Sappanone A attenuates rheumatoid arthritis via inhibiting PI3K/AKT/NF-κB and JAK2/STAT3 signaling pathways in vivo and in vitro
International Immunopharmacology,
Год журнала:
2024,
Номер
143, С. 113560 - 113560
Опубликована: Ноя. 12, 2024
Язык: Английский
Relaxin‐2 Alleviated Ferroptosis and Inflammation in TNF‐α‐Treated Chondrocytes via Regulating the Nrf2/NF‐κB Signaling Pathway
Peptide Science,
Год журнала:
2025,
Номер
117(2)
Опубликована: Янв. 25, 2025
ABSTRACT
Excessive
inflammatory
responses
and
ferroptosis‐associated
dysfunction
of
chondrocytes
are
significant
pathological
features
osteoarthritis
(OA).
Relaxin‐2,
a
well‐known
hormone
involved
in
metabolic
regulation
across
various
tissues
cells,
has
not
been
extensively
studied
the
context
chondrocyte
OA.
T/C‐28a2
cells
were
stimulated
with
tumor
necrosis
factor
α
(TNF‐α)
(10
ng/mL)
or
without
recombinant
human
relaxin‐2
(rh
relaxin‐2)
(25,
50
nM)
for
48
h.
Various
techniques,
including
2′,7′‐dichlorodihydrofluorescein
diacetate
(DCFH‐DA)
staining,
real‐time
PCR,
western
blot
analysis,
enzyme‐linked
immunosorbent
assay
(ELISA),
luciferase
activity
assays,
employed.
We
discovered
that
exposure
to
TNF‐α
decreased
expression
Relaxin‐2
at
both
mRNA
protein
levels.
Further
investigation
revealed
treatment
rh
mitigated
lipid
peroxidation
by
reducing
levels
reactive
oxygen
species
(ROS),
malondialdehyde
(MDA),
4‐hydroxy‐2‐nonenal
(4‐HNE),
while
increasing
superoxide
dismutase
(SOD)
glutathione
(GSH)
content.
Notably,
restored
iron
metabolism
balance
disrupted
decreasing
Fe
2+
levels,
downregulating
transferrin
receptor
1
(TFR1)
gene
expression,
upregulating
ferritin
expression.
Additionally,
alleviated
ferroptosis
induced
these
peroxidase
4
(GPX4)
acyl‐CoA
synthetase
long‐chain
family
member
(ACSL4)
lactate
dehydrogenase
(LDH)
release.
Rh
also
inhibited
TNF‐α‐induced
pro‐inflammatory
cytokines,
interleukin‐1β
(IL‐1β)
interleukin‐6
(IL‐6).
Interestingly,
attenuated
degeneration
suppressing
matrix
metalloproteinase‐13
(MMP‐13)
preventing
degradation
collagen
type
II
alpha1
chain
(Col2α).
Mechanistically,
effects
found
be
mediated
through
nuclear
erythroid
2‐related
2
(Nrf2)/NF‐κB
signaling
pathway.
Our
findings
suggest
could
potential
therapeutic
strategy
Язык: Английский
Potential role of SIRT1 in cell ferroptosis
Frontiers in Cell and Developmental Biology,
Год журнала:
2025,
Номер
13
Опубликована: Март 5, 2025
Ferroptosis
is
a
novel
form
of
cell
death
that
uniquely
requires
iron
and
characterized
by
accumulation,
the
generation
free
radicals
leading
to
oxidative
stress,
formation
lipid
peroxides,
which
distinguish
it
from
other
forms
death.
The
regulation
ferroptosis
extremely
complex
closely
associated
with
spectrum
diseases.
Sirtuin
1
(SIRT1),
NAD
+
-dependent
histone
deacetylase,
has
emerged
as
pivotal
epigenetic
regulator
potential
regulate
through
wide
array
genes
intricately
metabolism,
homeostasis,
glutathione
biosynthesis,
redox
homeostasis.
