Fungal Biology, Год журнала: 2025, Номер 129(5), С. 101596 - 101596
Опубликована: Май 20, 2025
Язык: Английский
Virulence, Год журнала: 2020, Номер 11(1), С. 365 - 380
Опубликована: Апрель 7, 2020
Subtilisin-like Pr1 proteases of insect-pathogenic fungi are a large family extracellular cuticle-degrading enzymes that presumably determine capability hyphal invasion into insect hemocoel through normal cuticle infection, but remain poorly understood although often considered as virulence factors for genetic improvement fungal potential against pests. Here, we report not all 11 members necessarily function in Beauveria bassiana, an ancient wide-spectrum pathogen evolved pathogenicity ~200 million years ago. These phylogenetically similar to or distinct from homologues (Pr1A-K) early named Metarhizium anisopliae complex, young entomopathogen lineage undergoing molecular evolution toward diversification, and hence renamed Pr1A1/A2, Pr1B1-B3, C, Pr1F1-F4,4 G, respectively. Multiple analyses single gene-deleted rescued mutants led the recognition five conserved (Pr1C, Pr1G, Pr1A2, Pr1B1, Pr1B2) contributing significantly insect. The were proven only degradation, individually contribute 19-29% virulence, play no role post-infection cellular events critical killing action. Six other functional at either degradation during host infection virulence-related post-infection. Therefore, collectively required for, mark of, B. bassiana. findings provide first referable base insight different lineages pathogens.
Язык: Английский
Процитировано
78
Applied Microbiology and Biotechnology, Год журнала: 2018, Номер 103(2), С. 577 - 587
Опубликована: Ноя. 17, 2018
Язык: Английский
Процитировано
66
Postharvest Biology and Technology, Год журнала: 2025, Номер 222, С. 113425 - 113425
Опубликована: Янв. 31, 2025
Язык: Английский
Процитировано
1
Frontiers in Microbiology, Год журнала: 2025, Номер 16
Опубликована: Фев. 13, 2025
Increasing concerns about chemical fungicides require sustainable alternatives for crop protection. Microbe-mediated synthesis of metal nanoparticles offers a sustainable, eco-friendly and highly effective strategy plant disease management. This study investigates the mycosynthesis zinc oxide (ZnO NPs) using culture filtrate Trichoderma harzianum their antifungal activity against Alternaria brassicae . Nanoparticles were synthesized under optimized conditions cell-free (CFCF) concentration, substrate pH temperature. Ultraviolet-visible (UV-Vis) spectroscopy confirmed an absorption peak between 200 400 nm, while X-ray diffraction (XRD) confirms hexagonal crystal structure with average size 29 nm. Dynamic light scattering (DLS) zeta potential analysis revealed hydrodynamic 50.79 nm surface charge −17.49 mV, indicating good stability. Fourier transform infrared (FTIR) identified functional groups (C=O, N-O, O-H) that are crucial stabilization. Scanning electron microscopy (SEM) High-resolution transmission (HR-TEM) spherical, rod-shaped sizes 12 41 Mycogenic-zinc (M-ZnO significantly inhibited mycelial growth A. by 91.48% at μg/mL, compared to chemically ZnO NPs μg/mL (79.62%) mancozeb 0.2% (82.96%). SEM-EDX deformations M-ZnO in fungal hyphae, confocal laser scanning (CLSM) showed increased reactive oxygen species (ROS) formation impaired membrane integrity treated cells. Stress enzyme superoxide dismutase (SOD) catalase (CAT) 44.2 U/mol 39.6 NPs. Our studies suggest T. have even lower doses can be used as alternative traditional without affecting environment.
Язык: Английский
Процитировано
1
Virulence, Год журнала: 2018, Номер 10(1), С. 429 - 437
Опубликована: Сен. 27, 2018
Autophagy is a conserved self-degradation mechanism that governs large array of cellular processes in filamentous fungi. Filamentous insect and nematode mycopthogens function the natural control host populations have been widely applied for biological pests. Entomopathogenic nematophagous fungi "core" autophagy machineries are analogous to those found yeast but also feature several proteins involved specific aspects autophagic pathways. Here, we review functions protecting fungal cells from starvation stress cues sustaining cell differentiation, asexual development virulence. An emphasis placed upon regulatory mechanisms non-autophagic roles some autophagy-related genes. Methods used monitoring or events pathogens discussed.
Язык: Английский
Процитировано
47
Journal of environmental chemical engineering, Год журнала: 2019, Номер 7(3), С. 103193 - 103193
Опубликована: Июнь 1, 2019
Язык: Английский
Процитировано
45
Applied Microbiology and Biotechnology, Год журнала: 2020, Номер 104(13), С. 5711 - 5724
Опубликована: Май 13, 2020
Язык: Английский
Процитировано
42
Pest Management Science, Год журнала: 2021, Номер 78(1), С. 30 - 42
Опубликована: Авг. 16, 2021
Abstract Resistance to solar ultraviolet (UV) irradiation is crucial for field‐persistent control efficacies of fungal formulations against arthropod pests, because their active ingredients are formulated conidia very sensitive UV wavelengths. This review seeks summarize advances in studies aiming quantify, understand and improve conidial resistance. One focus has been on the many sets genes that have revealed postgenomic era contribute or mediate resistance insect pathogens serving as main sources insecticides. Such genetic unveiled broad basis UV‐resistant molecules including cytosolic solutes, cell wall components, various antioxidant enzymes, numerous effectors signaling proteins, function developmental, biosynthetic stress‐responsive pathways. Another molecular regulatory mechanisms underlying photorepair UV‐induced DNA lesions photoreactivation UV‐impaired conidia. Studies shed light upon a photoprotective mechanism depending not only one two photorepair‐required photolyases, but also white collar proteins other partners play similar more important roles via interactions with photolyases. Research hotspots suggested explore network photoprotection development application strategies © 2021 Society Chemical Industry.
Язык: Английский
Процитировано
40
Environmental Microbiology, Год журнала: 2019, Номер 21(12), С. 4648 - 4661
Опубликована: Авг. 21, 2019
Summary The APSES protein family comprises a conserved class of fungus‐specific transcriptional regulators. Some members have been identified in partial ascomycetes. In this study, the StuA (AoStuA) nematode‐trapping fungus Arthrobotrys oligospora was characterized. Compared with wild‐type (WT) strain, three Δ AoStuA mutants grew relatively slowly, displayed 96% reduction sporulation capacity and delay conidial germination. reduced correlated repression several sporulation‐related genes. were also more sensitive to chemical stressors than WT strain. Importantly, unable produce mycelial traps for nematode predation. Moreover, peroxisomes Woronin bod ies abundant cells but hardly found those mutants. lack such organelles some genes involved biogenesis ies. transcript levels cAMP/PKA signalling pathway significantly versus implicating regulatory role transcription that regulates an array cellular processes events. particular, is indispensable A . trap formation virulence.
Язык: Английский
Процитировано
43
Applied and Environmental Microbiology, Год журнала: 2018, Номер 85(4)
Опубликована: Дек. 12, 2018
Protecting fungal cells from damage solar UVB irradiation is critical for development and application of insecticides but mechanistically not understood in Beauveria bassiana , a classic insect pathogen. We unveil that two intranuclear photolyases, Phr1 Phr2, play essential roles repairing UVB-induced dsDNA lesions through respective decomposition cytotoxic cyclobutane pyrimidine dimers (6-4)-pyrimidine-pyrimidine photoproducts, hence reactivating UVB-inactivated effectively under visible light. Our findings shed light on the high potential both photolyase genes use improving resistance strategy insecticides.
Язык: Английский
Процитировано
42