Roadmap for the integration of environmental microbiomes in risk assessments under EFSA's remit
EFSA Supporting Publications,
Год журнала:
2024,
Номер
21(2)
Опубликована: Фев. 1, 2024
Scientific
interest
in
the
use
of
environmental
microbiomes
for
risk
assessment
is
rapidly
growing,
as
exemplified
by
various
EFSA
opinions.
In
absence
official
regulatory
guidelines
on
how
to
integrate
assessment,
aims
this
report
are
therefore
determine
whether
microbiome
studies
can
be
used
such
purposes,
and
propose
a
roadmap
integration
assessments
under
EFSA's
remit.
The
identifies
current
gaps
(in
terms
knowledge
from
technical
point
view)
barriers
that
might
delay
implementation
methods,
offers
recommendations
standardised
(multi-)omics
techniques
purposes.
Our
main
findings
identified
five
priorities:
(i)
defining
core
(what
it
encompasses
what
made
of,
including
identification
bioindicators)
assess
impact
any
type
disturbance;
(ii)
standardising
methodologies
protocols,
sampling
interpretation,
guarantee
comparability
analyses;
(iii)
developing
tools
facilitate
interpretation;
(iv)
collecting
microbiome-based
data
shared,
curated
maintained
databases;
(v)
setting
up
European
Network
Microbiome
Laboratories
reach
an
agreement
standardise
studies,
interactions
between
researchers
access
or
samples,
actively
include
multiple
stakeholders
discussions
involving
assessment.
There
both
short-
longer-term
priorities,
all
which
highlight
need
mobilise
concurrently
different
agencies
institutions,
well
research.
also
points
out
capacity
building
training,
acceptance
emerging
technology,
communication
issues.
These
will
hopefully
contribute
elaboration
widely
accepted
framework
dealing
with.
Язык: Английский
Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests
Frontiers in Microbiology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 8, 2025
The
global
decline
in
biodiversity
and
insect
populations
highlights
the
urgent
need
to
conserve
ecosystem
functions,
such
as
plant
pollination
by
solitary
bees.
Human
activities,
particularly
agricultural
intensification,
pose
significant
threats
these
essential
services.
Changes
land
use
alter
resource
nest
site
availability,
pesticide
exposure
other
factors
impacting
richness,
diversity,
health
of
bee
species.
In
this
study,
we
investigated
yet
another
facet
currently
less
well
context:
Microbial
communities
associated
with
wild
bees
play
crucial
roles
larval
development,
metabolism,
immunity
overall
health.
However,
drivers
dynamics
healthy
microbiome
are
still
poorly
understood,
especially
regarding
direct
indirect
effects
on
diversity
composition
microbial
communities.
We
examined
bacterial
offspring
materials
Megachilid
trap-nesting
bee,
Osmia
bicornis,
along
a
gradient
intensification
16S
rRNA
gene
metabarcoding.
Given
that
landscape
composition,
climatic
conditions,
food
resources
known
influence
compositions
species,
hypothesized
changes
would
available
for
material
collection
thereby
affecting
microbiomes
their
environments.
anticipated
reduced
altered
increased
which
is
decrease
number
resources,
including
pool
floral
soil
bacteria
surrounding
environment.
As
expected,
observed
shifts
nests
across
varying
degrees
intensity,
differing
management
types
availability
flowers.
Shannon
(larval
pollen
provision,
enclosure)
guts
decreased
increasing
intensity.
pupae
remained
unaffected,
indicating
reorganization
during
metamorphosis,
not
significantly
influenced
resources.
Our
findings
provide
new
insights
into
shaping
environmental
transmission
microbiomes.
This
understanding
comprehending
impacts
intensive
developing
strategies
mitigate
effects.
Язык: Английский
Bee Microbiomes: Unveiling Diversity and Social Dynamics in Managed and Wild Bees
Опубликована: Янв. 1, 2025
Язык: Английский
Native bee habitat restoration: key ecological considerations from recent North American literature
Frontiers in Ecology and Evolution,
Год журнала:
2024,
Номер
12
Опубликована: Авг. 5, 2024
Habitat
loss
is
a
primary
driver
of
global
biodiversity
decline,
negatively
impacting
many
species,
including
native
bees.
