Genome-Guided Identification and Characterisation of Broad-Spectrum Antimicrobial Compounds of Bacillus velezensis Strain PD9 Isolated from Stingless Bee Propolis

Probiotics and Antimicrobial Proteins, Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Язык: Английский

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Endophytic Bacillus spp. of coffee plants (Coffea arabica L.) and its potential in the biocontrol of phytopathogenic fungi and Lepidoptera larvae

Egyptian Journal of Biological Pest Control, Год журнала: 2025, Номер 35(1)

Опубликована: Фев. 21, 2025

Abstract Background Research with coffee ( Coffea arabica L., Rubiaceae) endophytic microorganisms demonstrates that there is a rich microbiota has diverse abilities to protect plants and act against phytopathogens. Therefore, the isolation knowledge of are important for future explorations aimed at using promising strains in controlling pests diseases promoting plant growth. The present work to: (i) isolate identify Bacillus from leaves C. plants; (ii) carry out morphological molecular characterization; (iii) evaluate mycelial inhibition capacity soil phytopathogens vitro; (iv) pathogenicity tested lepidopteran insect pests. Results As result, three species were isolated: B. mycoides, thuringiensis velezensi s. was virulent Spodoptera frugiperda, Helicoverpa armigera, Chrysodeixis includens Diatraea saccharalis showed 100% pest mortality, lethal concentration (LC 50 values) estimated: 6.78 × 10 3 spores/ml S. frugiperda ; 6.23 2 H. armigera 9.28 5.81 1 D. . In total, seven genes related insects found based on genome: cry1Ab24, cry1Bd2, cry2Aa9, cry2Ab41, cry1Ea7, cry1Ia37 Spp1Aa1. Furthermore, velezensis able inhibit growth phytopathogens: Fusarium oxysporum (49%) , Rhizoctonia solan i (72%) Macrophomina phaseolina (65%) provided above 50% mortality Regarding mycoides it not efficient inhibiting pathogens causing mortality. Conclusion Two ) bacteria isolated potential use biological control agriculture, high larvae laboratory thurigiensis More studies these two must be carried greenhouse field prove its beneficial properties.

Язык: Английский

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Rhizobacteria and Phytohormonal interactions increase Drought Tolerance in Phaseolus vulgaris through enhanced physiological and biochemical efficiency

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Дек. 28, 2024

The cultivation of common beans (Phaseolus vulgaris L.) in semi-arid regions is affected by drought. To explore potential alleviation strategies, we investigated the impact inoculation with Bacillus velezensis, and application acetylsalicylic acid (ASA) via foliage (FA), which promote plant growth enhance stress tolerance. A split-split-plot experiment four replications was conducted, featuring two irrigation levels: full watering (FW, 100% water requirements) deficit (DW, 70% as a main plot, ASA levels (No (NFA) 0 0.5 mM) sub bacterial (BI) versus non-bacterial (NBI) sub-sub plot. Results showed that highest grain yield achieved + BI under FW (3270 kg ha−¹), 56% increase compared to control (2094 ha−¹). Under DW, increased approximately 30%. significantly relative content watering, achieving 84% BI. Chlorophyll peaked at 3.11 mg g− 1 chlorophyll b up 23.8% indicating improved photosynthetic capacity. Malondialdehyde hydrogen peroxide were reduced 10.88 14.81 µmol g−¹ fresh weight, respectively, treatments, demonstrating oxidative stress. Antioxidant enzyme activities elevated treated plants DW. This study demonstrates microbial hormonal treatments boosting drought tolerance beans, providing viable approach for sustaining crop performance conditions.

Язык: Английский

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Biocontrol efficacy of Botrytis cinerea on postharvest tomato fruit by the endophytic bacterium Bacillus velezensis BE1

Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер unknown, С. 102427 - 102427

Опубликована: Окт. 1, 2024

Язык: Английский

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