Industrial Crops and Products, Год журнала: 2024, Номер 222, С. 119673 - 119673
Опубликована: Сен. 19, 2024
Язык: Английский
Fermentation, Год журнала: 2025, Номер 11(3), С. 156 - 156
Опубликована: Март 20, 2025
C4-dicarboxylic acids are essential organic compounds characterized by a four-carbon structure and two carboxyl groups. Their export from cells is mediated specialized transporter proteins known as acid transporters (DCTs). The objective of this study was to investigate the specificity six DCTs (DCT1-5 C4t318) Aspergillus niger or oryzae, focusing on their role in different production strategies for acids. results indicate that compared WT strain, overexpressing dct2 dct3 A. CGMCC NO. 40550 specifically enhances succinic acid, increasing its yield 5.69 g/L 6.28 g/L, L-malic 11.02 12.11 g/L. Additionally, dct5 appears be involved transport both (6.19 g/L) (16.33 g/L). total yields T-D3-7, which lacks oxaloacetate hydrolase gene, were improved 27.75 25.19 T-D3-26, due blocking branch metabolism. Furthermore, heterologous expression oryzae C4T318 increased approximately 22.5%. best observed when strains T-D3-7 T-D5-16 scaled up 30 L bioreactor 84 h. reached 14.51 70.79 41.59 81.08 respectively. Moreover, purity produced 71%. This work further clarifies provides valuable insights optimizing production.
Язык: Английский
Процитировано
0
Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Carotenoids and apocarotenoids, natural compounds with vital biological functions, are now sustainably produced via microbial synthesis as an eco-friendly alternative to inefficient polluting traditional plant-based extraction methods. In their biosynthesis, β-carotene (BC) plays a crucial role it is the key intermediate from which different downstream derivatives formed. Here, we engineered high-producing Saccharomyces cerevisiae platform strain produce BC through combination of systematic metabolic engineering atmospheric room temperature plasma mutagenesis. The achieved production 2.09 g/L fed-batch fermentation in 5-L bioreactor, highest yield reported S. date. Using this strain, constructed zeaxanthin- β-ionone-producing strains by introducing enzyme genes. 39.09 mg/L zeaxanthin 31.87 β-ionone shake-flask cultures. established study provides higher starting point for producing diverse derivatives.
Язык: Английский
Процитировано
0
Fermentation, Год журнала: 2025, Номер 11(5), С. 263 - 263
Опубликована: Май 6, 2025
The aim of this work is to study the metabolism Actinobacillus succinogenes in greater detail with optimizing succinate production and creating a metabolic model. inhibitory properties various substances were first investigated. It was found that nature availability gas can have strong influence on metabolism. By studying effects different sources, it when A. lacks CO2 source, completely switches C3 pathway. This also changes path within In presence CO2, significantly more formate (2.44 ± 0.04 g L−1) less acetate (1.63 0.03 produced. contrast, absence concentration 1.94 0.12 L−1, 2.73 0.15 L−1. addition, larger amounts ethanol (1.34 0.28 produced whereas hardly any otherwise. All these results show that, organism has regenerate much NADH NAD+ via subsequent investigation an increase product from 1.55 0.13 L−1 6.11 0.09 achieved by combining gaseous NaHCO3. shown microaerobic environment not sufficient towards lactate formation. Using model, possible verify main pathways observed during experimental bioreactor runs 2-L scale. conducting further modification, now use model predict external electron supply redox
Язык: Английский
Процитировано
0
Industrial Crops and Products, Год журнала: 2024, Номер 222, С. 119673 - 119673
Опубликована: Сен. 19, 2024
Язык: Английский
Процитировано
2