One
approach
to
counteract
the
consequences
habitat
through
restoration,
which
includes
transformation
degraded
or
damaged
habitats
increase
biodiversity.
In
this
review,
we
survey
bee
restoration
literature
over
last
14
years
provide
insights
into
how
best
promote
diversity
and
abundance
natural
landscapes
in
North
America.
We
highlight
relevant
questions
concepts
consider
throughout
various
stages
projects,
categorizing
them
pre-,
during-,
post-restoration
stages.
emphasize
importance
planning
species-
site-specific
strategies
support
bees,
providing
floral
non-floral
resources
increasing
nest
site
availability.
Lastly,
underscore
significance
conducting
evaluations
long-term
monitoring
following
efforts.
By
identifying
effective
methods,
success
indicators,
areas
for
future
research,
our
review
presents
comprehensive
framework
that
can
guide
land
managers
during
urgent
time
restoration.
Язык: Английский
Development of a roadmap for action on the application of Omics and associated Bioinformatics Approaches in Risk Assessment
EFSA Supporting Publications,
Год журнала:
2024,
Номер
21(10)
Опубликована: Окт. 1, 2024
Abstract
The
implementation
of
omics
technologies
and
associated
bioinformatics
approaches
hold
significant
promise
for
generating
additional
evidence
food
feed
risk
assessments
thereby
enhancing
the
European
Food
Safety
Authority
(EFSA)
capacity
to
deliver
scientific
opinions
guidance
documents
in
future.
To
explore
this
possibility,
EFSA
launched
a
Call
development
roadmap
identify
main
actions
needed
wider
use
Omics
future
assessments.
address
objective,
action
outlines
six
project
proposals.
These
proposals
are
based
on
comprehensive
mapping
state‐of‐the‐art
research,
EFSA's
activities
as
well
current
planned
from
other
relevant
regulatory
bodies
organisations.
outlined
recommendations
also
some
identified
knowledge
gaps
highlight
added
value
that
further
investments
different
&
safety
domains
could
bring.
In
addition,
work
addresses
key
challenges
blockers
might
hinder
integration
assessment
leverages
opportunities
cooperation
with
external
stakeholders.
Finally,
provides
suggestions
how
may
more
broadly
effectively
engage
stakeholders
science.
Язык: Английский
Wild bee and pollen microbiomes across an urban–rural divide
FEMS Microbiology Ecology,
Год журнала:
2023,
Номер
99(12)
Опубликована: Ноя. 13, 2023
Abstract
Wild
pollinators
and
their
microbiota
are
sensitive
to
land
use
changes
from
anthropogenic
activities
that
disrupt
landscape
environmental
features.
As
urbanization
agriculture
affect
bee
habitats,
human-led
disturbances
driving
in
microbiomes,
potentially
leading
dysbiosis
detrimental
fitness.
This
study
examines
the
bacterial,
fungal,
plant
compositions
of
small
carpenter
bee,
Ceratina
calcarata,
its
pollen
provisions
across
an
urban–rural
divide.
We
performed
metabarcoding
C.
calcarata
Toronto
by
targeting
16S
rRNA,
ITS,
rbcL
regions.
Despite
similar
composition
diversity
bees
provisions,
there
was
a
greater
microbial
than
bees.
By
characterizing
differences
use,
climate,
pesticide
residues
differentiate
urban
rural
landscapes,
we
find
areas
support
elevated
levels
more
complex
networks
between
microbes
plants
areas.
However,
may
lead
lower
relative
abundances
known
beneficial
symbionts
increased
pathogens,
such
as
Ascosphaera
Alternaria
fungi.
Further,
indicate
dysregulate
symbiosis.
continue
alter
ever
changing
environments
threaten
crucial
maintaining
health.
Язык: Английский
Microbiome and floral associations of a wild bee using biodiversity survey collections
Environmental Microbiology,
Год журнала:
2024,
Номер
26(6)
Опубликована: Май 30, 2024
Abstract
The
health
of
bees
can
be
assessed
through
their
microbiome,
which
serves
as
a
biomarker
indicating
the
presence
both
beneficial
and
harmful
microorganisms
within
bee
community.
This
study
presents
characterisation
bacterial,
fungal,
plant
composition
on
cuticle
adult
bicoloured
sweat
(
Agapostemon
virescens
).
