Tacrolimus
(FK506)
is
a
macrolide
lactone
immunosuppressive
drug
that
commonly
used
in
transplanted
patients
to
avoid
organ
rejection.
FK506
exhibits
high
inter-
and
intra-patient
pharmacokinetic
variability,
making
monitoring
necessary
for
graft
survival.
This
work
describes
the
development
of
novel
bioassay
FK506.
The
based
on
using
magnetic
Janus
micromotors
made
from
polycaprolactone
(PCL)
recombinant
chimera
receptor
incorporates
immunophilin
tacrolimus
binding
protein
1A
(FKBP1A)
tagged
with
Emerald
Green
Fluorescent
Protein
(EmGFP).
approach
relies
fluorescence
competitive
between
decorated
carboxylated
toward
specific
site
fluorescent
immunophilin.
proposed
homogeneous
assay
could
be
performed
single
step
without
wash
steps
separate
unbound
receptor.
fits
therapeutic
requirements,
showing
limit
detection
0.8
ng/mL
wide
dynamic
range
up
90
ng/mL.
Assay
selectivity
was
evaluated
by
measuring
inhibition
curves
other
drugs
usually
co-administered
propulsion
mechanism
allows
efficient
operation
raw
samples
damaging
biological
(FKBP1A-EmGFP).
enhanced
target
recognition
micromixing
strategies
hold
considerable
potential
practical
clinical
use.
Abstract
Ensuring
food
quality
and
safety
according
to
stringent
global
standards
requires
analytical
procedures
that
are
accurate,
cost‐effective,
efficient.
This
present
innovative
high‐throughput
microrobots
designed
for
the
detection
of
antioxidants
in
samples.
These
consist
photocatalytic
bismuth
subcarbonate
anchored
on
silica‐coated
magnetite
nanoparticles.
Upon
exposure
UV
light,
they
generate
reactive
oxygen
species
via
photocatalysis,
which
oxidize
colorless
dye
into
a
green‐colored
radical
cation.
The
presence
inhibits
this
reaction,
allowing
quantification
antioxidant
activity.
magnetic
Fe₃O₄/SiO₂
core
enables
steering
using
transverse
rotating
field,
facilitating
automated
assays
custom‐designed
3D‐printed
sensing
platform.
results
demonstrate
these
magneto‐photocatalytic
can
perform
automated,
assessments
quality,
representing
significant
advancement
analysis
technology.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 21, 2025
Anisotropic
microcarriers
(AMs)
have
attracted
increasing
attention.
Although
significant
efforts
been
made
to
explore
AMs
with
various
morphologies,
their
full
potential
is
yet
be
realized,
as
most
studies
primarily
focused
on
materials
or
fabrication
methods.
A
thorough
analysis
of
the
interactional
and
interdependent
relationships
between
these
factors
required,
along
proposed
countermeasures
tailored
for
researchers
from
backgrounds.
These
include
specific
strategies
morphologies
guidelines
selecting
suitable
AM
certain
biomedical
applications.
In
this
review,
a
comprehensive
summary
AMs,
ranging
methods
applications,
based
past
two
decades
research,
provided.
The
investigated
using
different
corresponding
By
systematically
examining
morphology-dependent
effects,
better
utilization
diverse
can
achieved
clear
breakthroughs
in
field
are
established.
Additionally,
challenges
identified,
new
frontiers
opened,
promising
exciting
opportunities
provided
fabricating
functional
broad
implications
across
fields
that
must
addressed
biomaterials
biotechnology.
Advanced Materials Technologies,
Год журнала:
2024,
Номер
unknown
Опубликована: Май 16, 2024
Abstract
Biomimetic
and
biohybrid
micro/nano‐structures
involve
the
replication
creation
of
technologies,
structures,
materials
based
on
biological
systems
at
micrometer
nanometer
scale.
These
strategies
harness
natural
principles
to
develop
innovative
treatment
methods
advanced
microstructure
devices
for
noninvasive
therapies.
In
this
study,
a
detailed
overview
fabrication
processes,
magnetically
assisted
locomotive
techniques,
potential
applications
biomimetic
micro/nano‐machines
are
presented.
The
latest
advancements
in
actuated
such
as
annelid‐worm‐like
microswimmers,
jellyfish‐shaped
microparticles,
fish‐shaped
walnut‐shaped
micromotors
explored.
Additionally,
magnetic
systems,
including
sunflower
seed‐based
micro‐perforators,
nanomotors
extracted
from
bamboo
stem,
sperm
cell‐based
micromotors,
bacteria‐based
robots,
scaffold‐based
microrobots,
DNA‐based
microalgae‐based
red
blood
microswimmers
also
examined.
A
thorough
investigation
behavior
these
Newtonian
fluids,
featuring
cork‐screw
motion,
undulatory
surface
wrinkling
traveling
wave‐like
ciliary
stroke
motion
is
discussed.
