ALYREF Promotes Progression of Intrahepatic Cholangiocarcinoma through Increasing the Level of Isocitrate Dehydrogenase 1 in an m5C-Dependent Manner DOI Creative Commons

Zhiqiang Hao,

Haixiang Yang, Wei Zhu

и другие.

Molecular and Cellular Biology, Год журнала: 2025, Номер unknown, С. 1 - 14

Опубликована: Май 14, 2025

RNA 5-methylcytosine (m5C) modification has emerged as an important regulatory mechanism in the progression of human cancers, including hepatobiliary tumors. The m5C "reader" Aly/REF export factor (ALYREF) was recently found to be identified a prognostic biomarker liver cancer. However, its exact role intrahepatic cholangiocarcinoma (ICC) is unclear. In this study, ALYREF upregulated ICC tissues and cells. gain- loss-of-function experiments indicated that promoted cell proliferation invasion suppressed apoptosis. Moreover, we isocitrate dehydrogenase 1 (IDH1), metastatic marker cancer, also tissues, displayed relatively strong positive correlation with level ALYREF, positively regulated by ALYREF. As "reader", interacted m5C-IDH1 mRNA increased stability. knockdown partially eliminated promotion IDH1 on invasion. NRF2-driven glutathione synthesis cells, which reversed silencing. Finally, xenograft tumor mouse model, or treatment ivosidenib (an inhibitor) significantly growth vivo. conclusion, promotes increasing levels m5C-dependent manner.

Язык: Английский

The m7G methylation modification: An emerging player of cardiovascular diseases DOI

Can Xu,

Xiao-Hua Yu, Gang Wang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 309, С. 142940 - 142940

Опубликована: Апрель 8, 2025

Язык: Английский

Процитировано

0
ALYREF Promotes Progression of Intrahepatic Cholangiocarcinoma through Increasing the Level of Isocitrate Dehydrogenase 1 in an m5C-Dependent Manner DOI Creative Commons

Zhiqiang Hao,

Haixiang Yang, Wei Zhu

и другие.

Molecular and Cellular Biology, Год журнала: 2025, Номер unknown, С. 1 - 14

Опубликована: Май 14, 2025

RNA 5-methylcytosine (m5C) modification has emerged as an important regulatory mechanism in the progression of human cancers, including hepatobiliary tumors. The m5C "reader" Aly/REF export factor (ALYREF) was recently found to be identified a prognostic biomarker liver cancer. However, its exact role intrahepatic cholangiocarcinoma (ICC) is unclear. In this study, ALYREF upregulated ICC tissues and cells. gain- loss-of-function experiments indicated that promoted cell proliferation invasion suppressed apoptosis. Moreover, we isocitrate dehydrogenase 1 (IDH1), metastatic marker cancer, also tissues, displayed relatively strong positive correlation with level ALYREF, positively regulated by ALYREF. As "reader", interacted m5C-IDH1 mRNA increased stability. knockdown partially eliminated promotion IDH1 on invasion. NRF2-driven glutathione synthesis cells, which reversed silencing. Finally, xenograft tumor mouse model, or treatment ivosidenib (an inhibitor) significantly growth vivo. conclusion, promotes increasing levels m5C-dependent manner.

Язык: Английский

Процитировано

0