Journal of Biochemical and Molecular Toxicology, Год журнала: 2025, Номер 39(5)
Опубликована: Май 1, 2025
ABSTRACT Methotrexate (MTX) has been widely implemented in managing several malignancies, inflammatory conditions such as rheumatic arthritis, and autoimmune illnesses. Hepatotoxicity is a significant side effect of MTX, characterized by increased oxidative stress (OS) inflammation. Vinpocetine (Vinpo) prescription medication with favorable safety profile. It exerts anti‐inflammatory oxidant implications that might be novel candidates for protecting against MTX‐induced hepatotoxicity. This study investigates the therapeutic impact Vinpo MTX‐stimulated liver damage via nuclear factor erythroid 2‐related 2 (Nrf2)/heme oxygenase‐1 (HO‐1) kappa‐light‐chain‐enhancer activated B cells (NF‐κB) pathways. Rats are allocated into three groups: (1) Control (saline); (2) MTX‐control (20 mg/kg; injected once i.p.), (3) + MTX groups. was administered orally 7 days, during which given intraperitoneally at end Day 3. The functions, OS markers, mediators, Nrf2, HO‐1, NF‐κB, apoptotic signals were estimated. lead to enhancement superoxide dismutase (SOD) enzyme activity, elevation glutathione (GSH), hindrance malondialdehyde (MDA). also enhances Nrf2 inhibiting NF‐κB (p65) expression markers. Moreover, therapy, conjunction restores normal histological structure hepatic tissues. Our data suggested preventive toxicity through anti‐oxidative, anti‐inflammatory, activities, mediated Nrf2/HO‐1/Nf‐κB caspase‐3/Bax/Bcl‐2
Язык: Английский