Фев. 10, 2025

Nisin O is an antimicrobial peptide encoded by the human gut bacterium Blautia obeum A2-162 which has activity against clinically relevant organisms. The nisin biosynthetic gene cluster (BGC) varies from other BGCs as it lacks a leader-peptide cleaving protease and contains two bacterial two-component response regulator–histidine kinase (RK) systems. dissemination of cluster, final proteolytic biosynthesis step regulation are currently unknown foci this study. We identified six O-like across , Dorea Ruminococcus species using comparative genomics. These show evidence genetic transfer between genera, with genes involved in transposition discovered up- downstream BGCs. All contained RK systems but no protease. Mining B. genome candidate proteases that were cloned used pre-nisin leader cleavage assays. None removed leader; however, was achieved trypsin. To maximize expression nsoA1-4 peptides, interactions predicted promoters assessed PepI reporter assay. observed P nsoR2K2 promoter constitutively expressed, NsoR1K1 increasing its activity, there increased when A system present. Long-read cDNA sequencing confirmed nso transcription heterologous novel, highly expressed gene. This study provides BGC been transferred different gut-associated all clusters lacking containing hypothesize lost due to negative selection result high trypsin concentrations gut. Further work required for be potential therapy.

Язык: Английский

---

Identification and Functional Analysis of Novel SNPs in Enterocin Genes of Enterococcus faecium GHB21

Probiotics and Antimicrobial Proteins, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Язык: Английский

---

Natural Antimicrobial Compounds in Food Safety

Опубликована: Янв. 1, 2025

Язык: Английский

---

EFFECT OF DIFFERENT CULTURE MEDIA, INITIAL pH, INCUBATION TEMPERATURE, AND CARBON SOURCES ON GROWTH AND BACTERIOCIN PRODUCTION OF ENTEROCOCCUS MUNDTII YB6.30

GIDA / THE JOURNAL OF FOOD, Год журнала: 2025, Номер 50(2), С. 245 - 259

Опубликована: Апрель 7, 2025

This study investigated the effects of various factors on production mundticin-KS in Enterococcus mundtii YB6.30 strain, previously isolated from Sucuk, a dry-fermented sausage. The examined included culture medium (de Man, Rogosa, and Sharpe broth; Brain Heart Infusion M17 Luria-Bertani Trypticase Soy Broth), initial pH (4.5, 5.5, 6.2, 7.4, 8.5), incubation temperature (25°C, 30°C, 37°C, 40°C), carbon source (glucose, fructose, lactose, or sucrose), sucrose concentration (1%, 2%, 3%, 4%, 5%, 7.5%, 10%). A one-factor-at-a-time (OFAT) approach was employed to determine influencing bacteriocin production. Statistical analysis revealed that optimal by E. achieved after 6 8 hours at 30°C modified MRS broth medium, adjusted an 6.2 with addition 1% (w/v) sucrose. activity under these conditions measured 1495.62±29.93 AU/mL 6th hour 1567.21±26.27 8th (P>0.05).

Язык: Английский

---