Recent advances in electrochemiluminescence sensing for in vitro cell analysis: a review
Analytical Sciences,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 7, 2025
Electrochemiluminescence
(ECL)
is
a
chemiluminescence
phenomenon
triggered
by
electrochemical
reactions
and
widely
used
for
(bio)chemical
analyses
bioimaging.
Compared
to
fluorescence
sensing,
ECL
sensing
reduces
background
noise
eliminating
autofluorescence
associated
with
excitation
light.
In
addition,
compared
conventional
imaging
scanning
microscopes,
faster
as
it
requires
no
scanning.
Furthermore,
unlike
electrode
arrays,
devices
can
function
without
complex
wiring,
simplifying
their
construction.
These
characteristics
render
useful
analytical
tool.
Recently,
has
been
in
vitro
cell
analysis
due
high
demand
biochips
regenerative
medicine,
drug
screening,
microphysiological
systems.
This
review
focuses
on
recent
advancements
ECL-based
applications
the
detection
of
H2O2,
respiration
activity,
adhesion,
lipid
membranes,
bipolar
electrochemistry-based
devices.
Язык: Английский
Electrochemiluminescence Microscopy of Diffusive Biocompounds as Co-Reactants in Cell Spheroids with [Ru(Bpy)3]2+
Tomas Mockaitis,
Ryota Shikuwa,
Kimiharu Oba
и другие.
Опубликована: Янв. 1, 2025
Язык: Английский
Electrochemiluminescence Microscopy of Diffusive Biocompounds as Co-Reactants in Cell Spheroids with [Ru(bpy)3]2+
Tomas Mockaitis,
Ryota Shikuwa,
Yoshinobu Utagawa
и другие.
Sensors and Actuators B Chemical,
Год журнала:
2025,
Номер
unknown, С. 137944 - 137944
Опубликована: Май 1, 2025
Язык: Английский
Multiphase Electrochemiluminescence of Microdroplets and Radical Salts
Accounts of Chemical Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 3, 2025
ConspectusOver
the
past
decade,
experiments
involving
microdroplets
have
challenged
framework
of
chemistry.
These
droplets
constitute
a
multiphase
system,
where
dynamic
interplay
between
solid,
liquid,
and
gas
influences
reactions.
Multiphase
systems
are
prevalent
in
nature
possess
unique
physicochemical
properties.
However,
chemistry,
phase
boundary
reactivity
is
often
overlooked
because
molecules
experience
"bulk"
reactivity.
biological
processes,
such
as
cell
mitosis,
sensor
technology,
organic
synthesis,
pollution
remediation.Recently,
our
group
has
developed
different
strategies
electrochemiluminescence
(also
called
electrogenerated
chemiluminescence,
both
shortened
to
ECL)
microscopy
imaging
understand
properties
dynamics
at
electrified
interfaces.
ECL
takes
advantage
luminophore
that
radically
annihilates
with
strong
oxidizing
or
reducing
reagent.
If
enthalpy
annihilation
high
enough,
will
be
left
an
excited
state
radiatively
decay,
producing
light.
Thus,
requires
no
incident
light,
analytical
figures
merit
due
light
being
emitted
close
interface
(1-10
μm),
providing
insight
into
within
electrode's
proximity.This
Account
detail
group's
efforts
discovering
reactions
environments
exhibiting
native
triphasic
(liquid|liquid|electrode)
interfaces
new
phases
formed
(e.g.,
bubble
nucleation
electroprecipitation).
We
first
began
developing
tools
necessary
image
liquid|liquid
discovered
that,
when
neighboring
fuse
together,
small
pockets
(inclusions)
continuous
existed
inside
merged
droplets.
Studies
inclusion
chemical
led
interesting
observation
on
can
act
micropumps,
protecting
electrode
from
buildup
during
electrocatalytic
reactions.Even
though
strength
emission
confined
directly
surface,
this
also
significant
weakness,
considering
phenomena
occur
far
away
surface.
One
recent
thrust
community
ways
using
phenomenon
termed
"Through-Space
ECL".
Our
used
technique
measure
forces
boundaries
surface.By
playing
relative
solubilities
reactants
products,
we
showed
if
radical
ion
generated
precipitates
more
quickly
than
its
lifetime,
salts
formed.
way
fossilize
highly
energetic
molecules.
seemingly
tenet
effectively
bottle
up
ECL,
fossilizing
elsewhere
space
time.
Given
passion
teach
world
beauties
electrochemistry,
concepts
surrounding
pedagogically
innovate
performed
by
children
visualize
competing
reactions.In
depict
current
spectroscopy
focus
contributions
burgeoning
field.
field
been
discuss
probability
impact
across
sciences
moving
forward.
Язык: Английский
Shadow electrochemiluminescence imaging of giant liposomes opening at polarized electrodes
The Analyst,
Год журнала:
2024,
Номер
149(12), С. 3317 - 3324
Опубликована: Янв. 1, 2024
In
this
work,
the
release
of
giant
liposome
(∼100
μm
in
diameter)
content
was
imaged
by
shadow
electrochemiluminescence
(ECL)
microscopy.
Giant
unilamellar
liposomes
were
pre-loaded
with
a
sucrose
solution
and
allowed
to
sediment
at
an
ITO
electrode
surface
immersed
containing
luminophore
([Ru(bpy)
Язык: Английский