Biotechnology for Biofuels,
Год журнала:
2021,
Номер
14(1)
Опубликована: Дек. 17, 2021
As
the
second
most
abundant
polysaccharide
in
nature,
hemicellulose
can
be
degraded
to
xylose
as
feedstock
for
bioconversion
fuels
and
chemicals.
To
enhance
conversion,
engineered
Saccharomyces
cerevisiae
with
metabolic
pathway
is
usually
adapted
carbon
source
laboratory.
However,
mechanism
under
adaptation
phenomena
of
strain
still
unclear.In
this
study,
xylose-utilizing
S.
was
constructed
used
study.
It
found
that
consumption
rate
increased
1.24-fold
incubation
yYST12
synthetic
complete-xylose
medium
compared
first
incubation.
The
study
figured
out
it
observed
at
single-cell
level
stagnation
time
utilization
reduced
after
microfluidic
device.
Such
transient
memory
metabolism
medium,
named
"xylose
memory",
strains
both
isomerase
reductase
xylitol
dehydrogenase
pathways.
In
further,
proteomic
acetylation
before
investigated,
revealed
H4K5
one
differential
sites
related
cerevisiae.
We
tested
8
genes
encoding
acetylase
or
deacetylase,
knockout
GCN5
HPA2
acetylases
enhanced
memory.The
behavior
successfully
induced
adaptation.
H4K5Ac
two
are
during
This
provides
valuable
insights
into
Cells,
Год журнала:
2020,
Номер
9(12), С. 2534 - 2534
Опубликована: Ноя. 24, 2020
A
bottom-up
route
towards
predicting
evolution
relies
on
a
deep
understanding
of
the
complex
network
that
proteins
form
inside
cells.
In
rapidly
expanding
panorama
experimental
possibilities,
most
difficult
question
is
how
to
conceptually
approach
disentangling
such
networks.
These
can
exhibit
varying
degrees
hierarchy
and
modularity,
which
obfuscate
certain
protein
functions
may
prove
pivotal
for
adaptation.
Using
well-established
polarity
in
budding
yeast
as
case
study,
we
first
organize
current
literature
highlight
entrenchments
polarity.
Following
three
examples,
see
alternating
between
novelties
subsequent
emerging
design
strategies
construct
layered
understanding,
potent
enough
reveal
evolutionary
targets.
We
show
if
you
want
understand
cell’s
capacity,
possible
future
paths,
seemingly
unimportant
need
be
mapped
studied.
Finally,
generalize
this
research
structure
applicable
other
systems
interest.
Biotechnology for Biofuels,
Год журнала:
2021,
Номер
14(1)
Опубликована: Дек. 17, 2021
As
the
second
most
abundant
polysaccharide
in
nature,
hemicellulose
can
be
degraded
to
xylose
as
feedstock
for
bioconversion
fuels
and
chemicals.
To
enhance
conversion,
engineered
Saccharomyces
cerevisiae
with
metabolic
pathway
is
usually
adapted
carbon
source
laboratory.
However,
mechanism
under
adaptation
phenomena
of
strain
still
unclear.In
this
study,
xylose-utilizing
S.
was
constructed
used
study.
It
found
that
consumption
rate
increased
1.24-fold
incubation
yYST12
synthetic
complete-xylose
medium
compared
first
incubation.
The
study
figured
out
it
observed
at
single-cell
level
stagnation
time
utilization
reduced
after
microfluidic
device.
Such
transient
memory
metabolism
medium,
named
"xylose
memory",
strains
both
isomerase
reductase
xylitol
dehydrogenase
pathways.
In
further,
proteomic
acetylation
before
investigated,
revealed
H4K5
one
differential
sites
related
cerevisiae.
We
tested
8
genes
encoding
acetylase
or
deacetylase,
knockout
GCN5
HPA2
acetylases
enhanced
memory.The
behavior
successfully
induced
adaptation.
H4K5Ac
two
are
during
This
provides
valuable
insights
into
