Proof-of-Concept Organ-on-Chip Study: Topical Cinnamaldehyde Exposure of Reconstructed Human Skin with Integrated Neopapillae Cultured under Dynamic Flow DOI Creative Commons

Irit Vahav,

Maria Thon, Lenie J. van den Broek

и другие.

Pharmaceutics, Год журнала: 2022, Номер 14(8), С. 1529 - 1529

Опубликована: Июль 22, 2022

Pharmaceutical and personal care industries require human representative models for testing to ensure the safety of their products. A major route penetration into our body after substance exposure is via skin. Our aim was generate robust culture conditions a next generation skin-on-chip model containing neopapillae establish proof-of-concept with sensitizer, cinnamaldehyde. Reconstructed skin consisting stratified differentiated epidermis on fibroblast populated hydrogel spheroids (RhS-NP), were cultured air-exposed under dynamic flow 10 days. The robustness three independent experiments, each up 21 intra-experiment replicates, investigated. seen invaginate towards neopapille spheroids. Daily measurements lactate dehydrogenase (LDH) glucose levels within medium demonstrated high viability stable metabolic activity throughout period in all experiments replicates an experiment. Topical cinnamaldehyde RhS-NP resulted dose-dependent cytotoxicity (increased LDH release) elevated cytokine secretion contact sensitizer specific IL-18, pro-inflammatory IL-1β, inflammatory IL-23 IFN-γ, as well anti-inflammatory IL-10 IL-12p70. This study demonstrates feasibility complex investigating immunotoxicity.

Язык: Английский

From gut to liver: organoids as platforms for next-generation toxicology assessment vehicles for xenobiotics DOI Creative Commons
Sulaiman Mohammed Alnasser

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Март 26, 2025

Traditional toxicological assessment relied heavily on 2D cell cultures and animal models of study, which were inadequate for the precise prediction human response to chemicals. Researchers have now shifted focus organoids assessment. Organoids are 3D structures produced from stem cells that mimic shape functionality organs a number advantages compared traditional study. They capacity replicate intricate cellular microenvironment in vivo interactions. offer physiologically pertinent platform is useful researchers monitor responses more realistic manner evaluate drug toxicity. Additionally, can be created unique patient, allowing individualized research providing understanding inter-individual heterogeneity responses. Recent developments use gut liver xenobiotics (environmental toxins drugs) reviewed this article. Gut reveal potential damage digestive system how affect nutrient absorption barrier function. Liver primary site detoxification metabolism xenobiotics, usually routed gut. Hence, these linked crucial evaluating chemical or pollutant induced organ toxicity, forecasting their pharmacokinetics. When incorporated into development process, organoid improve accuracy efficiency safety assessments, leading safer effective treatments. We also discuss limitations using organoid-based assays, future prospects, including need standardized protocols overcoming reproducibility issues.

Язык: Английский

Процитировано

0
Innovative approaches to blood-brain barrier modelling: The chip revolution DOI Creative Commons

Avijit Bej,

Nawajes Mandal, Anwesha Das

и другие.

Next Nanotechnology, Год журнала: 2025, Номер 7, С. 100171 - 100171

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Compartmentalized perfusion for temporal control of the chemical microenvironment of iPSC-derived cardiac cells DOI Creative Commons
Kaisa Tornberg, Wolfram Grötsch,

Niina Ritari

и другие.

Lab on a Chip, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Organ-on-chip structure for compartmentalized perfusion.

Язык: Английский

Процитировано

0
Foodborne compounds that alter plasma membrane architecture can modify the response of intestinal cells to shear stress in vitro DOI

Valerie I. C. Rebhahn,

Еndre Kiss, Doris Marko

и другие.

Toxicology and Applied Pharmacology, Год журнала: 2022, Номер 446, С. 116034 - 116034

Опубликована: Апрель 21, 2022

Язык: Английский

Процитировано

14
Proof-of-Concept Organ-on-Chip Study: Topical Cinnamaldehyde Exposure of Reconstructed Human Skin with Integrated Neopapillae Cultured under Dynamic Flow DOI Creative Commons

Irit Vahav,

Maria Thon, Lenie J. van den Broek

и другие.

Pharmaceutics, Год журнала: 2022, Номер 14(8), С. 1529 - 1529

Опубликована: Июль 22, 2022

Pharmaceutical and personal care industries require human representative models for testing to ensure the safety of their products. A major route penetration into our body after substance exposure is via skin. Our aim was generate robust culture conditions a next generation skin-on-chip model containing neopapillae establish proof-of-concept with sensitizer, cinnamaldehyde. Reconstructed skin consisting stratified differentiated epidermis on fibroblast populated hydrogel spheroids (RhS-NP), were cultured air-exposed under dynamic flow 10 days. The robustness three independent experiments, each up 21 intra-experiment replicates, investigated. seen invaginate towards neopapille spheroids. Daily measurements lactate dehydrogenase (LDH) glucose levels within medium demonstrated high viability stable metabolic activity throughout period in all experiments replicates an experiment. Topical cinnamaldehyde RhS-NP resulted dose-dependent cytotoxicity (increased LDH release) elevated cytokine secretion contact sensitizer specific IL-18, pro-inflammatory IL-1β, inflammatory IL-23 IFN-γ, as well anti-inflammatory IL-10 IL-12p70. This study demonstrates feasibility complex investigating immunotoxicity.

Язык: Английский

Процитировано

13