Hepatoprotective Effects of Royal Jelly Against Vincristine-Induced Hepatotoxicity in Rats: A Biochemical and Molecular Study
Life,
Год журнала:
2025,
Номер
15(3), С. 459 - 459
Опубликована: Март 14, 2025
Vincristine
(VCR)
is
a
chemotherapeutic
agent
classified
as
vinca
alkaloid.
Royal
jelly
(RJ)
significant
bee
product
produced
by
worker
bees,
characterized
its
high
protein
content.
This
study
aims
to
investigate
the
protective
effects
of
RJ
against
VCR-induced
liver
damage.
VCR
was
intraperitoneally
administered
at
dose
0.1
mg/kg
body
weight
(b.w.)
and
orally
doses
150
300
b.w.
Both
treatments
were
applied
rats
on
days
1–6
9–14.
The
composition
analyzed
using
LC-MS/MS,
revealing
presence
15
different
phytochemical
compounds
with
strong
antioxidant
properties.
Serum
samples
obtained
from
for
ALT,
ALP,
AST
levels.
While
these
enzyme
levels
significantly
elevated
in
group,
notable
reduction
observed
following
administration.
Additionally,
SOD,
CAT,
GPx,
GSH
parameters,
along
MDA
levels,
evaluated
tissue
samples.
results
indicated
decrease
activities/levels
an
increase
group.
Furthermore,
ELISA
used
assess
JAK2,
STAT3,
mTOR/PI3K/AKT
signaling
pathways.
administration
led
JAK2
STAT3
In
addition,
mRNA
transcription
inflammation
(NF-κB,
TNF-α,
IL-1β),
endoplasmic
reticulum
(ER)
stress
(IRE-1,
GRP78,
PERK,
ATF-6),
autophagy
markers
(LC3A
LC3B)
examined.
A
inflammation,
ER
stress,
autophagy-related
VCR-treated
Lastly,
expression
Bax,
Bcl-2,
Caspase-3,
NF-κB
evaluated.
treatment
increased
Caspase
3,
whereas
Bcl-2
decreased.
However,
administration,
all
parameters
reversed,
demonstrating
improvements.
conclusion,
findings
suggest
that
may
exert
effect
Язык: Английский
Green Extraction Strategies and Bioactivity of Rheum cordatum Losinsk: Antioxidant, Antimicrobial, and Molecular Docking Insights
Plants,
Год журнала:
2025,
Номер
14(7), С. 1071 - 1071
Опубликована: Апрель 1, 2025
This
study
aimed
to
compare
the
efficiency
of
different
green
extraction
methods
for
obtaining
bioactive
compounds
from
roots
Rheum
cordatum
Losinsk
and
evaluate
their
antioxidant
antimicrobial
properties.
The
presence
some
important
phytochemicals
in
extracts
obtained
using
ultrasound-assisted
(UAE),
subcritical
ethanol
(Sbc-EtOH),
supercritical
CO2
(ScCO2)
was
determined
by
LC-MS/MS,
properties
were
examined
against
Staphylococcus
aureus,
Enterococcus
faecalis,
Pseudomonas
aeruginosa,
Escherichia
coli.
goal
determine
optimal
conditions
that
maximize
yield
while
preserving
biological
Different
pressures
(100
bar
400
bar)
tested
UAE
extraction,
solvents
times
Sbc-EtOH
ScCO2
extraction.
Most
53
phenolic
have
been
extracted
method,
either
exclusively
or
highest
amounts.
It
has
observed
more
higher
amounts
at
lower
pressure.
activity
exhibited
extracts.
Additionally,
ScCO2-100
extract
100
showed
strong
activity,
with
a
minimum
inhibitory
concentration
(MIC)
ranging
31.25
250
μg/mL.
Gallic
acid,
epicatechin
gallate,
epigallocatechin
catechin
found
molecular
docking
studies
1QWZ,
2ANQ,
3H77,
6QXS
proteins
revealed
scores
-6.127,
-9.479,
-5.836,
-7.067
kcal/mol,
respectively.
Язык: Английский
Hepatoprotective Activity of Prunus laurocerasus Fruit Extract Against 5‐FU‐Induced Liver Injury and LC‐MS/MS Profiling
Chemistry & Biodiversity,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 30, 2025
ABSTRACT
5‐Fluorouracil
(5‐FU),
one
of
the
most
widely
used
chemotherapy
drugs,
is
known
to
cause
widespread
toxicity
and
irreparable
tissue
damage.
The
purpose
this
work
was
analyze
phytochemical
components
Prunus
laurocerasus
(PL)
fruit
extract
using
liquid
chromatography
with
tandem
mass
spectrometry
(LC‐MS/MS)
look
into
effectiveness
PL
pretreatment
on
5‐FU‐induced
liver
study
consisted
control
(C),
PL,
5‐FU,
PL+5‐FU
groups,
groups
received
500
mg/kg
as
for
seven
days.
On
eighth
day,
100
5‐FU
administered
intraperitoneally
a
single
dose
groups.
In
LC‐MS/MS
results,
it
determined
that
phenolic
compounds
found
in
foreground
were
quinic
acid
chlorogenic
acid.
increased
total
antioxidant
status
levels
B‐cell
lymphoma
2
(Bcl‐2)
expression
also
decreased
Keap1,
oxidant
status,
oxidative
stress
index
levels,
Bcl‐2‐associated
X
protein,
Caspase‐3,
light
chain‐3B
expressions.
8‐hydroxy‐2'‐deoxyguanosine
higher
group
compared
C
Moreover,
group,
similar
C,
conclusion,
demonstrated
hepatoprotective
action
via
regulating
Kelch‐like
ECH‐associated
protein
1/nuclear
factor‐erythroid
2‐related
factor
pathway
damage,
lowering
stress,
decreasing
apoptosis
autophagy
from
programmed
cell
death.
Язык: Английский