This
review
provides
comprehensive
overview
specific
mechanisms
SIRT1
regulates
explores
its
therapeutic
value
in
context
multiple
disease
pathologies,
highlighting
significance
SIRT1-mediated
treatment
strategies.
Язык: Английский
Research advances of Sappanone A in inflammation-related diseases
Frontiers in Medicine,
Год журнала:
2025,
Номер
12
Опубликована: Май 8, 2025
Sappanone
A
(SA),
a
kind
of
homoisoflavanone
extracted
from
the
dry
heartwood
Caesalpinia
sappan
L.,
has
been
shown
to
possess
diverse
bioactivities
involving
anti-inflammatory,
antioxidant,
and
anti-apoptotic
properties.
Sustained
proinflammatory
state
is
major
factor
in
occurrence
development
various
diseases.
Given
characteristics
SA,
many
studies
have
explored
effect
SA
on
inflammation-related
diseases,
which
uncovered
multifaceted
therapeutic
potential
such
In
this
mini-review,
we
summarized
current
achievements
diseases
(such
as
myocardial
ischemia-reperfusion
injury,
liver
respiratory
kidney
etc.),
order
provide
useful
insights
into
role
benefit
future
clinical
applications.
Язык: Английский
Mechanisms and Therapeutic Potential of GPX4 in Pain Modulation
Pain and Therapy,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 6, 2024
Pain,
a
complex
symptom
encompassing
both
sensory
and
emotional
dimensions,
constitutes
significant
global
public
health
issue.
Oxidative
stress
is
pivotal
factor
in
the
pathophysiology
of
pain,
with
glutathione
peroxidase
4
(GPX4)
recognized
as
crucial
antioxidant
enzyme
involved
defense
mechanisms
ferroptosis
pathways.
This
review
systematically
explores
GPX4's
functions
across
various
pain
models,
including
neuropathic,
inflammatory,
low
back,
cancer-related
pain.
Specifically,
focus
includes
physiological
roles,
mechanisms,
regulation
ferroptosis,
involvement
signal
transduction
pathways,
metabolic
regulation.
By
summarizing
current
research,
we
highlight
potential
GPX4-targeted
therapies
management.
Язык: Английский
BCL6 Promotes Transcription of GPR61 to Suppress IL‐1β‐Induced Osteoarthritis Progression in C28/I2 Cells
Microbiology and Immunology,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 23, 2024
Abstract
Osteoarthritis
(OA)
is
the
most
common
joint
disease
and
its
pathogenic
mechanism
remains
to
be
ensured.
This
study
focused
on
regulatory
relation
between
B‐cell
lymphoma
6
(BCL6)
G‐protein‐coupled
receptor
61
(GPR61)
underlying
IL‐1β
in
OA.
Real‐time
quantitative
polymerase
chain
reaction
western
blot
were
performed
for
mRNA
protein
detection.
Oxidative
injury
was
assessed
by
reactive
oxygen
species
(ROS),
malondialdehyde
(MDA),
glutathione
(GSH)
via
kits.
Fe
2+
level
measured
an
iron
assay
kit.
Relation
analysis
BCL6
GPR61
implemented
employing
ChIP
dual‐luciferase
reporter
assay.
downregulated
OA
samples
IL‐1β‐induced
C28/I2
cells.
cell
inflammation,
extracellular
matrix
(ECM)
degradation,
oxidative
stress,
ferroptosis
all
returned
overexpression
of
GPR61.
downregulation
detected
patients
IL‐1β‐exposed
could
promote
transcription
suppressed
progression
upregulating
The
BCL6/GPR61
axis
activated
PKA/CREB
pathway
IL‐1β‐treated
above
results
suggested
that
mitigated
induced
enhancing
BCL6/GPR61/PKA/CREB
may
considered
as
a
novel
OA,
has
potential
act
target
Язык: Английский