Bioengineering, Год журнала: 2025, Номер 12(3), С. 273 - 273

Опубликована: Март 10, 2025

This study evaluates four compression testing methods to determine the most reliable and reproducible technique for assessing strength of murine lumbar vertebral bodies. Twenty female C57BL/6 mice (12 weeks old) were randomized into groups: Group 1, complete body (LVB) with dorsal spinal processes; 2, at surface; 3, surface after arch resection; 4, resection straightening intervertebral joint surface. A mono-axial static machine applied compression, measuring load failure, stiffness, yield load, elasticity modulus. Method 1 resulted in significantly higher load-to-failure yield-to-failure (25.9 N compared 18.2 N, twice 12 Methods 2–4), least variation relative values. 3 had increased stiffness a Young’s modulus (232 N/mm, contrast 101, 130, 145 N/mm respectively) but yielded inconsistent results. 4 showed greatest variability across specimens. 2 yields suitable data quality as well, albeit slightly variation, is recommended procedure if processes have be excluded from measurement. Based on these findings, produced consistent future studies evaluating biomechanics mice.

Язык: Английский