Current Research in Microbial Sciences, Год журнала: 2024, Номер 7, С. 100296 - 100296
Опубликована: Янв. 1, 2024
Extra-intestinal pathogenic
Язык: Английский
Biology, Год журнала: 2025, Номер 14(2), С. 165 - 165
Опубликована: Фев. 6, 2025
Healthcare-associated infections pose a significant global health challenge, negatively impacting patient outcomes and burdening healthcare systems. A major contributing factor to healthcare-associated is the formation of biofilms, structured microbial communities encased in self-produced extracellular polymeric substance matrix. Biofilms are critical disease etiology antibiotic resistance, complicating treatment infection control efforts. Their inherent resistance mechanisms enable them withstand therapies, leading recurrent increased morbidity. This review explores development biofilms their dual roles disease. It highlights structural protective functions EPS matrix, which shields populations from immune responses antimicrobial agents. Key molecular biofilm including restricted penetration, persister cell dormancy, genetic adaptations, identified as barriers effective management. implicated various clinical contexts, chronic wounds, medical device-associated infections, oral complications, surgical site infections. prevalence hospital environments exacerbates challenges underscores urgent need for innovative preventive therapeutic strategies. evaluates cutting-edge approaches such DNase-mediated disruption, RNAIII-inhibiting peptides, DNABII proteins, bacteriophage nanoparticle-based solutions, coatings, lock therapies. also examines associated with biofilm-related diagnostic difficulties, disinfectant economic implications. emphasizes multidisciplinary approach importance understanding dynamics, role pathogenesis, advancements strategies combat biofilm-associated effectively settings. These insights aim enhance reduce burden diseases.
Язык: Английский
Процитировано
3
Inorganics, Год журнала: 2025, Номер 13(2), С. 65 - 65
Опубликована: Фев. 19, 2025
Proteus mirabilis has been identified as the third most frequent reason for catheter-associated urinary tract infections. The production of urease significantly enhances force catheter blockage caused by biofilm formation. Because biofilms are important virulence factors that make antibiotics less potent, it is becoming increasingly to develop novel alternative antibiotics. In addition unique properties they possess, nanoparticles made from metal oxide currently attracting considerable attention possible antibacterials. This research aims explore potential anti-biofilm green manufactured ZnO-CuO generated P. mirabilis. By synthesizing reductive enzymes, bacterial cells can participate in biosynthesis process. study explores whether synthesized work an agent formed These were using Bacteriocins determine their effectiveness against bacteria, which partially purified and showed antimicrobial activity Gram-negative bacteria AFM, TEM, FESEM, XRD, ultraviolet (UV)–visible spectroscopy used analyze biosynthesized ascertain chemical physical characteristics. XRD verified hexagonal structure, TEM demonstrated a size range 96.00 nm, FESEM surface morphology. dispersion roughness shown through AFM examination. produced nanoparticles’ UV-visible spectra displayed maximum peak at 287 232 nm. When applied strains (wild-type) (multidrug-resistant), copper zinc had notable inhibitory effects. Weak effectively generate biofilms, following incubation with 128 μg/mL subminimum concentrations (MICs) CuO 24 48 h 37 °C. Following treatment nanocomposite these strains, downregulation alterations LuxS expression detected utilizing real-time PCR After this, isolates treated nanocomposite, downregulated shifts found technique contrast not treated. Zinc (ZnO) be utilized antibacterial agents concentration-dependent manner, aligning all observed findings. present demonstrates oxide–zinc nanocomposites effective Their noteworthy gene successfully prevents formation swarming motility.
Язык: Английский
Процитировано
1
OTA International The Open Access Journal of Orthopaedic Trauma, Год журнала: 2025, Номер 8(1S)
Опубликована: Март 1, 2025
Preclinical models of osseointegrated orthopaedic implants tend to focus on implant stability, surface modifications enhance integration with host tissue, and reduction in iatrogenic contamination through antibiotic-eluting/bacteria-resistant coatings. While these studies are imperative early success osseointegration, continued percutaneous devices throughout the lifespan patient is also critically important. A perpetual challenge formation bacterial biofilm abutment. Once adhered, biofilm-based bacteria recalcitrant readily contaminate subcutaneous soft tissue stoma. To this end, rabbit model reported herein replicates clinical scenario a biofilm-contaminated This enables preclinical testing advanced therapeutics beyond traditional antibiotic-based approach, potentially increasing longevity device.
Язык: Английский
Процитировано
0
Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2025, Номер 24(3)
Опубликована: Апрель 22, 2025
Abstract Biofilm formation on dairy equipment is a persistent challenge in the industry, contributing to product contamination, inefficiency, and economic losses. Traditional methods such as manual cleaning basic chemical sanitation are discussed foundational approaches, followed by an in‐depth investigation of cutting‐edge technologies, including clean‐in‐place systems, high‐pressure cleaning, foam ultrasonic electrochemical dry ice blasting, robotics, nanotechnology‐based agents, enzymatic cleaners, oxidizing agents. Enhanced techniques, steam, pulsed light, acidic alkaline electrolyzed water, hydrogen peroxide vapor, microbubble technology, biodegradable biocides, highlighted for their potential achieve superior while promoting sustainability. The effectiveness, feasibility, limitations these evaluated, emphasizing role maintaining hygiene reducing biofilm‐associated risks. Additionally, challenges, compatibility, cost, regulatory compliance, addressed, along with insights into future directions innovations, automation, smart green solutions. This review provides comprehensive resource researchers, industry professionals, policymakers aiming tackle biofilm production systems enhance food safety, operational efficiency,
Язык: Английский
Процитировано
0
Molecular Pharmaceutics, Год журнала: 2025, Номер unknown
Опубликована: Май 27, 2025
Deep skin fungal infections, particularly biofilm-associated Candida albicans (C. albicans), pose significant clinical challenges due to their resistance conventional antifungal therapies. The stratum corneum acts as a barrier high molecular weight drugs, limiting the penetration of systemic and topical agents. In this study, we explored transdermal delivery system utilizing novel Cathelicidin─HcCATH-KL30 (CATH)-loaded dissolving microneedles (DMNs) for treatment deep dermal C. biofilm infections. Preliminary evaluations demonstrated that CATH exhibited potent activity against nonfilamentous filamentous but was ineffective biofilm-embedded Candida, suggesting limitations. To enhance its efficacy, integrated indocyanine green into DMNs applied photodynamic therapy (PDT) using near-infrared (NIR) irradiation. generated reactive oxygen species disrupted matrix, allowing deeper enhanced activity. Results from in vitro, ex vivo, vivo models reduction (∼94%) burden with CATH-ICG-DMNs following NIR irradiation, highlighting synergistic effect. Findings study were mechanistically validated through qRT-PCR propodeum iodide staining, which accordance proposed hypothesis. current research work first time antimicrobial peptide drug platform order investigate potential. This establishes promising microneedle-based PDT strategy combating overcoming biofilm-mediated enhancing efficacy.
Язык: Английский
Процитировано
0
Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107780 - 107780
Опубликована: Июнь 1, 2025
Язык: Английский
Процитировано
0
Current Research in Microbial Sciences, Год журнала: 2024, Номер 7, С. 100296 - 100296
Опубликована: Янв. 1, 2024
Extra-intestinal pathogenic
Язык: Английский
Процитировано
2