These
were
collected
using
various
methods
such
pan
traps,
blue
vane
traps
sweep
netting
across
northern
extent
habitat
range.
Non‐destructive
employed
to
extract
DNA
from
whole
pinned
specimens
these
wild
bees.
Metabarcoding
16S
rRNA,
ITS
rbcL
regions
was
then
performed.
found
that
method
collection
influenced
detection
certain
microbial
taxa.
Among
methods,
net
samples
showed
lowest
fungal
alpha
diversity.
However,
minor
differences
in
bacterial
or
beta
diversity
suggest
no
single
is
significantly
superior
others.
Therefore,
combination
techniques
cater
broader
spectrum
detection.
also
revealed
regional
variations
core
microbiome
A.
comprises
two
bacteria,
three
fungi
association,
all
are
commonly
detected
other
microbes
remained
consistent
different
locations.
Further
extensive
studies
microbiomes
species
landscapes
will
help
uncover
crucial
relationships
between
pollinator
environment.
Язык: Английский
Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 28, 2024
Abstract
The
global
decline
in
biodiversity
and
insect
populations
highlights
the
urgent
to
conserve
ecosystem
functions,
such
as
plant
pollination
by
solitary
bees.
Human
activities,
particularly
agricultural
intensification,
pose
significant
threats
these
essential
services.
Changes
land
use
alter
resource
nest
site
availability,
pesticide
exposure
other
factors
impacting
richness,
diversity,
health
of
bee
species.
In
this
study,
we
investigated
yet
another
facet
currently
less
well
context:
Microbial
communities
associated
with
wild
bees
play
crucial
roles
larval
development,
metabolism,
immunity
overall
health.
However,
drivers
dynamics
healthy
microbiome
are
still
poorly
understood,
especially
regarding
direct
indirect
effects
on
diversity
composition
microbial
communities.
We
examined
bacterial
offspring
materials
Megachilid
trap-nesting
bee,
O.
bicornis,along
a
gradient
intensification
16S
rRNA
gene
metabarcoding.
Given
that
landscape
composition,
climatic
conditions,
food
resources
known
influence
compositions
species,
hypothesized
changes
would
available
for
material
collection
thereby
affecting
microbiomes
their
environments.
anticipated
reduced
altered
increased
which
is
decrease
number
resources,
including
pool
floral
soil
bacteria
surrounding
environment.
As
expected,
observed
shifts
nests
across
varying
degrees
intensity,
differing
management
types
availability
flowers.
Shannon
(larval
pollen
provision,
enclosure)
guts
decreased
increasing
intensity.
pupae
remained
unaffected,
indicating
reorganization
during
metamorphosis,
not
significantly
influenced
resources.
Our
findings
provide
new
insights
into
shaping
environmental
transmission
microbiomes.
This
understanding
comprehending
impacts
intensive
developing
strategies
mitigate
effects.
Язык: Английский
New ecological insights on wild pollinator Andrena hesperia
Journal of Pollination Ecology,
Год журнала:
2024,
Номер
37, С. 303 - 325
Опубликована: Дек. 13, 2024
A
nesting
aggregation
of
Andrena
hesperia
in
Bologna
(Italy)
was
studied
to
characterize
this
bee’s
ecology,
phenology
and
interactions
with
the
environment.
adults
emerged
between
end
March
middle
April,
displaying
univoltine,
protandrous
phenology.
The
average
resistance
heat
stupor
A.
females
42.53
±
13.77
minutes
at
40°C.
Parasites
Nomada
facilis
Bombylius
canescens
were
associated
nests.
DNA
barcode
sequences
(COI
gene)
N.
sequenced
deposited
GenBank.
gut
microbiota
newly
individuals
dominated
by
Bacillota
(Lactobacillus
Fructobacillus)
Pseudomonadota
(Snodgrassella
alvei
Gilliamella).
pollen
carried
identified
morphologically
as
belonging
for
most
part
Asteraceae
family.
pathogens
detected
on
showed
different
infection
loads
newly-emerged
foraging
adults.
This
is
first
time
that
comprehensive
information
reported,
it
will
hopefully
foster
further
studies
wild
bee.
Язык: Английский