Unusual depolymerization mechanism of Poly(ethylene terephthalate) by hydrolase 202

Chemosphere, Год журнала: 2025, Номер 372, С. 144108 - 144108

Опубликована: Янв. 15, 2025

Язык: Английский

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Microplastic accumulation in soils: Unlocking the mechanism and biodegradation pathway

Journal of Hazardous Materials Advances, Год журнала: 2025, Номер 17, С. 100629 - 100629

Опубликована: Фев. 1, 2025

Язык: Английский

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Biocatalytic recycling of plastics: facts and fiction

Chemical Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Enzymatic recycling of plastic waste is attracting considerable attention as a novel strategy to advance circular economy. While currently limited polyesters, achieving economically viable biocatalytic processes remains key challenge.

Язык: Английский

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Plastic-Degrading Enzymes from Marine Microorganisms and Their Potential Value in Recycling Technologies

Marine Drugs, Год журнала: 2024, Номер 22(10), С. 441 - 441

Опубликована: Сен. 26, 2024

Since the 2005 discovery of first enzyme capable depolymerizing polyethylene terephthalate (PET), an aromatic polyester once thought to be enzymatically inert, extensive research has been undertaken identify and engineer new biocatalysts for plastic degradation. This effort was directed toward developing efficient enzymatic recycling technologies that could overcome limitations mechanical chemical methods. These enzymes are versatile molecules obtained from microorganisms living in various environments, including soil, compost, surface seawater, extreme habitats such as hot springs, hydrothermal vents, deep-sea regions, Antarctic seawater. Among plastics, PET polylactic acid (PLA) have primary focus depolymerization research, greatly enhancing our knowledge degrade these specific polymers. They often display unique catalytic properties reflect their particular ecological niches. review explores recent advancements marine-derived can depolymerize synthetic polymers, emphasizing structural functional features influence efficiency catalysts biorecycling processes. Current status future perspectives also discussed, with a on underexplored marine resources.

Язык: Английский

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Structure and Energetics of PET-Hydrolyzing Enzyme Complexes: A Systematic Comparison from Molecular Dynamics Simulations

Journal of Chemical Information and Modeling, Год журнала: 2024, Номер 64(21), С. 8236 - 8257

Опубликована: Окт. 21, 2024

Discovered in 2016, the enzyme PETase, secreted by bacterial Ideonella Sakaiensis 201-F6, has an excellent hydrolytic activity toward poly(ethylene terephthalate) (PET) at room temperature, while it decreases higher temperatures due to low thermostability. Many variants have been engineered overcome this limitation, which hinders industrial application. In work, we systematically compare PETase wild-type (WT) and four mutants (DuraPETase, ThermoPETase, FastPETase, HotPETase) using standard molecular dynamics (MD) simulations unbinding free energy calculations. particular, analyze enzymes' structural characteristics binding a tetrameric PET chain (PET4) under two temperature conditions: T1─300 K T2─350 K. Our results indicate that (i) PET4 forms stable complexes with five enzymes (∼300 K) (ii) most of interactions are localized close active site protein, where W185 Y87 residues interact aromatic rings substrate. Specifically, (iii) side-chain explores different conformations each variant (a phenomenon known literature as "W185 wobbling"). This suggests pocket retains plasticity flexibility among variants, facilitating substrate recognition localization events moderate temperatures. Moreover, (iv) establishes catalytic H237 residue, stabilizing triad composed S160-H237-D206, helping system achieve effective configuration for hydrolysis reaction. Conversely, (v) affinity (∼350 K), retaining only HotPETase. Finally, (vi) MD formed poly(ethylene-2,5-furan dicarboxylate) (PEF) show no persistent interactions, suggesting these not yet optimized alternative semiaromatic plastic polymer. study offers valuable insights into stability determinants driving onto their surfaces, sheds light on mechanistic steps precede onset hydrolysis, provides foundation future optimization.

Язык: Английский

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Hydrolysis rate and mechanism of water-dispersed polyesters with different sulfonate group contents in the cutinase-catalyzed system

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 283, С. 137775 - 137775

Опубликована: Ноя. 16, 2024

Язык: Английский

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