Microorganisms,
Год журнала:
2023,
Номер
11(12), С. 2914 - 2914
Опубликована: Дек. 3, 2023
The
exposure
of
microorganisms
to
conventional
plastics
is
a
relatively
recent
occurrence,
affording
limited
time
for
evolutionary
adaptation.
As
part
the
EU-funded
project
BioICEP,
this
study
delves
into
plastic
degradation
potential
isolated
from
sites
with
prolonged
pollution,
such
as
plastic-polluted
forests,
biopolymer-contaminated
soil,
oil-contaminated
municipal
landfill,
but
also
distinctive
soil
sample
pieces
buried
three
decades
ago.
Additionally,
samples
Arthropoda
species
were
investigated.
In
total,
150
strains
and
screened
ability
use
plastic-related
substrates
(Impranil
dispersions,
polyethylene
terephthalate,
terephthalic
acid,
bis(2-hydroxyethyl)
terephthalate).
Twenty
isolates
selected
based
on
their
grow
various
identified
Streptomyces,
Bacillus,
Enterococcus,
Pseudomonas
spp.
Morphological
features
recorded,
16S
rRNA
sequence
was
employed
construct
phylogenetic
tree.
Subsequent
assessments
unveiled
that
5
out
20
displayed
capability
produce
polyhydroxyalkanoates,
utilizing
pre-treated
post-consumer
PET
samples.
With
Priestia
sp.
DG69
Neobacillus
DG40
emerging
most
successful
producers
(4.14%
3.34%
PHA,
respectively),
these
are
poised
further
utilization
in
upcycling
purposes,
laying
foundation
development
sustainable
strategies
waste
management.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 30, 2025
Introduction
Di-n-butyl
phthalate
(DBP)
is
one
of
the
most
widely
used
esters
(PAEs)
and
considered
an
emerging
global
pollutant.
It
may
pose
a
significant
threat
to
ecosystem
human
health
due
its
residual
hazards
accumulation
in
environment.
Bacteria-driven
PAE
biodegradation
economical
effective
strategy
for
remediating
such
polluted
environments.
Methods
A
DBP-degrading
bacterium
(P-7),
was
isolated
from
long-term
film
mulched
cotton
field
soil.
Its
identity
confirmed
via
physiological,
biochemical,
16S
rRNA
gene
analyses.
The
degradation
conditions
were
optimized
through
single-factor
experiments
response
surface
methodology
(RSM).Furthermore,
whole-genome
sequencing
coupled
with
metabolomics
employed
elucidate
metabolic
mechanisms.
Results
Priestia
megaterium
P-7
(
P.
P-7)
achieved
100%
DBP
removal
within
20
h
under
optimal
exhibited
broad
substrate
specificity
other
PAEs.
Genomic
analysis
identified
key
genes
lip
,
aes
ybfF
estA
yvaK
)
encoding
esterases/hydrolases
that
initiate
catabolism,
converting
it
phthalic
acid
(PA).
Subsequent
decarboxylation
pdc
bsdCD
mdcACDH
lysA
dioxygenase-mediated
steps
integrated
PA
into
TCA
cycle.
Metabolomics
revealed
three
pathways:
(DBP
→
MBP
BB
BA→Catechol),
hydrolysis
PCA
Catechol)
direct
β-oxidation
DEP
MEP
Catechol).
Conclusion
demonstrates
exceptional
efficiency,
versatility,
environmental
stress
tolerance,
making
promising
candidate
bioremediation
organic
pollutants
contaminated
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(2), С. 1302 - 1320
Опубликована: Янв. 4, 2024
Inulin
is
widely
used
as
a
prebiotic
and
emerging
priming
compound
to
counteract
plant
diseases.
We
isolated
inulin-degrading
strains
from
the
lettuce
phyllosphere,
identified
Bacillus
subtilis
Priestia
megaterium,
species
hosting
well-known
biocontrol
organisms.
To
better
understand
their
varying
inulin
degradation
strategies,
three
intracellular
β-fructofuranosidases
P.
megaterium
NBRC15308
were
characterized
after
expression
in
Escherichia
coli:
predicted
sucrose-6-phosphate
(Suc6P)
hydrolase
(SacAP1,
supported
by
molecular
docking),
an
exofructanase
(SacAP2),
invertase
(SacAP3).
Based
on
protein
multiple
sequence
structure
alignments
of
bacterial
glycoside
family
32
enzymes,
we
conserved
residues
be
involved
binding
phosphorylated
(Suc6P
hydrolases)
or
nonphosphorylated
substrates
(invertases
fructanases).
Suc6P
hydrolases
feature
positively
charged
near
structural
catalytic
pocket
(histidine,
arginine,
lysine),
whereas
other
contain
tryptophans.
This
correlates
with
our
phylogenetic
tree,
grouping
all
clan
associated
genomic
regions
coding
for
transporters
substrate
phosphorylation.
These
results
will
help
discriminate
between
future
studies
interaction
B.
endophytes
sucrose
and/or
fructans,
sugars
naturally
present
plants
exogenously
applied
context
defense
priming.
Microorganisms,
Год журнала:
2023,
Номер
11(12), С. 2914 - 2914
Опубликована: Дек. 3, 2023
The
exposure
of
microorganisms
to
conventional
plastics
is
a
relatively
recent
occurrence,
affording
limited
time
for
evolutionary
adaptation.
As
part
the
EU-funded
project
BioICEP,
this
study
delves
into
plastic
degradation
potential
isolated
from
sites
with
prolonged
pollution,
such
as
plastic-polluted
forests,
biopolymer-contaminated
soil,
oil-contaminated
municipal
landfill,
but
also
distinctive
soil
sample
pieces
buried
three
decades
ago.
Additionally,
samples
Arthropoda
species
were
investigated.
In
total,
150
strains
and
screened
ability
use
plastic-related
substrates
(Impranil
dispersions,
polyethylene
terephthalate,
terephthalic
acid,
bis(2-hydroxyethyl)
terephthalate).
Twenty
isolates
selected
based
on
their
grow
various
identified
Streptomyces,
Bacillus,
Enterococcus,
Pseudomonas
spp.
Morphological
features
recorded,
16S
rRNA
sequence
was
employed
construct
phylogenetic
tree.
Subsequent
assessments
unveiled
that
5
out
20
displayed
capability
produce
polyhydroxyalkanoates,
utilizing
pre-treated
post-consumer
PET
samples.
With
Priestia
sp.
DG69
Neobacillus
DG40
emerging
most
successful
producers
(4.14%
3.34%
PHA,
respectively),
these
are
poised
further
utilization
in
upcycling
purposes,
laying
foundation
development
sustainable
strategies
waste
management.
