Recent advances in electrochemiluminescence sensing for in vitro cell analysis: a review

Analytical Sciences, Год журнала: 2025, Номер unknown

Опубликована: Фев. 7, 2025

Electrochemiluminescence (ECL) is a chemiluminescence phenomenon triggered by electrochemical reactions and widely used for (bio)chemical analyses bioimaging. Compared to fluorescence sensing, ECL sensing reduces background noise eliminating autofluorescence associated with excitation light. In addition, compared conventional imaging scanning microscopes, faster as it requires no scanning. Furthermore, unlike electrode arrays, devices can function without complex wiring, simplifying their construction. These characteristics render useful analytical tool. Recently, has been in vitro cell analysis due high demand biochips regenerative medicine, drug screening, microphysiological systems. This review focuses on recent advancements ECL-based applications the detection of H2O2, respiration activity, adhesion, lipid membranes, bipolar electrochemistry-based devices.

Язык: Английский

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Electrochemiluminescence Microscopy of Diffusive Biocompounds as Co-Reactants in Cell Spheroids with [Ru(Bpy)3]2+

Опубликована: Янв. 1, 2025

Язык: Английский

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Electrochemiluminescence Microscopy of Diffusive Biocompounds as Co-Reactants in Cell Spheroids with [Ru(bpy)3]2+

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137944 - 137944

Опубликована: Май 1, 2025

Язык: Английский

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Multiphase Electrochemiluminescence of Microdroplets and Radical Salts

Accounts of Chemical Research, Год журнала: 2025, Номер unknown

Опубликована: Июнь 3, 2025

ConspectusOver the past decade, experiments involving microdroplets have challenged framework of chemistry. These droplets constitute a multiphase system, where dynamic interplay between solid, liquid, and gas influences reactions. Multiphase systems are prevalent in nature possess unique physicochemical properties. However, chemistry, phase boundary reactivity is often overlooked because molecules experience "bulk" reactivity. biological processes, such as cell mitosis, sensor technology, organic synthesis, pollution remediation.Recently, our group has developed different strategies electrochemiluminescence (also called electrogenerated chemiluminescence, both shortened to ECL) microscopy imaging understand properties dynamics at electrified interfaces. ECL takes advantage luminophore that radically annihilates with strong oxidizing or reducing reagent. If enthalpy annihilation high enough, will be left an excited state radiatively decay, producing light. Thus, requires no incident light, analytical figures merit due light being emitted close interface (1-10 μm), providing insight into within electrode's proximity.This Account detail group's efforts discovering reactions environments exhibiting native triphasic (liquid|liquid|electrode) interfaces new phases formed (e.g., bubble nucleation electroprecipitation). We first began developing tools necessary image liquid|liquid discovered that, when neighboring fuse together, small pockets (inclusions) continuous existed inside merged droplets. Studies inclusion chemical led interesting observation on can act micropumps, protecting electrode from buildup during electrocatalytic reactions.Even though strength emission confined directly surface, this also significant weakness, considering phenomena occur far away surface. One recent thrust community ways using phenomenon termed "Through-Space ECL". Our used technique measure forces boundaries surface.By playing relative solubilities reactants products, we showed if radical ion generated precipitates more quickly than its lifetime, salts formed. way fossilize highly energetic molecules. seemingly tenet effectively bottle up ECL, fossilizing elsewhere space time. Given passion teach world beauties electrochemistry, concepts surrounding pedagogically innovate performed by children visualize competing reactions.In depict current spectroscopy focus contributions burgeoning field. field been discuss probability impact across sciences moving forward.

Язык: Английский

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Shadow electrochemiluminescence imaging of giant liposomes opening at polarized electrodes

The Analyst, Год журнала: 2024, Номер 149(12), С. 3317 - 3324

Опубликована: Янв. 1, 2024

In this work, the release of giant liposome (∼100 μm in diameter) content was imaged by shadow electrochemiluminescence (ECL) microscopy. Giant unilamellar liposomes were pre-loaded with a sucrose solution and allowed to sediment at an ITO electrode surface immersed containing luminophore ([Ru(bpy)

Язык: Английский

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