Establishing In Vitro Dosimetric Models and Dose–Effect Relationships for177Lu-DOTATATE in Neuroendocrine Tumors DOI
G Tamborino,

Pleun Engbers,

Tijmen H. de Wolf

и другие.

Journal of Nuclear Medicine, Год журнала: 2025, Номер unknown, С. jnumed.125.269470 - jnumed.125.269470

Опубликована: Май 22, 2025

This study investigates the radiobiology of peptide receptor radionuclide therapy (PRRT) using clinically relevant cancer cell lines by developing a framework for realistic cellular dosimetry in 2- and 3-dimensional cluster-forming configurations. Methods: The radiobiologic responses GOT1 NCI-H69 tumor to PRRT external beam radiotherapy (EBRT) were compared. Viability at 7 d death multiple time points assessed. Image-based multicellular models developed reflect vitro exposure complexity compared with traditional approaches. Results: absorbed dose suspension was dominated medium during incubation cross-dose within small clusters after incubation. Our findings reveal that can underestimate doses up 90% plated setups overlooks heterogeneity, initial rates varying 2.3-fold based on cluster size arrangement. maximum relative biologic effectiveness EBRT loss viability 0.43 ± 0.07 cells 0.22 0.02 cells. showed greater resistance PRRT-induced than EBRT, whereas exhibited similar levels both treatments, albeit different dose-response dynamics. Conclusion: requires average an 3 times higher achieve equivalent effects vitro. Traditional overestimates underestimating dose.

Язык: Английский

Establishing In Vitro Dosimetric Models and Dose–Effect Relationships for177Lu-DOTATATE in Neuroendocrine Tumors DOI
G Tamborino,

Pleun Engbers,

Tijmen H. de Wolf

и другие.

Journal of Nuclear Medicine, Год журнала: 2025, Номер unknown, С. jnumed.125.269470 - jnumed.125.269470

Опубликована: Май 22, 2025

This study investigates the radiobiology of peptide receptor radionuclide therapy (PRRT) using clinically relevant cancer cell lines by developing a framework for realistic cellular dosimetry in 2- and 3-dimensional cluster-forming configurations. Methods: The radiobiologic responses GOT1 NCI-H69 tumor to PRRT external beam radiotherapy (EBRT) were compared. Viability at 7 d death multiple time points assessed. Image-based multicellular models developed reflect vitro exposure complexity compared with traditional approaches. Results: absorbed dose suspension was dominated medium during incubation cross-dose within small clusters after incubation. Our findings reveal that can underestimate doses up 90% plated setups overlooks heterogeneity, initial rates varying 2.3-fold based on cluster size arrangement. maximum relative biologic effectiveness EBRT loss viability 0.43 ± 0.07 cells 0.22 0.02 cells. showed greater resistance PRRT-induced than EBRT, whereas exhibited similar levels both treatments, albeit different dose-response dynamics. Conclusion: requires average an 3 times higher achieve equivalent effects vitro. Traditional overestimates underestimating dose.

Язык: Английский

Процитировано

0