Fungal Biology,
Год журнала:
2024,
Номер
128(2), С. 1643 - 1656
Опубликована: Янв. 24, 2024
Microsclerotia
(MS)
are
considered
one
of
the
most
promising
propagules
for
use
as
active
ingredients
in
biopesticides
due
to
their
tolerance
abiotic
factors
and
ability
produce
infective
conidia
control
insect
pests
plant
pathogens.
Therefore,
main
objective
this
research
was
establish
conditions
required
induce
formation
microsclerotia
Metarhizium
robertsii
Mt004
study
its
development
process,
insecticidal
activity
MS-derived
conidia.
M.
started
form
hyphal
aggregates
after
2
days,
structures
looked
more
compact
8
MS
were
mature
pigmented
20
days.
The
final
yield
2.0
×
103
MS/mL,
size
varied
between
356.9
1348.4
μm.
Ultrastructure
analysis
revealed
that
internal
cells
lyse
during
maturation
contained
only
a
few
live
embedded
an
extracellular
matrix.
Conidia
produced
by
Solid
State
Fermentation
(SSF)
on
rice
completely
died
1
h
irradiation
with
UV-B
light,
while
84.7%
remained
viable
4
h.
2.5
times
tolerant
heat
than
conidia,
when
exposed
3
at
50
°C.
Storage
trials
showed
viability
rapidly
dropped
months,
months
storage
°C
18
virulent
grains
against
Diatraea
saccharalis
larvae.
Mature
radiation
from
SSF.
fermentation
process
requires
optimization
increase
reducing
time
maintaining
robustness.
In
general,
these
results
led
conclusion
persistent
efficient
harsh
environmental
conditions.
Our
findings
provide
baseline
production
better
understanding
strains.
Journal of Agricultural and Food Chemistry,
Год журнала:
2023,
Номер
71(36), С. 13244 - 13254
Опубликована: Авг. 30, 2023
Beauveria
bassiana
acts
as
an
endophytic
fungus
that
controls
herbivorous
pests
by
stimulating
plant
defenses
and
inducing
systemic
resistance.
Through
multiomics
analysis,
325
differential
metabolites
1739
expressed
genes
were
observed
in
tomatoes
treated
with
B.
root
irrigation;
meanwhile,
152
1002
local
leaf
spraying.
Among
the
upregulated
α-solanine,
5-O-caffeoylshikimic
acid,
clerodendrin
A,
peucedanin,
which
demonstrated
anti-insect
activity.
These
primarily
associated
alkaloid
biosynthesis,
flavonoid
tryptophan
metabolism
pathways.
Furthermore,
gene
silencing
of
UDP-glucose:sterol
glucosyltransferase,
a
involved
α-solanine
synthesis,
indicated
could
inhibit
reproduction
whiteflies
regulating
α-solanine.
This
study
highlighted
ability
to
modulate
secondary
emphasized
significance
understanding
harnessing
multitrophic
interactions
for
sustainable
agriculture.
Fungal Biology,
Год журнала:
2024,
Номер
128(2), С. 1643 - 1656
Опубликована: Янв. 24, 2024
Microsclerotia
(MS)
are
considered
one
of
the
most
promising
propagules
for
use
as
active
ingredients
in
biopesticides
due
to
their
tolerance
abiotic
factors
and
ability
produce
infective
conidia
control
insect
pests
plant
pathogens.
Therefore,
main
objective
this
research
was
establish
conditions
required
induce
formation
microsclerotia
Metarhizium
robertsii
Mt004
study
its
development
process,
insecticidal
activity
MS-derived
conidia.
M.
started
form
hyphal
aggregates
after
2
days,
structures
looked
more
compact
8
MS
were
mature
pigmented
20
days.
The
final
yield
2.0
×
103
MS/mL,
size
varied
between
356.9
1348.4
μm.
Ultrastructure
analysis
revealed
that
internal
cells
lyse
during
maturation
contained
only
a
few
live
embedded
an
extracellular
matrix.
Conidia
produced
by
Solid
State
Fermentation
(SSF)
on
rice
completely
died
1
h
irradiation
with
UV-B
light,
while
84.7%
remained
viable
4
h.
2.5
times
tolerant
heat
than
conidia,
when
exposed
3
at
50
°C.
Storage
trials
showed
viability
rapidly
dropped
months,
months
storage
°C
18
virulent
grains
against
Diatraea
saccharalis
larvae.
Mature
radiation
from
SSF.
fermentation
process
requires
optimization
increase
reducing
time
maintaining
robustness.
In
general,
these
results
led
conclusion
persistent
efficient
harsh
environmental
conditions.
Our
findings
provide
baseline
production
better
understanding
strains.