Furthermore,
unprecedented
developed
using
minuscule
cervical
cancer
tetrapod
hybrid
tissue
regeneration
silk
fibroin
protein‐based
microscale
scaffolds,
doxorubicin
drug
delivery
mushroom‐based
microrobots
extensively
Biosensors and Bioelectronics,
Год журнала:
2023,
Номер
244, С. 115796 - 115796
Опубликована: Окт. 27, 2023
Tacrolimus
(FK506)
is
a
macrolide
lactone
immunosuppressive
drug
that
commonly
used
in
transplanted
patients
to
avoid
organ
rejection.
FK506
exhibits
high
inter-
and
intra-patient
pharmacokinetic
variability,
making
monitoring
necessary
for
graft
survival.
This
work
describes
the
development
of
novel
bioassay
FK506.
The
based
on
using
polycaprolactone-based
(PCL)
magnetic
Janus
micromotors
recombinant
chimera
receptor
incorporates
immunophilin
tacrolimus
binding
protein
1A
(FKBP1A)
tagged
with
Emerald
Green
Fluorescent
Protein
(EmGFP).
approach
relies
fluorescence
competitive
between
decorated
carboxylated
toward
specific
site
fluorescent
immunophilin.
proposed
homogeneous
assay
could
be
performed
single
step
without
washing
steps
separate
unbound
receptor.
fits
therapeutic
requirements,
showing
limit
detection
0.8
ng/mL
wide
dynamic
range
up
90
ng/mL.
Assay
selectivity
was
evaluated
by
measuring
inhibition
curves
other
drugs
usually
co-administered
propulsion
mechanism
allows
efficient
operation
raw
samples
damaging
biological
(FKBP1A-EmGFP).
enhanced
target
recognition
micromixing
strategies
hold
considerable
potential
practical
clinical
use.
Journal of Materials Chemistry B,
Год журнала:
2023,
Номер
11(37), С. 8897 - 8915
Опубликована: Янв. 1, 2023
In
the
microscopic
world,
synthetic
micro/nanomotors
(MNMs)
can
convert
a
variety
of
energy
sources
into
driving
forces
to
help
humans
perform
number
complex
tasks
with
greater
ease
and
efficiency.
These
tiny
machines
have
attracted
tremendous
attention
in
field
drug
delivery,
minimally
invasive
surgery,
vivo
sampling,
environmental
management.
By
modifying
their
surface
materials
functionalizing
them
bioactive
agents,
these
MNMs
also
be
transformed
dynamic
micro/nano-biosensors
that
detect
biomolecules
real-time
high
sensitivity.
The
extensive
range
operations
uses
combined
minuscule
size
opened
up
new
avenues
for
tackling
intricate
analytical
difficulties.
Here,
this
review,
various
methods
are
briefly
introduced,
followed
by
focus
on
intelligent
detection
techniques
based
MNMs.
And
we
discuss
distinctive
advantages,
current
issues,
challenges
associated
MNM-based
detection.
It
is
believed
future
advancements
will
greatly
impact
diagnosis,
treatment,
prevention
diseases.
Nanoscale,
Год журнала:
2023,
Номер
15(32), С. 13172 - 13186
Опубликована: Янв. 1, 2023
We
elucidate
the
advancement
of
micro/nanomotor-based
nucleic
acid
biosensing
over
past
decade,
including
fluorescence
recovery-based
biosensing,
velocity
change-based
and
aggregation-enhanced
biosensing.
Angewandte Chemie,
Год журнала:
2024,
Номер
136(16)
Опубликована: Фев. 24, 2024
Abstract
Self‐propelled
micro/nanomotors
(MNMs)
have
shown
great
application
potential
in
biomedicine,
sensing,
environmental
remediation,
etc.
In
the
past
decade,
various
strategies
or
technologies
been
used
to
prepare
and
functionalize
MNMs.
However,
current
preparation
of
MNMs
were
mainly
following
pre‐designed
methods
based
on
specific
tasks
introduce
expected
functional
parts
micro/nanocarriers,
which
lacks
a
universal
platform
common
features,
making
it
difficult
apply
different
scenarios.
Here,
we
developed
modular
assembly
strategy
host–guest
chemistry,
enables
on‐demand
construction
imaging‐trackable
nanomotors
mounted
with
suitable
driving
imaging
modules
using
platform,
according
These
assembled
exhibited
enhanced
diffusion
behavior
driven
by
enzymatic
reactions.
The
loaded
functions
dynamically
trace
swarm
motion
corresponding
fuel
conditions
both
vitro
vivo.
endowed
interaction
provides
approach
producing
multifunctional
facile
controllable
manner,
paves
way
for
future
development
systems
programmable
functions.
