Identification of protein-degraders in an anaerobic digester by protein stable isotope probing combined with metagenomics DOI Creative Commons
Zhe Deng, Jan Struckmann Poulsen, Jeppe Lund Nielsen

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Июнь 22, 2023

Abstract BackgroundPresence of carbohydrates hampers protein degradation in anaerobic digesters. To understand this phenomenon, we used proteogenomics to identify the active protein-degraders presence low and high concentrations. Active metabolic pathways identified were investigated using proteomics with13C-protein substrates (protein stable isotope probing).ResultsAcinetobacterwas as main under both protein-fed protein-glucose mixture-fed conditions. The incorporation the13C-labelled substrate was predominantly observed outer membrane-bound proteins porin proteins, which are associated with proteinases or transportation amino acids across cell wall. TheAcinetobactermetabolic model incubation conditions suggested that glucose degraded through respiration.ConclusionsThe unfavourable effect on biodegradation attributed toAcinetobacter’s preference for substrate. This work highlights efficient carbohydrate mixtures digesters requires a staged time-phased approach enrichment protein-degraders.

Язык: Английский

Alteration of shoaling behavior and dysbiosis in the gut of medaka (Oryzias latipes) exposed to 2-μm polystyrene microplastics DOI

Yui Tamura,

Yuki Takai, Hirokuni Miyamoto

и другие.

Chemosphere, Год журнала: 2024, Номер 353, С. 141643 - 141643

Опубликована: Март 4, 2024

Язык: Английский

Процитировано

7
Identification of protein-degraders in an anaerobic digester by protein stable isotope probing and metagenomics DOI Creative Commons
Zhe Deng, Jan Struckmann Poulsen, Jeppe Lund Nielsen

и другие.

Applied Microbiology and Biotechnology, Год журнала: 2025, Номер 109(1)

Опубликована: Апрель 10, 2025

Язык: Английский

Процитировано

0
In anaerobic reactors the microbial community structure depends on feed type, with no “keystone” species tied to COD removal DOI Creative Commons

Olutooni Ajayi,

William A. Arnold, Natasha C. Wright

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Май 14, 2025

Two-stage anaerobic digestion (AD) systems provide treatment for high strength wastewater with stability and performance. Encapsulation technology can intensify AD to facilitate the separation of solids retention time from hydraulic (HRT), offering lower HRTs, smaller reactors, effluent quality. To support successful deployment, however, encapsulated community must contain all needed microorganisms be flexible enough treat a variety wastewaters. Here, two-stage system was investigated in which microbial cultures were enriched on various high-strength wastewaters suspended flow-through determine how feed type influenced performance structure. The hypothesis that specific genera, or so-called "keystone species" would positively correlate organic carbon degradation given feed, enabling construction well-functioning encapsulation. Results showed number total bacteria (as 16S rRNA gene copies) did not soluble chemical oxygen demand (sCOD) removal, indicating structure and/or members important good also strongly removal 1st-stage fermenting communities, but 2nd-stage methanogenic communities. In this study, "core" defined as organisms common either 1st- reactors irrespective they received present at least 50% samples throughout entire experiment. "Unique" single hence, only receiving feed. both one core genera no unique significantly correlated sCOD removal. Verification experiments performed communities identified degradation, though significantly, seemed Our results suggest cannot construct containing populations lieu enrichment. Nevertheless, diverse should (>80%) when fed influents, if is provided enrichment after deployment.

Язык: Английский

Процитировано

0
A novel additive for enhancing biomass energy production from agro-industrial wastes: synthesis of hydrophobic nanoporous silica aerogel and its effect on methane production DOI Creative Commons
Habibe Elif Gülşen Akbay, Öyküm Başgöz, Ömer Güler

и другие.

Biomass Conversion and Biorefinery, Год журнала: 2024, Номер 15(5), С. 7203 - 7222

Опубликована: Апрель 13, 2024

Abstract Anaerobic digestion (AD) is one of the most preferred processes for treatment organic waste. However, additional such as co-digestion, pretreatment, and additive addition continue to be explored remove limits on applicability AD. This study investigated effects hydrophobic nanoporous silica aerogel (NpSA) synthesized from waste rice husks anaerobic co-digestion (AnCD) mixture consisting sewage sludge fruit processing industry wastes. All bioreactors containing NpSA-free, 0.1 g, 0.2 0.5 1 g NpSA (0.03–0.3 /gVS added ) were operated in a mesophilic-batch process. Biogas methane yields increased 346 mL/gVS (NpSA-free) 387 231 288 mL/gVS, respectively, with addition. biogas production all compared blank. rate content faster at lower doses NpSA. Maximum soluble chemical oxygen demand (sCOD), protein, carbohydrate, volatile solid (VS) reductions between 45–71%, 35–54%, 44–65%, 34–91% mixtures, respectively. The was effective improving AnCD performance biogas/methane production. Experimental results fit kinetic models frequently AD processes. In addition, possible energy financial potential produced also discussed, it determined that direct sale gas by global market could provide 1.4 $/L more gain than NpSA-free. Graphical

