Pharmaceutics,
Год журнала:
2022,
Номер
14(8), С. 1529 - 1529
Опубликована: Июль 22, 2022
Pharmaceutical
and
personal
care
industries
require
human
representative
models
for
testing
to
ensure
the
safety
of
their
products.
A
major
route
penetration
into
our
body
after
substance
exposure
is
via
skin.
Our
aim
was
generate
robust
culture
conditions
a
next
generation
skin-on-chip
model
containing
neopapillae
establish
proof-of-concept
with
sensitizer,
cinnamaldehyde.
Reconstructed
skin
consisting
stratified
differentiated
epidermis
on
fibroblast
populated
hydrogel
spheroids
(RhS-NP),
were
cultured
air-exposed
under
dynamic
flow
10
days.
The
robustness
three
independent
experiments,
each
up
21
intra-experiment
replicates,
investigated.
seen
invaginate
towards
neopapille
spheroids.
Daily
measurements
lactate
dehydrogenase
(LDH)
glucose
levels
within
medium
demonstrated
high
viability
stable
metabolic
activity
throughout
period
in
all
experiments
replicates
an
experiment.
Topical
cinnamaldehyde
RhS-NP
resulted
dose-dependent
cytotoxicity
(increased
LDH
release)
elevated
cytokine
secretion
contact
sensitizer
specific
IL-18,
pro-inflammatory
IL-1β,
inflammatory
IL-23
IFN-γ,
as
well
anti-inflammatory
IL-10
IL-12p70.
This
study
demonstrates
feasibility
complex
investigating
immunotoxicity.
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Март 26, 2025
Traditional
toxicological
assessment
relied
heavily
on
2D
cell
cultures
and
animal
models
of
study,
which
were
inadequate
for
the
precise
prediction
human
response
to
chemicals.
Researchers
have
now
shifted
focus
organoids
assessment.
Organoids
are
3D
structures
produced
from
stem
cells
that
mimic
shape
functionality
organs
a
number
advantages
compared
traditional
study.
They
capacity
replicate
intricate
cellular
microenvironment
in
vivo
interactions.
offer
physiologically
pertinent
platform
is
useful
researchers
monitor
responses
more
realistic
manner
evaluate
drug
toxicity.
Additionally,
can
be
created
unique
patient,
allowing
individualized
research
providing
understanding
inter-individual
heterogeneity
responses.
Recent
developments
use
gut
liver
xenobiotics
(environmental
toxins
drugs)
reviewed
this
article.
Gut
reveal
potential
damage
digestive
system
how
affect
nutrient
absorption
barrier
function.
Liver
primary
site
detoxification
metabolism
xenobiotics,
usually
routed
gut.
Hence,
these
linked
crucial
evaluating
chemical
or
pollutant
induced
organ
toxicity,
forecasting
their
pharmacokinetics.
When
incorporated
into
development
process,
organoid
improve
accuracy
efficiency
safety
assessments,
leading
safer
effective
treatments.
We
also
discuss
limitations
using
organoid-based
assays,
future
prospects,
including
need
standardized
protocols
overcoming
reproducibility
issues.
Pharmaceutics,
Год журнала:
2022,
Номер
14(8), С. 1529 - 1529
Опубликована: Июль 22, 2022
Pharmaceutical
and
personal
care
industries
require
human
representative
models
for
testing
to
ensure
the
safety
of
their
products.
A
major
route
penetration
into
our
body
after
substance
exposure
is
via
skin.
Our
aim
was
generate
robust
culture
conditions
a
next
generation
skin-on-chip
model
containing
neopapillae
establish
proof-of-concept
with
sensitizer,
cinnamaldehyde.
Reconstructed
skin
consisting
stratified
differentiated
epidermis
on
fibroblast
populated
hydrogel
spheroids
(RhS-NP),
were
cultured
air-exposed
under
dynamic
flow
10
days.
The
robustness
three
independent
experiments,
each
up
21
intra-experiment
replicates,
investigated.
seen
invaginate
towards
neopapille
spheroids.
Daily
measurements
lactate
dehydrogenase
(LDH)
glucose
levels
within
medium
demonstrated
high
viability
stable
metabolic
activity
throughout
period
in
all
experiments
replicates
an
experiment.
Topical
cinnamaldehyde
RhS-NP
resulted
dose-dependent
cytotoxicity
(increased
LDH
release)
elevated
cytokine
secretion
contact
sensitizer
specific
IL-18,
pro-inflammatory
IL-1β,
inflammatory
IL-23
IFN-γ,
as
well
anti-inflammatory
IL-10
IL-12p70.
This
study
demonstrates
feasibility
complex
investigating
immunotoxicity.