Язык: Английский

Процитировано

1
Automatización de un digestor anaerobio con sistema embebido para la producción de biogás a partir de residuo del aceite de palma DOI Open Access
Carlos Arturo Vides-Herrera, Aldo Pardo García, Adalberto Ospino Castro

и другие.

REVISTA COLOMBIANA DE TECNOLOGIAS DE AVANZADA, Год журнала: 2024, Номер 2(44), С. 65 - 73

Опубликована: Июль 6, 2024

Este trabajo presenta la automatización de un reactor anaerobio utilizado para producción biogás a partir lodos residuales en el proceso extracción del aceite palma (POME). El se realiza utilizando sistemas embebidos como tarjeta Arduino DUE. Las variables que miden este son temperatura, PH y agitación; parámetros más críticos controlar afectan significativamente calidad mismo. Para utilizaron sensores determinar cantidad metano (CH4), dióxido carbono (CO2) ácido sulfhídrico (H2S). adquisición data utilizó comunicación USB entre embebida PC, Visual Basic.NET, sistema adquisición, almacenamiento visualización datos las biodigestión POME, mide producido tiempo real visualiza almacena información posterior análisis estadístico.

Процитировано

1
De novo anaerobic granulation with varying organic substrates: Granule growth and microbial community responses DOI Creative Commons
Chang Gao, Anna Doloman,

Emilie Alaux

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 951, С. 175570 - 175570

Опубликована: Авг. 15, 2024

Anaerobic granulation from dispersed inoculum is recognized as a slow process. However, studies under saline conditions have shown that adding complex proteinaceous substrates can accelerate this To explore whether holds true also non-saline conditions, we conducted 262-days experiment with four lab-scale upflow anaerobic sludge blanket reactors inoculated digested sewage sludge. Each reactor received synthetic feed containing varying amount of carbohydrate/protein substrate: glucose (RGlu), acetate/tryptone (RAc+Try), glucose/tryptone (RGlu+Try), and glucose/starch (RGlu+Sta). Development granules different influent composition was monitored macroscopy, analysis the extracellular polymeric substances, microbial diversity. Granulation faster in RGlu+Try RGlu+Sta. Increasing granule diameters positively correlated occurrence bacteria Muribaculaceae Lachnospiraceae families, suggesting their involvement de novo granulation. Granules had high relative abundances both fermenting (e.g. Lactococcus, Streptococcus, Trichococcus) involved oxidation volatile fatty acids (Smithella, Acetobacteroides). The results study provide basis for strategies to enhance rate practice when granular not available. Specifically, supplementing small amounts waste protein during start-up be effective.

Язык: Английский

Процитировано

1
Tailored short-chain fatty acids conversion from waste activated sludge fermentation via persulfate oxidation and C3–C5 io-SRB metabolizers DOI

Huijie Tan,

Aijuan Zhou,

Lijun Jia

и другие.

Journal of Environmental Management, Год журнала: 2023, Номер 346, С. 118967 - 118967

Опубликована: Сен. 12, 2023

Язык: Английский

Процитировано

2
Identification of protein-degraders in an anaerobic digester by protein stable isotope probing combined with metagenomics DOI Creative Commons
Zhe Deng, Jan Struckmann Poulsen, Jeppe Lund Nielsen

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Июнь 22, 2023

Abstract BackgroundPresence of carbohydrates hampers protein degradation in anaerobic digesters. To understand this phenomenon, we used proteogenomics to identify the active protein-degraders presence low and high concentrations. Active metabolic pathways identified were investigated using proteomics with13C-protein substrates (protein stable isotope probing).ResultsAcinetobacterwas as main under both protein-fed protein-glucose mixture-fed conditions. The incorporation the13C-labelled substrate was predominantly observed outer membrane-bound proteins porin proteins, which are associated with proteinases or transportation amino acids across cell wall. TheAcinetobactermetabolic model incubation conditions suggested that glucose degraded through respiration.ConclusionsThe unfavourable effect on biodegradation attributed toAcinetobacter’s preference for substrate. This work highlights efficient carbohydrate mixtures digesters requires a staged time-phased approach enrichment protein-degraders.

Язык: Английский

Процитировано

0